hEM15A BioVector®人子宫内膜异位症患者在位内膜间质细胞永生化细胞系BioVector NTCC典型培养物保藏中心

BioVector® hEM15A 人子宫内膜异位症患者在位内膜间质细胞永生化细胞系 / Human Endometriosis-Derived Immortalized Eutopic Endometrium Stromal Cell Line 数据说明书

产品名称: BioVector® hEM15A 细胞
中文名称: BioVector® hEM15A 人子宫内膜异位症患者在位内膜间质细胞永生化细胞系
英文名称: BioVector® hEM15A Human Endometriosis-Derived Immortalized Eutopic Endometrium Stromal Cell Line
产品类别: 妇科疾病研究细胞系
物种来源: 人 (Homo sapiens)
组织来源: 子宫内膜（在位内膜）
疾病: 子宫内膜异位症
患者信息: 33岁女性
形态特性: 梭形，成纤维细胞样
生长特性: 贴壁生长
构建方法: 采用PCD2SV40T质粒转染人子宫内膜异位症患者在位内膜细胞，经G418筛选和有限稀释法克隆建立
特性特征:

1. 首次建立: 首个源自亚洲人子宫内膜异位症组织的永生化在位内膜间质细胞系

2. 核型: 正常二倍体核型，染色体结构和数量未见异常

3. 致瘤性: 裸鼠体内接种未观察到致瘤性

4. 标志物表达: 具有子宫内膜间质细胞特征；雌激素受体阳性，孕激素受体阳性

5. 传代能力: 可连续传代培养
   培养基: BioVector® hEM15A 细胞专用完全培养基（推荐基础成分：DMEM/F12 培养基，添加10-15% 胎牛血清和1% 青霉素-链霉素）
   培养条件: 气相：95%空气，5%二氧化碳；温度：37°C
   传代方法: 当细胞融合度达到80-90%时，推荐使用 BioVector® 专用传代试剂（如0.25%胰蛋白酶-EDTA）消化1-3分钟，按1:2至1:3比例进行传代，每2-3天换液一次
   冻存液: BioVector® 细胞冻存液
   质量控制: 支原体检测：阴性；无菌检测：阴性；STR分型：已验证
   STR分型数据: Amelogenin: X；CSF1PO: 10,11；D12S391: 21,22；D13S317: 8；D18S51: 15,17；D19S433: 13；D21S11: 29,32.2；D2S1338: 19,25；D3S1358: 16,17；D5S818: 10,11；D6S1043: 14,18；D7S820: 11,12；D8S1179: 10,13；FGA: 22,23；Penta E: 14,16；D16S539: 10,12；TH01: 9；TPOX: 8,11；vWA: 14,18
   储存与运输: 液氮或干冰运输与保存
   生物安全等级: 1
   仅供科研使用

English:
BioVector® hEM15A Human Endometriosis-Derived Immortalized Eutopic Endometrium Stromal Cell Line Datasheet

Product Name: BioVector® hEM15A Cells
Chinese Name: BioVector® hEM15A 人子宫内膜异位症患者在位内膜间质细胞永生化细胞系
English Name: BioVector® hEM15A Human Endometriosis-Derived Immortalized Eutopic Endometrium Stromal Cell Line
Product Category: Gynecological Disease Research Cell Line
Species of Origin: Human (Homo sapiens)
Tissue of Origin: Endometrium (eutopic endometrium)
Disease: Endometriosis
Patient Information: 33-year-old female
Morphology: Spindle-shaped, fibroblast-like
Growth Characteristics: Adherent
Derivation: Established by transfecting cells from eutopic endometrium of an endometriosis patient with the PCD2SV40T plasmid, followed by G418 selection and limited dilution cloning
Characteristics:

1. First of its Kind: The first immortalized eutopic endometrium stromal cell line derived from Asian endometriosis tissue

2. Karyotype: Normal diploid karyotype, with no abnormalities in chromosome structure or number observed

3. Tumorigenicity: No tumorigenicity observed upon implantation in nude mice

4. Marker Expression: Exhibits characteristics of endometrium stromal cells; Estrogen Receptor positive, Progesterone Receptor positive

5. Passaging Capacity: Can be passaged continuously
   Culture Medium: BioVector® Complete Medium for hEM15A Cells (Recommended composition: DMEM/F12 medium supplemented with 10-15% fetal bovine serum and 1% Penicillin-Streptomycin)
   Culture Conditions: Atmosphere: 95% air, 5% CO₂; Temperature: 37°C
   Subculturing Method: When cells reach 80-90% confluence, recommended to use BioVector® Subculture Reagent (e.g., 0.25% Trypsin-EDTA) for 1-3 minutes. Subculture at a split ratio of 1:2 to 1:3. Medium renewal is recommended every 2-3 days
   Freeze Medium: BioVector® Cell Freeze Medium
   Quality Control: Mycoplasma: Negative; Sterility: Negative; STR Profiling: Authenticated
   STR Profile Data: Amelogenin: X; CSF1PO: 10,11; D12S391: 21,22; D13S317: 8; D18S51: 15,17; D19S433: 13; D21S11: 29,32.2; D2S1338: 19,25; D3S1358: 16,17; D5S818: 10,11; D6S1043: 14,18; D7S820: 11,12; D8S1179: 10,13; FGA: 22,23; Penta E: 14,16; D16S539: 10,12; TH01: 9; TPOX: 8,11; vWA: 14,18
   Storage and Shipment: Stored and shipped in liquid nitrogen or on dry ice
   Biosafety Level: 1
   For Research Use Only

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

电话:400-800-2947

工作QQ/微信同号:1843439339

网址

http://www.biovector.net