Vero F68 BioVector® Cell Line非洲绿猴肾脏上皮悬浮驯化细胞珠-用于病毒疫苗和生物制品的大规模工业生产-Biovector NTCC保藏中心

BioVector® Vero F68 Cell Line

English Version

1. Cell Line Overview

• Cell Line Name: BioVector® Vero F68

• Species of Origin:Chlorocebus sabaeus (African green monkey)

• Tissue Origin: Kidney

• Cell Type: Epithelial

• Morphology: Adapted for suspension growth in serum-free media

• Parental Line: Derived from the adherent Vero (Verda Reno) cell line (ATCC CCL-81) through specific adaptation and selection

• Deposit Institution: ECACC 88052002 (Parental information)

2. Description and Applications
BioVector® Vero F68 is a suspension-adapted variant of the classic Vero cell line, specifically selected for its ability to grow in serum-free medium. It was originally developed and is widely used for large-scale industrial production of viral vaccines and biologics. The F68 designation indicates a cell line that has been successfully adapted to suspension culture, eliminating the need for microcarriers and facilitating high-density growth in bioreactors. This cell line retains the broad viral susceptibility of its parental adherent line while enabling scalable, cost-effective manufacturing processes.

3. Key Features

• Suspension-Adapted: Grows as single cells or small aggregates in suspension without microcarriers.

• Serum-Free Capable: Adapted for growth in chemically defined, serum-free media, reducing animal-derived component risks.

• WHO-Approved Substrate: Accepted for use in human vaccine production.

• Scalability: Ideal for large-scale bioreactor cultures up to thousands of liters.

• Broad Virus Susceptibility: Retains the ability to support replication of numerous viruses including poliovirus, rabies virus, influenza viruses, and SARS-CoV-2.

4. Growth and Culture Conditions

• Recommended Medium: BioVector® Serum-Free Vero Suspension Medium or BioVector® VP-SFM (Virus Production Serum-Free Medium).

• Growth Characteristics: Suspension cells with a doubling time of approximately 24-30 hours in optimized media. Requires standard incubation conditions (37°C, 5% CO₂) with agitation (100-150 rpm in shake flasks).

• Culture Maintenance: Maintain cell density between 2 × 10⁵ and 3 × 10⁶ cells/mL. Subculture when density reaches 2-3 × 10⁶ cells/mL.

• Adaptation Note: Cells are already suspension-adapted; however, gradual adaptation to new media formulations may be necessary for optimal performance.

5. Quality Control and Safety

• Sterility Testing: Negative for bacteria, fungi, and mycoplasma.

• Viral Testing: Tested and confirmed negative for adventitious viral agents.

• Tumorigenicity: As a suspension-adapted continuous cell line, rigorous purification of final products is required to remove residual cells and DNA.

6. Applications

• Large-scale industrial production of viral vaccines (polio, rabies, influenza, rotavirus)

• Production of viral vectors for gene therapy

• Virus antigen production for diagnostic assays

• High-yield virus replication studies

• Bioprocess development and optimization

• Recombinant protein production

BioVector® Vero F68 细胞系说明书

中文版本

1. 细胞系概述

• 细胞系名称： BioVector® Vero F68

• 物种来源：Chlorocebus sabaeus (非洲绿猴)

• 组织来源： 肾脏

• 细胞类型： 上皮细胞

• 形态特征： 适应于在无血清培养基中悬浮生长

• 亲本系： 源自贴壁生长的经典Vero（Verda Reno）细胞系（ATCC CCL-81），通过特定的适应和筛选获得

• 保藏机构： ECACC 88052002（亲本信息）

2. 描述与应用
BioVector® Vero F68 是经典Vero细胞系的一个悬浮驯化变体，因其在无血清培养基中生长的能力而被特别筛选出来。它最初被开发并广泛用于病毒疫苗和生物制品的大规模工业生产。F68这一名称表示该细胞系已成功适应悬浮培养，消除了对微载体的需求，并促进了生物反应器中的高密度生长。该细胞系保留了其亲本贴壁细胞的广谱病毒敏感性，同时实现了可规模化、成本效益高的生产工艺。

3. 主要特征

• 悬浮驯化： 无需微载体，以单细胞或小聚集体形式在悬浮状态下生长。

• 无血清能力： 已适应在化学成分明确、无血清的培养基中生长，降低了动物源成分风险。

• WHO批准底物： 被接受用于人用疫苗生产。

• 可规模化： 适用于高达数千升的大规模生物反应器培养。

• 广谱病毒敏感性： 保留支持多种病毒复制的能力，包括脊髓灰质炎病毒、狂犬病毒、流感病毒和SARS-CoV-2。

4. 生长与培养条件

• 推荐培养基： BioVector® 无血清Vero悬浮培养基 或 BioVector® VP-SFM（病毒生产无血清培养基）。

• 生长特性： 在优化培养基中，悬浮细胞的倍增时间约为24-30小时。需要标准培养条件（37°C，5% CO₂），并伴随搅动（摇瓶中100-150 rpm）。

• 培养维持： 维持细胞密度在2 × 10⁵ 至 3 × 10⁶ 细胞/毫升之间。当密度达到2-3 × 10⁶ 细胞/毫升时进行传代。

• 适应说明： 细胞已经过悬浮驯化；然而，为了获得最佳性能，可能需要逐步适应新的培养基配方。

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5. 质量控制与安全性

• 无菌检测： 细菌、真菌和支原体检测均为阴性。

• 病毒检测： 经过检测，确证无异源病毒因子。

• 致瘤性： 作为一种悬浮驯化的连续细胞系，需要对最终产品进行严格的纯化，以去除残留细胞和DNA。

6. 应用

• 病毒疫苗的大规模工业化生产（脊髓灰质炎、狂犬病、流感、轮状病毒）

• 用于基因治疗的病毒载体生产

• 用于诊断检测的病毒抗原生产

• 高产量病毒复制研究

• 生物工艺开发与优化

• 重组蛋白生产

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

电话:400-800-2947

工作QQ/微信同号:1843439339

网址 www.biovector.net