14-124ACL, TLR4/IL-8 NTCC® Leeporter Luciferase Reporter-HeLa Cell Line荧光报告细胞株-BioVector NTCC保藏中心

14-124ACL, TLR4/IL-8 NTCC® Leeporter Luciferase Reporter-HeLa Cell Line荧光报告细胞株-BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

14-124ACL, TLR4/IL-8 Leeporter Luciferase Reporter-HeLa Cell Line is a cell line engineered to study the activation of the Toll-like receptor 4 (TLR4) signaling pathway.

• TLR4: This receptor is a crucial component of the innate immune system, recognizing bacterial lipopolysaccharide (LPS) and triggering an inflammatory response.

  
  

• IL-8: Interleukin-8 is a pro-inflammatory cytokine that plays a key role in recruiting immune cells to sites of infection or injury.

  
  

• Leeporter™: This refers to a specific type of luciferase reporter system. Luciferase is an enzyme that produces light in the presence of its substrate. In this cell line, the luciferase gene is placed under the control of the IL-8 promoter.

  
  

• HeLa Cell Line:This is a widely used immortalized human cell line derived from cervical cancer cells.

  
  

How it works:

1. TLR4 Activation: When the cell line is exposed to a TLR4 agonist, such as LPS, TLR4 is activated.

2. IL-8 Promoter Activation: This activation triggers the transcription of the IL-8 gene.

3. Luciferase Expression: Since the luciferase gene is linked to the IL-8 promoter, its expression is also increased.

4. Luminescence Measurement: The amount of luciferase activity can be measured using a luminometer, providing a quantitative readout of TLR4 signaling.

Applications:

This cell line is a valuable tool for:

• Studying TLR4 signaling pathways: Investigating the mechanisms of TLR4 activation and the downstream signaling events that lead to IL-8 production.

  
  

• Drug discovery:Screening for compounds that can modulate TLR4 signaling, potentially leading to new therapies for inflammatory diseases.

  
  

• Understanding the role of TLR4 in various diseases: Investigating the contribution of TLR4 dysregulation to diseases such as sepsis, arthritis, and inflammatory bowel disease.

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生产厂家Supplier:

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

E-mail:BioVector@163.com

http://www.biovector.net