BioVector® DH5α λpir大肠杆菌菌株-用于R6K复制起始位点的质粒（基因敲除载体、自杀性质粒）克隆和扩增

BioVector® DH5α λpir大肠杆菌菌株-适用于携带R6K复制起始位点（ori）的质粒（如基因敲除载体、自杀性质粒）的克隆和扩增 / DH5α λpir Strain 数据说明书

产品名称: BioVector® DH5α λpir 感受态细胞
中文名称: BioVector® DH5α λpir 大肠杆菌克隆菌株
英文名称: BioVector® DH5α λpir E. coli Cloning Strain
产品类别: 感受态细胞/克隆菌株
菌株类型: 大肠杆菌 (E. coli)
菌株用途: 适用于携带R6K复制起始位点（ori）的质粒（如基因敲除载体、自杀性质粒）的克隆和扩增
菌株基因型: F- endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG purB20 φ80dlacZΔM15 Δ(lacZYA-argF)U169 λ-
特性特征:

1. λpir 溶原菌: 表达π蛋白（pir基因产物），为携带R6K复制起始位点的质粒提供反式复制必需蛋白，使此类质粒能够在该菌株中稳定复制和维持

2. recA1 和 endA1: 缺失同源重组能力，提高质粒插入片段的稳定性；缺失非特异性核酸内切酶活性，提高质粒提取产量和质量

3. lacZΔM15: 可与pUC系列载体实现蓝白斑筛选
   质控载体: BioVector® pR6K 质粒（含R6K复制起始位点）
   转化效率: ≥ 1 x 10⁷ cfu/µg pR6K质粒DNA
   质量控制: 转化效率验证：合格；无菌检测：阴性；蓝白斑筛选功能：阳性
   储存与运输: -80°C或干冰运输与保存
   仅供科研使用

English:
BioVector® DH5α λpir Strain / Competent Cells Datasheet

Product Name: BioVector® DH5α λpir Competent Cells
Chinese Name: BioVector® DH5α λpir 大肠杆菌克隆菌株
English Name: BioVector® DH5α λpir E. coli Cloning Strain
Product Category: Competent Cells / Cloning Strain
Strain Type: E. coli (Escherichia coli)
Strain Application: Suitable for cloning and propagation of plasmids containing the R6K replication origin (ori), such as gene knockout vectors and suicide plasmids
Strain Genotype: F- endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG purB20 φ80dlacZΔM15 Δ(lacZYA-argF)U169 λ-
Characteristics:

1. λpir Lysogen: Expresses the π protein (product of the pir gene), which provides the essential replication protein in trans for plasmids carrying the R6K origin of replication, enabling stable replication and maintenance of such plasmids in this strain

2. recA1 and endA1: Lacks homologous recombination activity, improving stability of inserted DNA fragments; lacks non-specific endonuclease activity, improving yield and quality of plasmid DNA preparations

3. lacZΔM15: Allows blue-white screening with pUC series vectors
   Control Vector: BioVector® pR6K Plasmid (containing the R6K replication origin)
   Transformation Efficiency: ≥ 1 x 10⁷ cfu/µg pR6K plasmid DNA
   Quality Control: Transformation Efficiency Validation: Pass; Sterility: Negative; Blue-White Screening Function: Positive
   Storage and Shipment: Stored and shipped at -80°C or on dry ice
   For Research Use Only

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