pAmpho Plasmid BioVector®逆转录病毒包膜表达载体-专用于生产嗜异性逆转录病毒BioVector NTCC典型培养物保藏中心

Product Name / 产品名称:
BioVector® pAmpho Plasmid逆转录病毒包膜表达载体，专用于生产嗜异性逆转录病毒
BioVector® pAmpho质粒

Description / 描述:
The BioVector® pAmpho plasmid is a retroviral envelope expression vector designed for the production of amphotropic retroviruses. It expresses the 4070A amphotropic envelope protein under the control of the CMV immediate-early promoter . The amphotropic envelope protein enables viral particles to infect a wide range of mammalian cells by interacting with the RAM-1 receptor on the host cell surface . This plasmid is commonly used in conjunction with retroviral packaging cell lines and helper constructs to generate replication-incompetent retroviruses for gene delivery .
BioVector® pAmpho 质粒是一种逆转录病毒包膜表达载体，专用于生产嗜异性逆转录病毒。它在CMV立即早期启动子的控制下表达4070A嗜异性包膜蛋白 。该嗜异性包膜蛋白通过与宿主细胞表面的RAM-1受体相互作用，使病毒颗粒能够感染广泛的哺乳动物细胞 。该质粒通常与逆转录病毒包装细胞系和辅助构建体联合使用，以产生用于基因递送的无复制能力的逆转录病毒 。

Original Source / 原始来源:
Clontech (now Takara Bio) / Clontech公司（现Takara Bio）

Plasmid Type / 质粒类型:
Retroviral Envelope Expression Vector / 逆转录病毒包膜表达载体

Species / 物种:
Mammalian expression / 哺乳动物表达

Vector Backbone / 载体骨架:
pUC-based / pUC系列骨架

Total Vector Size / 总载体大小:
6732 bp

Key Components / 关键组件:

Gene/Insert Details / 基因/插入片段详情:

Growth Conditions in Bacteria / 细菌生长条件:

Applications / 应用:

• Retroviral Packaging / 逆转录病毒包装: Used with packaging cell lines (e.g., GP2-293) and transfer vectors to produce amphotropic retroviruses

• Gene Delivery / 基因递送: Enables infection of a broad range of mammalian cells including human, mouse, rat, and hamster cells

• Viral Pseudotyping / 病毒假型化: Provides amphotropic envelope for pseudotyping retroviral and lentiviral vectors

• Stable Cell Line Generation / 稳定细胞系构建: Facilitates generation of stable cell lines via retroviral transduction

• Gene Function Studies / 基因功能研究: Delivers genes of interest to difficult-to-transfect cell types

Related Products / 相关产品:

Quality Control / 质量控制:
Plasmid is verified by restriction digestion and sequencing. Full sequence information is available from the manufacturer.
质粒经过限制性酶切和测序验证。完整序列信息可向制造商获取。

Storage / 存储:

• Plasmid is sent as a bacterial stab in agar or as purified DNA. Upon receipt, streak onto a plate with BioVector® Ampicillin (100 μg/mL), grow a single colony, and prepare a glycerol stock for long-term storage at -80°C.

• 质粒以细菌穿刺菌或纯化DNA形式提供。收到后，应在含 BioVector® 氨苄青霉素 (100 μg/mL) 的平板上划线，挑取单克隆，并制备甘油菌于-80°C长期保存。

Plasmid Reconstitution Protocol / 质粒溶解复配方案:

1. Centrifuge at 5,000×g for 5 min. / 5,000×g离心5分钟。

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA. / 小心打开管盖，加入20 μl无菌水溶解DNA。

3. Close the tube and incubate for 10 minutes at room temperature. / 盖紧管盖，室温孵育10分钟。

4. Briefly vortex and spin down. / 短暂涡旋并离心收集。

5. Store the plasmid at -20°C. / 于-20°C保存质粒。

6. It is recommended to transform and extract fresh plasmid before use. / 建议使用前进行转化并提取新鲜质粒。

General Plasmid Transformation Protocol / 通用质粒转化方案:

1. Thaw 100 μl competent cells on ice for 10 min, add 2 μl plasmid, incubate on ice for 30 min, heat-shock at 42°C for 60 sec, then incubate on ice for 2 min. / 取100 μl感受态细胞冰浴10分钟，加入2 μl质粒，冰浴30分钟，42°C热激60秒，再冰浴2分钟。

2. Add 900 μl antibiotic-free LB medium, incubate at 37°C for 45 min with shaking at 180 rpm. / 加入900 μl无抗生素LB培养基，37°C 180 rpm振荡培养45分钟。

3. Centrifuge at 6,000 rpm for 5 min, resuspend pellet in 100 μl supernatant, spread onto LB plate with appropriate antibiotic. / 6,000 rpm离心5分钟，用100 μl上清重悬沉淀，涂布于含相应抗生素的LB平板。

4. Incubate plate inverted at 37°C for 14 hours. / 倒置平板，37°C培养14小时。

5. Pick single colony into LB medium with antibiotic, incubate at 220 rpm for 14 hours, extract plasmid. / 挑取单克隆至含抗生素的LB培养基，220 rpm培养14小时，提取质粒。

Citation / 引用:
When using this plasmid, please cite the following publications as appropriate:
使用此质粒时，请根据情况引用以下文献：

Miller AD, Rosman GJ. Improved retroviral vectors for gene transfer and expression. Biotechniques. 1989 Oct;7(9):980-2, 984-6, 989-90.

Important Note / 重要提示:

• The pAmpho plasmid is designed for research use only and should be handled under appropriate biosafety conditions (BSL-1 or BSL-2 depending on the viral constructs used) .

• pAmpho质粒仅限研究使用，应在适当的生物安全条件下操作（根据所用病毒构建体的不同，为BSL-1或BSL-2级）。

• For retrovirus production, pAmpho must be co-transfected with a packaging construct (expressing gag-pol) and a transfer vector containing the gene of interest .

• 用于逆转录病毒生产时，pAmpho必须与包装构建体（表达gag-pol）和含有目的基因的转移载体共转染 。

• The amphotropic envelope enables infection of many mammalian cell types, but VSV-G pseudotyping offers an even broader host range .

• 嗜异性包膜能够感染多种哺乳动物细胞类型，但VSV-G假型化提供更广泛的宿主范围 。

BioVector® Ampicillin
BioVector® 氨苄青霉素

For bacterial selection of the pAmpho plasmid and its derivatives.
用于pAmpho质粒及其衍生质粒的细菌筛选。

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

电话:400-800-2947

工作QQ/微信同号:1843439339

网址http://www.biovector.net