HT29-MTX-E12 NTCC®经甲氨蝶呤处理的人结肠腺癌细胞株human colon adenocarcinoma cell line BioVector NTCC细胞保藏中心

HT29-MTX-E12 NTCC®经甲氨蝶呤处理的人结肠腺癌细胞株human colon adenocarcinoma cell line

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

The NTCC® HT29-MTX-E12 cell line is a valuable model system in gastrointestinal research, particularly for studying the intestinal mucus layer and its role in various physiological and pathological processes. Here's a breakdown of its key characteristics and significance:

Key Characteristics:

• Origin: Derived from the human colon adenocarcinoma cell line HT29 through treatment with methotrexate (MTX).

• Mucus Production: HT29-MTX-E12 cells are known for their ability to produce a thick mucus layer, which closely resembles the composition and structure of the human intestinal mucus.

• Mucin Expression: These cells express various mucins, including MUC2, MUC5AC, and MUC6, which are key components of the intestinal mucus.

• Tight Junction Formation: HT29-MTX-E12 cells form tight junctions, creating a barrier similar to the intestinal epithelium.

Significance:

• Mucus Research: The cell line provides a valuable in vitro model to study the mechanisms of mucus production, composition, and regulation.

• Host-Pathogen Interactions: HT29-MTX-E12 cells are used to investigate the interactions between the intestinal epithelium and pathogens, such as bacteria and viruses.

• Drug Delivery: The mucus layer plays a crucial role in drug absorption and delivery. This cell line can be used to study how drugs interact with the mucus and to develop more effective drug delivery systems.

• Inflammatory Bowel Disease (IBD): The cell line is employed to model IBD, a chronic inflammatory condition affecting the gastrointestinal tract. By studying the behavior of HT29-MTX-E12 cells under inflammatory conditions, researchers can gain insights into the pathogenesis of IBD.

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Visual Representation:

HT29MTXE12 cells forming a mucus layer