HEI-OC1 BioVector®小鼠耳蜗毛细胞系 / Mouse Cochlear Hair Cell Line BioVector NTCC典型培养物保藏中心

BioVector® HEI-OC1 小鼠耳蜗毛细胞系 / Mouse Cochlear Hair Cell Line 数据说明书

产品名称: BioVector® HEI-OC1 细胞
同义词: BioVector® HEIOC1; BioVector® 小鼠耳蜗毛细胞系
中文名称: BioVector® HEI-OC1 小鼠耳蜗毛细胞系
英文名称: BioVector® HEI-OC1 Mouse Cochlear Hair Cell Line
产品类别: 感觉神经上皮细胞系
物种来源: 小鼠 (Mus musculus)
组织来源: 耳蜗 (Cochlea)，柯蒂氏器 (Organ of Corti)
细胞类型: 耳蜗毛细胞前体/祖细胞
形态特性: 上皮细胞样，贴壁生长，呈典型的"铺路石"样排列 -4-7
生长特性: 贴壁生长

特性特征:

1. 温度敏感型: 在33°C含γ-干扰素条件下增殖迅速且去分化；在37°C无γ-干扰素条件下分化并表达毛细胞标志物 -4-7。

2. 标志物表达: 分化条件下表达毛细胞特异性标记，如转录因子Brn3.1 (POU4F3) 和肌球蛋白VIIA (Myosin VIIA) -2-7。

3. 应用广泛: 是国际公认的用于研究听觉毛细胞凋亡、自噬、衰老、氧化应激、炎症反应以及耳毒性药物筛选和听力保护机制的金标准体外模型 -3-4-8。
   培养基: BioVector® HEI-OC1 细胞专用完全培养基（基础成分为高糖DMEM培养基，添加10%胎牛血清和1%青霉素-链霉素）-3-5-9
   培养条件: 气相：95%空气，5%二氧化碳；温度：37°C (用于分化培养/常规实验) -3-4-7
   传代方法: 当细胞融合度达到80%-90%时，推荐使用 BioVector® 专用传代试剂（如0.25%胰蛋白酶-EDTA），按1:2至1:4比例进行传代，每2-3天换液一次 -4-5-9。
   冻存液: BioVector® 细胞冻存液（推荐配方：90%胎牛血清 + 10% DMSO）-3-9
   质控载体: BioVector® pUC19 质粒
   宿主菌: BioVector® DH5α 化学感受态细胞
   质量控制: 支原体检测：阴性；无菌检测：阴性；细菌检测：阴性 -5；STR鉴定：已通过 -9
   储存与运输: 液氮或干冰运输与保存
   生物安全等级: 1
   仅供科研使用

English:
BioVector® HEI-OC1 Mouse Cochlear Hair Cell Line Datasheet

Product Name: BioVector® HEI-OC1 Cells
Synonyms: BioVector® HEIOC1; BioVector® Mouse Cochlear Hair Cell Line
Chinese Name: BioVector® HEI-OC1 小鼠耳蜗毛细胞系
English Name: BioVector® HEI-OC1 Mouse Cochlear Hair Cell Line
Product Category: Sensory Neuroepithelial Cell Line
Species of Origin: Mouse (Mus musculus)
Tissue of Origin: Cochlea, Organ of Corti
Cell Type: Cochlear hair cell progenitors/precursors
Morphology: Epithelial-like, adherent, exhibiting a characteristic "cobblestone" monolayer arrangement -4-7
Growth Characteristics: Adherent
Characteristics:

1. Temperature-Sensitive: Proliferates and dedifferentiates rapidly at 33°C with interferon-gamma; Differentiates and expresses hair cell markers at 37°C without interferon-gamma -4-7.

2. Marker Expression: Expresses hair cell-specific markers upon differentiation, such as the transcription factor Brn3.1 (POU4F3) and Myosin VIIA -2-7.

3. Broad Applications: It is the gold-standard in vitro model internationally recognized for studying auditory hair cell apoptosis, autophagy, senescence, oxidative stress, inflammation, and for ototoxicity drug screening and hearing protection mechanism research -3-4-8.
   Culture Medium: BioVector® Complete Medium for HEI-OC1 Cells (Composition: High-glucose DMEM supplemented with 10% fetal bovine serum and 1% Penicillin-Streptomycin) -3-5-9
   Culture Conditions: Atmosphere: 95% air, 5% CO₂; Temperature: 37°C (for differentiation/standard experiments) -3-4-7
   Subculturing Method: When cells reach 80%-90% confluence, recommended to use BioVector® Subculture Reagent (e.g., 0.25% Trypsin-EDTA) at a split ratio of 1:2 to 1:4. Medium renewal is recommended every 2-3 days -4-5-9.
   Freeze Medium: BioVector® Cell Freeze Medium (Recommended composition: 90% FBS + 10% DMSO) -3-9
   Control Vector: BioVector® pUC19 Plasmid
   Host Strain: BioVector® DH5α Chemically Competent Cells
   Quality Control: Mycoplasma: Negative; Sterility: Negative; Bacterial: Negative -5; STR Profiling: Authenticated -9
   Storage and Shipment: Stored and shipped in liquid nitrogen or on dry ice
   Biosafety Level: 1
   For Research Use Only

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