BioVector® SM10 λpir大肠杆菌接合/供体菌株/感受态细胞SM10 λpir Strain

BioVector® SM10 λpir 大肠杆菌接合/供体菌株 / SM10 λpir Strain 数据说明书

产品名称: BioVector® SM10 λpir 感受态细胞
中文名称: BioVector® SM10 λpir 大肠杆菌接合/供体菌株
英文名称: BioVector® SM10 λpir E. coli Conjugative/Donor Strain
产品类别: 感受态细胞/供体菌株
菌株类型: 大肠杆菌 (E. coli)
菌株用途: 适用于作为携带R6K复制起始位点（ori）的穿梭质粒或自杀性质粒的接合转移供体菌株，常用于基因敲除、细菌遗传学操作和转座子诱变
菌株基因型: thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu (Kmᵣ) λpir
特性特征:

1. λpir 溶原菌: 表达π蛋白（pir基因产物），为携带R6K复制起始位点的质粒提供反式复制必需蛋白，使此类质粒能够在该菌株中稳定维持

2. 接合转移功能: 含有整合在染色体上的RP4接合转移系统基因（RP4-2-Tc::Mu），能够高效地将携带oriT（转移起始位点）的质粒通过接合作用转移到受体菌株（如假单胞菌、沙门氏菌等其他革兰氏阴性菌）

3. recA1: 缺失同源重组能力，维持质粒在供体菌中的稳定性

4. 链霉素敏感: 便于接合后利用链霉素进行受体菌筛选（SM10本身对链霉素敏感，而许多受体菌具有链霉素抗性）

5. 抗性标记: 染色体上含有卡那霉素抗性基因（Kmᵣ）
   质控载体: BioVector® pR6K-oriT 质粒（含R6K复制起始位点和接合转移起始位点oriT）
   接合转移效率: 经测试，可高效转移至受体菌株
   质量控制: 接合转移功能验证：阳性；无菌检测：阴性；抗性标记验证：卡那霉素抗性
   储存与运输: -80°C或干冰运输与保存
   仅供科研使用

English:
BioVector® SM10 λpir Strain / Competent Cells Datasheet

Product Name: BioVector® SM10 λpir Competent Cells
Chinese Name: BioVector® SM10 λpir 大肠杆菌接合/供体菌株
English Name: BioVector® SM10 λpir E. coli Conjugative/Donor Strain
Product Category: Competent Cells / Donor Strain
Strain Type: E. coli (Escherichia coli)
Strain Application: Serves as a donor strain for conjugative transfer of shuttle or suicide plasmids containing the R6K replication origin (ori). Commonly used in gene knockout, bacterial genetics, and transposon mutagenesis
Strain Genotype: thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu (Kmᵣ) λpir
Characteristics:

1. λpir Lysogen: Expresses the π protein (product of the pir gene), which provides the essential replication protein in trans for plasmids carrying the R6K origin of replication, enabling stable maintenance of such plasmids in this strain

2. Conjugative Transfer Function: Contains the RP4 conjugation system genes (RP4-2-Tc::Mu) integrated into the chromosome, enabling efficient transfer of plasmids carrying oriT (origin of transfer) to recipient strains (e.g., Pseudomonas, Salmonella, and other Gram-negative bacteria) via conjugation

3. recA1: Lacks homologous recombination activity, maintaining plasmid stability in the donor strain

4. Streptomycin Sensitive: Facilitates counterselection of recipient strains after conjugation using streptomycin (SM10 is streptomycin sensitive, while many recipient strains are streptomycin resistant)

5. Selection Marker: Contains a kanamycin resistance gene (Kmᵣ) on the chromosome
   Control Vector: BioVector® pR6K-oriT Plasmid (containing R6K replication origin and oriT transfer origin)
   Conjugation Efficiency: Tested and validated for efficient transfer to recipient strains
   Quality Control: Conjugation Function Validation: Positive; Sterility: Negative; Selection Marker Validation: Kanamycin Resistance
   Storage and Shipment: Stored and shipped at -80°C or on dry ice
   For Research Use Only

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