UB/OC-1 BioVector®小鼠耳蜗毛细胞系 / Mouse Cochlear Hair Cell Line BioVector NTCC典型培养物保藏中心

BioVector® UB/OC-1 小鼠耳蜗毛细胞系 / Mouse Cochlear Hair Cell Line 数据说明书

产品名称: BioVector® UB/OC-1 细胞
同义词: BioVector® University of Bristol/Organ of Corti-1; BioVector® 耳蜗毛细胞系1号
中文名称: BioVector® UB/OC-1 小鼠耳蜗毛细胞系
英文名称: BioVector® UB/OC-1 Mouse Cochlear Hair Cell Line
产品类别: 感觉神经上皮细胞系
物种来源: 小鼠 (Mus musculus)，C57BL/6 背景 -9
组织来源: 胚胎第13天 (E13) 小鼠耳蜗，柯蒂氏器 (Organ of Corti) -9
细胞类型: 耳蜗毛细胞前体
构建方法: 从携带条件性永生化基因 (H-2Kb-tsA58) 的 Immortomouse™ 中建立 -9
形态特性: 上皮细胞样
生长特性: 贴壁生长
特性特征:

1. 条件永生化: 在33°C含γ-干扰素的条件下增殖 (永生化表型)；在39°C无γ-干扰素的条件下分化 (非永生化表型) -9。

2. 标志物表达: 分化后表达毛细胞特征性基因，包括转录因子 Brn3.1 (POU4F3)、α9 乙酰胆碱受体亚基，以及 fimbrin、myosin VI 和 VIIA -1-3-6。

3. 应用: 被广泛用于研究毛细胞分化、基因调控、电生理特性以及探索听觉毛细胞再生的治疗方法 -1-3-6。
   培养基: BioVector® UB/OC-1 细胞专用完全培养基（推荐基础成分：含 Earle's 盐的 MEM，添加10%胎牛血清、50 U/mL γ-干扰素和 L-谷氨酰胺）-9
   培养条件:永生化/增殖条件： 气相：95%空气，5%二氧化碳；温度：33°C。分化条件： 温度：39°C (不含γ-干扰素) -9。
   传代方法: 推荐使用 BioVector® 专用传代试剂按比例进行传代。
   冻存液: BioVector® 细胞冻存液
   质控载体: BioVector® pUC19 质粒
   宿主菌: BioVector® DH5α 化学感受态细胞
   质量控制: 支原体检测：阴性；无菌检测：阴性
   储存与运输: 液氮或干冰运输与保存
   生物安全等级: 1
   仅供科研使用

English:
BioVector® UB/OC-1 Mouse Cochlear Hair Cell Line Datasheet

Product Name: BioVector® UB/OC-1 Cells
Synonyms: BioVector® University of Bristol/Organ of Corti-1; BioVector® Organ of Corti cell line number 1 -9
Chinese Name: BioVector® UB/OC-1 小鼠耳蜗毛细胞系
English Name: BioVector® UB/OC-1 Mouse Cochlear Hair Cell Line
Product Category: Sensory Neuroepithelial Cell Line
Species of Origin: Mouse (Mus musculus), C57BL/6 background -9
Tissue of Origin: Embryonic day 13 (E13) mouse cochlea, Organ of Corti -9
Cell Type: Cochlear hair cell precursors
Derivation: Established from the Immortomouse™ harboring a conditional immortalizing gene (H-2Kb-tsA58) -9
Morphology: Epithelial-like
Growth Characteristics: Adherent
Characteristics:

1. Conditionally Immortalized: Proliferates at 33°C with interferon-gamma (immortalized phenotype); Differentiates at 39°C without interferon-gamma (non-immortalized phenotype) -9.

2. Marker Expression: Expresses hair cell-specific genes upon differentiation, including transcription factor Brn3.1 (POU4F3), the α9 acetylcholine receptor subunit, fimbrin, myosin VI, and myosin VIIA -1-3-6.

3. Applications: Widely used to study hair cell differentiation, gene regulation, electrophysiological properties, and to explore therapeutic methods for auditory hair cell regeneration -1-3-6.
   Culture Medium: BioVector® Complete Medium for UB/OC-1 Cells (Recommended composition: MEM with Earle's salts supplemented with 10% FBS, 50 U/mL interferon-gamma, and L-glutamine) -9
   Culture Conditions:Immortalizing/Proliferating Conditions: Atmosphere: 95% air, 5% CO₂; Temperature: 33°C. Differentiating Conditions: Temperature: 39°C (without interferon-gamma) -9.
   Subculturing Method: Recommended to use BioVector® Subculture Reagent at an appropriate split ratio.
   Freeze Medium: BioVector® Cell Freeze Medium
   Control Vector: BioVector® pUC19 Plasmid
   Host Strain: BioVector® DH5α Chemically Competent Cells
   Quality Control: Mycoplasma: Negative; Sterility: Negative
   Storage and Shipment: Stored and shipped in liquid nitrogen or on dry ice
   Biosafety Level: 1
   For Research Use Only

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