H9-Nurr1::H2B-GFP-iCas9-LRRK2G2019S/G2019S细胞株NTCC® Cell Line BioVector细胞保藏中心

[Designation名称] NTCC® H9-Nurr1::H2B-GFP-iCas9-LRRK2G2019S/G2019S Cell Line细胞株
BioVector NTCC Inc.

Synonyms细胞别名: ID H9 NR4A2-eGFP iCas9 LRRK2(G2019S/G2019S)
Comments: Characteristics: Cell line with robust Dox-inducible expression of Cas9 and an edited homozygous LRRK2 (G2019S) mutation (PubMed=38917806).
Accession Number: NTCC® CVCL_E7GK
References identifiers: PubMed=38917806;
Comments: Characteristics: Using CRISPR/Cas9 a P2A-H2B-eGFP-PGKp-PuroR construct was introduced at the C-terminus of NR4A2/NURR1 (from parent cell line).
Comments: Characteristics: Using TALEN two constructs SA-2A-HygR-Cas9-TRE and SA-2A-Neo-M2rtTA were introduced in the AAVS1 safe harbor locus, one in each allele (from parent cell line).
Comments: Sequence variation: Mutation; HGNC; HGNC:18618; LRRK2; Simple_edited; p.Gly2019Ser (c.6055G>A); ClinVar=VCV000001940; Zygosity=Homozygous; Note=By CRISPR/Cas9 (PubMed=38917806).
Comments: Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Comments: Genetic integration: Method=CRISPR/Cas9; Gene=HGNC; HGNC:4761; H2BC11.
Comments: Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Comments: Genetic integration: Method=TALEN; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Comments: Genetic integration: Method=TALEN; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Comments: Genetic integration: Method=TALEN; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
Comments: Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Comments: Cell type: Embryonic stem cell; CL=CL_0002322.
[Organism物种来源]
[Disease疾病种类]
[Accession编号] NTCC®-Accession Number: NTCC® CVCL_E7GK
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[Reference参考文献]
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[Date保藏时间]
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培养方法：
Medium培养基：NTCC® H9-Nurr1::H2B-GFP-iCas9-LRRK2G2019S/G2019S Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址：www.biovector.net