N/TERT-1永生化人角质形成细胞株immortalized human keratinocyte cell line BioVector NTCC®典型培养物保藏中心

主要特点

• 来源： 源自正常人新生儿包皮角质形成细胞，通过表达人端粒酶逆转录酶（hTERT）基因实现永生化。

• 分化能力： 该细胞系保留了分化能力，可以在3D类器官细胞培养模型中形成完全分层的、功能性的表皮（称为人表皮等效物，HEEs），形态上非常接近正常的成人皮肤。

• 核型： N/TERT-1细胞系为二倍体，并多一条20号染色体（47, XY, +20）。

• 应用： 它是研究表皮生物学、炎症性皮肤病（如银屑病和特应性皮炎）的发病机制、毒理学以及药物筛选的重要工具。

• 减少变异性： 与原代角质形成细胞不同，原代细胞存在显著的供体间差异，而N/TERT-1细胞提供了稳定且无限的细胞来源，这对于大规模和可重复的研究至关重要。

• 易于基因操作： 该细胞系易于进行基因编辑技术（如CRISPR-Cas9）操作，使研究人员能够研究特定基因在皮肤生物学和疾病中的功能。

• 优于HaCaT细胞系： 研究表明，在形成生物学相关模型方面，N/TERT-1细胞优于广泛使用的HaCaT细胞系，尤其是在分化和对炎症刺激的反应方面。

NTCC® N/TERT-1 cell line is an immortalized human keratinocyte cell line widely used in dermatology and skin biology research as a stable and reproducible alternative to primary human keratinocytes.

• Origin: Derived from normal human neonatal foreskin keratinocytes that were immortalized by the expression of the human telomerase reverse-transcriptase (hTERT) gene.

• Differentiation: The cells retain the ability to differentiate and form a fully stratified, functional epidermis (known as human epidermal equivalents, or HEEs) in 3D organotypic cell culture models, closely mimicking normal human skin morphology and barrier properties.

• Karyotype: The N/TERT-1 cell line is diploid with an additional chromosome 20 (47, XY, +20).

• Usage: It is a valuable tool for studying epidermal biology, the pathogenesis of inflammatory skin diseases (such as psoriasis and atopic dermatitis), toxicology, and for drug screening purposes.

• Reduced Variability: Unlike primary keratinocytes, which show significant donor-to-donor variability, N/TERT-1 cells provide a consistent and unlimited cell source, which is crucial for large-scale and reproducible research.

• Genetic Amenability: The cell line is amenable to genetic manipulation techniques like CRISPR-Cas9, allowing researchers to study the function of specific genes in skin biology and disease.

• Superiority to HaCaT: Research suggests that N/TERT-1 cells form more biologically relevant models than the widely used HaCaT cell line, particularly in terms of differentiation and response to inflammatory stimuli.

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

BioVector NTCC Inc.

TEL: 400-800-2947, 189-0126-8599

E-mail: biovector@163.com

http://www.biovector.net