pCC1FOS BioVector®长基因克隆拷贝数可控质粒载体-BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

pCC1FOS BioVector®长基因克隆拷贝数可控质粒载体-BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

BioVector® pCC1FOS is a single-copy vector designed for cloning and maintaining large DNA inserts, such as those derived from genomic DNA or cDNA libraries. It's part of the CopyControl Fosmid system, which offers a unique feature: the ability to induce high-copy number replication when needed.

Key Features of pCC1FOS:

• Single-Copy Origin of Replication: Ensures stability of large inserts, preventing rearrangements and deletions.

• Inducible High-Copy Origin of Replication: Allows for amplification of the plasmid for increased DNA yield when required.

• Chloramphenicol Resistance: Provides selection for cells containing the plasmid.

• Bacteriophage λ Cos Sites: Enables packaging of the plasmid into phage particles for efficient delivery into bacterial cells.

• Multiple Cloning Sites: Offers flexibility for inserting DNA fragments.

Applications of pCC1FOS:

• Cloning Large DNA Inserts: Accommodates large fragments, making it suitable for genomic libraries and BAC clones.

• Stable Maintenance of Clones: The single-copy nature minimizes instability issues.

• High-Yield DNA Preparation: Inducible amplification allows for efficient DNA preparation for sequencing and other downstream applications.

• Functional Genomics: Can be used to study gene function by overexpressing or silencing genes.

Overall, pCC1FOS is a versatile tool for molecular biology research, particularly for handling and manipulating large DNA fragments. Its ability to balance stability and amplification makes it a valuable asset for various applications.

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BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

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