pPK705 BioVector®丙酸杆菌穿梭表达质粒载体 BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

pPK705 BioVector®丙酸杆菌穿梭表达质粒载体

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

The pPK705 plasmid is a shuttle vector that can replicate in both Escherichia coli and Propionibacterium species. It is a valuable tool for genetic engineering of Propionibacterium, a genus of bacteria that is important in the food industry and for human health.

The pPK705 plasmid was constructed by cloning the hygromycin B resistance gene (hygB) from the plasmid pUC18 into the plasmid pRGO1, which is a native plasmid from Propionibacterium acidipropionici E214. The pPK705 plasmid also contains the replication origin (ori) and the β-lactamase gene (bla) from pUC18, which allow it to replicate in E. coli.

The pPK705 plasmid has been used to transform a variety of Propionibacterium species, including P. freudenreichii, P. jensenii, and P. thoenii. It has also been used to express heterologous genes in Propionibacterium, such as the gene for the enzyme phosphoenolpyruvate carboxylase (ppc).

The pPK705 plasmid is a valuable tool for genetic engineering of Propionibacterium. It is relatively easy to use and can be used to introduce a variety of genes into Propionibacterium cells. This makes it a useful tool for studying the biology of Propionibacterium and for developing new strains of Propionibacterium for industrial and medical applications.

Map：

生产厂家Supplier:

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

E-mail:BioVector@163.com

http://www.biovector.net