pCCL.sin.cPPT.EF1α.WPRE BioVector® Plasmid第三代自失活（SIN）慢病毒转移包装质粒

BioVector® pCCL.sin.cPPT.EF1α.WPRE Plasmid第三代自失活（SIN）慢病毒转移包装质粒

1. Vector Overview

• Full Name: BioVector® pCCL.sin.cPPT.EF1α.WPRE

• Type: Third-generation, self-inactivating (SIN) lentiviral transfer plasmid.

• Parental Backbone: Derived from the pCCL family of vectors, developed for gene therapy and stable gene transfer.

• Key Application: Used to package lentiviral particles for efficient delivery and stable expression of transgenes in mammalian cells (including dividing and non-dividing cells).

2. Genetic Elements and Features
The plasmid contains several critical functional elements essential for lentiviral production and gene expression:

• 5' LTR (Long Terminal Repeat): Contains the HIV-1 LTR sequence. During packaging, it is replaced by a chimeric promoter (RSV/U3) in the packaging plasmid to initiate transcription of the viral RNA genome.

• Ψ (Psi) Packaging Signal: The primary sequence required for the viral genomic RNA to be packaged into the lentiviral capsid.

• cPPT (central Polypurine Tract): Enhances nuclear import of the viral pre-integration complex, significantly improving transduction efficiency in non-dividing cells.

• EF1α Promoter: Drives the expression of the gene of interest. The human Elongation Factor 1 alpha (EF1α) promoter provides strong and stable expression in a wide range of cell types.

• WPRE (Woodchuck Hepatitis Virus Post-transcriptional Regulatory Element): Enhances transgene expression by increasing RNA stability and facilitating nuclear export of transcripts.

• 3' SIN LTR (Self-Inactivating LTR): Contains a deletion in the U3 region. After reverse transcription and integration into the host genome, this deletion is copied to the 5' LTR, inactivating promoter activity in the provirus to enhance biosafety and prevent mobilization.

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3. Mechanism and Applications

• Lentiviral Production: When co-transfected into packaging cells (e.g., HEK293T) with helper plasmids (packaging and envelope), this vector generates replication-incompetent lentiviral particles.

• Transduction: These particles can transduce target cells. The cPPT facilitates nuclear import, and the EF1α promoter ensures sustained transgene expression.

• Safety: The SIN design reduces the risk of insertional activation of oncogenes.

质粒：BioVector® pCCL.sin.cPPT.EF1α.WPRE

1. 载体概述

• 全称： BioVector® pCCL.sin.cPPT.EF1α.WPRE

• 类型： 第三代自失活（SIN）慢病毒转移质粒。

• 亲本骨架： 源于pCCL载体家族，专为基因治疗和稳定基因转移而开发。

• 主要应用： 用于包装慢病毒颗粒，以在哺乳动物细胞（包括分裂细胞和非分裂细胞）中高效递送和稳定表达目标基因。

2. 基因元件与特征
该质粒包含多个对慢病毒生产和基因表达至关重要的功能元件：

• 5' LTR (长末端重复序列)： 包含HIV-1 LTR序列。在包装过程中，该序列会被包装质粒中的嵌合启动子（RSV/U3）替代，以启动病毒RNA基因组的转录。

• Ψ (Psi) 包装信号： 病毒基因组RNA被包装入慢病毒衣壳所需的主要序列。

• cPPT (中心多嘌呤束)： 增强病毒预整合复合物的核输入，显著提高对非分裂细胞的转导效率。

• EF1α 启动子： 驱动目标基因的表达。人源延伸因子1 alpha (EF1α) 启动子在多种细胞类型中提供强健且稳定的表达。

• WPRE (土拨鼠肝炎病毒转录后调控元件)： 通过增加RNA稳定性和促进转录本的核输出来增强转基因表达。

• 3' SIN LTR (自失活LTR)： U3区域存在缺失。在逆转录并整合到宿主基因组后，该缺失会被复制到5' LTR，从而灭活前病毒的启动子活性，增强生物安全性并防止载体 mobilization。

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3. 机制与应用

• 慢病毒生产： 当与辅助质粒（包装质粒和包膜质粒）共转染到包装细胞（如HEK293T）中时，该载体可生成具有复制缺陷能力的慢病毒颗粒。

• 转导： 这些颗粒可以转导靶细胞。cPPT元件促进核输入，EF1α启动子则确保转基因的持续表达。

• 安全性： SIN设计降低了插入激活致癌基因的风险。

Supplier生产商：

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

电话:400-800-2947

工作QQ/微信同号:1843439339

网址 www.biovector.net