Vero-P15 BioVector® Cell Line非洲绿猴肾脏上皮细胞株

BioVector® Vero-P15 Cell Line

English Version

1. Cell Line Overview

• Cell Line Name: BioVector® Vero-P15

• Species of Origin:Chlorocebus sabaeus (African green monkey)

• Tissue Origin: Kidney

• Cell Type: Epithelial

• Morphology: Adherent (Parental); P15 specifically refers to a characterized adherent passage level

• Parental Line: Derived from the original Vero (Verda Reno) cell line, established from the kidney of a normal adult African green monkey

• ATCC Number: CCL-81 (Parental Vero lineage)

2. Description and Applications
BioVector® Vero-P15 represents a specific, well-characterized passage (Passage 15) of the Vero cell line. Vero cells are one of the most widely used continuous cell lines in virology and vaccine production. Approved by the World Health Organization (WHO) for vaccine manufacturing, Vero cells are highly susceptible to a broad range of viruses. The P15 designation indicates cells that have been carefully maintained to ensure genetic stability and consistent performance in viral susceptibility assays and production workflows.

3. Key Features

• WHO-Approved Substrate: Accepted for use in human and veterinary vaccine production.

• Broad Virus Susceptibility: Supports replication of numerous viruses including poliovirus, respiratory syncytial virus (RSV), yellow fever virus, influenza viruses, and many others.

• Interferon Deficiency: Vero cells are naturally deficient in interferon production, making them exceptionally sensitive to viral infection.

• Genetic Stability: P15 represents a passage level with well-documented stability and characterization.

4. Growth and Culture Conditions

• Recommended Medium: BioVector® High-Glucose DMEM or BioVector® Medium 199, supplemented with 5-10% fetal bovine serum (FBS).

• Growth Characteristics: Adherent cells with a doubling time of approximately 24-28 hours. Requires standard incubation conditions (37°C, 5% CO₂).

• Subculture Ratio: Typically split at a ratio of 1:4 to 1:6 when confluent.

• Adaptation Potential: Parental adherent Vero cells (including P15) can be adapted to suspension culture using specific media formulations for large-scale bioreactor applications.

5. Quality Control and Safety

• Sterility Testing: Negative for bacteria, fungi, and mycoplasma.

• Viral Testing: Tested and confirmed negative for adventitious viral agents.

• Tumorigenicity: Vero cells are non-tumorigenic at early passages (including P15) but may become tumorigenic at very high passages; therefore, passage control is critical.

6. Applications

• Isolation and propagation of human and animal viruses

• Production of viral vaccines (poliovirus, rabies, rotavirus, etc.)

• Plaque assays and virus titration

• Virus-host interaction studies

• Cytopathic effect (CPE) assays

• Antiviral drug screening and testing

BioVector® Vero-P15 细胞系

中文版本

1. 细胞系概述

• 细胞系名称： BioVector® Vero-P15

• 物种来源：Chlorocebus sabaeus (非洲绿猴)

• 组织来源： 肾脏

• 细胞类型： 上皮细胞

• 形态特征： 贴壁生长（亲本型）；P15特指一个有明确特征描述的贴壁培养代次

• 亲本系： 源自原始的Vero（Verda Reno）细胞系，该细胞系建立自一只正常成年非洲绿猴的肾脏

• ATCC编号： CCL-81（亲本Vero谱系）

2. 描述与应用
BioVector® Vero-P15 代表了一个特定、充分鉴定的Vero细胞传代次（第15代）。Vero细胞是病毒学和疫苗生产中使用最广泛的连续细胞系之一。经世界卫生组织（WHO）批准用于疫苗生产，Vero细胞对多种病毒高度敏感。P15这一名称表示细胞经过了精心维护，以确保在病毒敏感性测定和生产流程中的遗传稳定性和性能一致性。

3. 主要特征

• WHO批准底物： 被接受用于人用和兽用疫苗生产。

• 广谱病毒敏感性： 支持多种病毒的复制，包括脊髓灰质炎病毒、呼吸道合胞病毒（RSV）、黄热病病毒、流感病毒等。

• 干扰素缺陷： Vero细胞天然存在干扰素产生缺陷，使其对病毒感染格外敏感。

• 遗传稳定性： P15代表了具有充分文献记载的稳定性和特征描述的代次水平。

4. 生长与培养条件

• 推荐培养基： BioVector® 高糖DMEM 或 BioVector® Medium 199，添加5-10%胎牛血清（FBS）。

• 生长特性： 贴壁细胞，倍增时间约为24-28小时。需要标准培养条件（37°C，5% CO₂）。

• 传代比例： 通常在细胞长满后按1:4至1:6的比例进行传代。

• 驯化潜力： 亲本贴壁Vero细胞（包括P15）可以使用特定的培养基配方，驯化为悬浮培养，用于大规模生物反应器应用。
  

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5. 质量控制与安全性

• 无菌检测： 细菌、真菌和支原体检测均为阴性。

• 病毒检测： 经过检测，确证无异源病毒因子。

• 致瘤性： Vero细胞在早期传代（包括P15）是非致瘤性的，但在非常高代次时可能具有致瘤性；因此，严格控制传代次数至关重要。

6. 应用

• 人源和动物病毒的分离与扩增

• 病毒疫苗生产（脊髓灰质炎病毒、狂犬病毒、轮状病毒等）

• 噬斑测定和病毒滴定

• 病毒与宿主相互作用研究

• 细胞病变效应（CPE）测定

• 抗病毒药物筛选和测试

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