MDCK 585 BioVector® Cell Line犬肾脏上皮细胞株-用于流感病毒疫苗生产-BioVector NTCC保藏中心

BioVector® MDCK 585 Cell Line

English Version

1. Cell Line Overview

• Cell Line Name: BioVector® MDCK 585

• Species of Origin:Canis familiaris (Canine)

• Tissue Origin: Kidney

• Cell Type: Epithelial

• Morphology: Adherent

• Parental Line: Derived from the original MDCK (Madin-Darby Canine Kidney) cell line

2. Description and Applications
BioVector® MDCK 585 is a specific isolate or subclone of the MDCK cell line, referenced in research contexts involving viral replication studies. The designation "585" may refer to a laboratory-specific clone or a passage-level variant selected for particular research applications. MDCK cells are widely used as a gold standard substrate for influenza virus isolation, propagation, and vaccine production due to their high susceptibility to human and avian influenza viruses.

3. Key Features

• Virus Susceptibility: Supports efficient replication of influenza A and B viruses

• Research Application: Used in virology research, particularly for influenza virus studies

• Cytopathic Effect (CPE): Exhibits clear and reproducible CPE upon viral infection, facilitating titration and plaque assays

4. Growth and Culture Conditions

• Recommended Medium: BioVector® MEM (Eagle's Minimum Essential Medium) or BioVector® High-Glucose DMEM, supplemented with 5-10% fetal bovine serum (FBS)

• Growth Characteristics: Adherent epithelial cells forming monolayers; doubling time approximately 24 hours under optimal conditions

• Culture Conditions: 37°C, 5% CO₂

• Subculture Ratio: Typically split at 1:3 to 1:6 when confluent (every 3-4 days)

• Subculture Method: Trypsin-EDTA solution

5. Quality Control

• Sterility Testing: Negative for bacteria, fungi, and mycoplasma

• Species Verification: Canine origin confirmed

6. Applications

• Influenza virus isolation and propagation

• Viral titration and plaque assays

• Antiviral drug screening

• Virus-host interaction studies

• Vaccine development research

BioVector® MDCK 585 细胞系说明书

中文版本

1. 细胞系概述

• 细胞系名称： BioVector® MDCK 585

• 物种来源： 犬

• 组织来源： 肾脏

• 细胞类型： 上皮细胞

• 形态特征： 贴壁生长

• 亲本系： 源自原始的MDCK（马-达二氏犬肾）细胞系

2. 描述与应用
BioVector® MDCK 585是MDCK细胞系的一个特定分离株或亚克隆，在涉及病毒复制研究的研究文献中被引用。"585"这一名称可能指的是为特定研究应用而选择的实验室特异性克隆或传代变异体。MDCK细胞因其对人源和禽流感病毒的高度敏感性，被广泛用作流感病毒分离、扩增和疫苗生产的金标准底物。

3. 主要特征

• 病毒敏感性： 支持甲型和乙型流感病毒的高效复制

• 研究应用： 用于病毒学研究，特别是流感病毒研究

• 细胞病变效应： 病毒感染后表现出清晰且可重复的细胞病变效应，便于滴定和噬斑测定

4. 生长与培养条件

• 推荐培养基： BioVector® MEM（Eagle's基础培养基）或BioVector® 高糖DMEM，添加5-10%胎牛血清（FBS）

• 生长特性： 贴壁上皮细胞，形成单层；优化条件下倍增时间约为24小时

• 培养条件： 37°C，5% CO₂

• 传代比例： 通常在细胞长满后按1:3至1:6的比例传代（每3-4天）

• 传代方法： 胰蛋白酶-EDTA溶液

5. 质量控制

• 无菌检测： 细菌、真菌和支原体检测均为阴性

• 物种鉴定： 确认为犬源

6. 应用

• 流感病毒的分离与扩增

• 病毒滴定和噬斑测定

• 抗病毒药物筛选

• 病毒与宿主相互作用研究

• 疫苗开发研究

Note: The MDCK 585 designation appears in research literature in the context of influenza virus studies, specifically referencing viral strains such as A/Chicken/Malaysia/5858/2004 (H5N1). This suggests the cell line may be associated with studies using that particular viral isolate.

说明： MDCK 585这一名称出现在流感病毒研究的文献中，具体涉及诸如A/Chicken/Malaysia/5858/2004（H5N1）等病毒株。这表明该细胞系可能与使用该特定病毒分离株的研究相关。

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