FBCP-Mix BioVector®牛角膜细胞系 / FBCP-Mix Cell Line BioVector NTCC典型培养物保藏中心

BioVector® FBCP-Mix 细胞系 / FBCP-Mix Cell Line 数据说明书

产品名称: BioVector® FBCP-Mix 细胞
中文名称: BioVector® FBCP-Mix 混合细胞系
英文名称: BioVector® FBCP-Mix Mixed Cell Line
产品类别: 原代混合细胞培养物 / 组织工程研究模型
物种来源: 牛 (Bos taurus)
组织来源: 角膜 (Cornea)，可能包含角膜基质和内皮层细胞
细胞类型: 混合细胞群，包含成纤维细胞样细胞和上皮/内皮样细胞
形态特性: 混合形态，可见梭形成纤维细胞和多边形上皮样细胞
生长特性: 贴壁生长
构建方法: 从牛角膜组织分离消化获得原代细胞，经有限传代后混合培养，未进行克隆纯化，保留细胞异质性
特性特征:

1. 异质性: 包含多种细胞类型，模拟角膜组织的细胞多样性，适用于组织工程、细胞间相互作用研究

2. 增殖能力: 混合培养条件下细胞可维持一定增殖能力，但长期传代可能导致细胞比例改变

3. 应用: 常用于角膜创伤愈合模型、药物渗透研究、生物材料相容性测试等
   培养基: BioVector® FBCP-Mix 细胞专用完全培养基（推荐基础成分：DMEM/F12 (1:1) 培养基，添加10% 胎牛血清，1% 青霉素-链霉素，2mM L-谷氨酰胺，1% 非必需氨基酸）
   培养条件: 气相：95%空气，5%二氧化碳；温度：37°C
   传代方法: 当细胞融合度达到80-90%时，推荐使用 BioVector® 专用传代试剂（如0.25%胰蛋白酶-EDTA）消化，按1:2比例进行传代，每2-3天换液一次
   冻存液: BioVector® 细胞冻存液（推荐配方：90%胎牛血清 + 10% DMSO）
   质量控制: 支原体检测：阴性；无菌检测：阴性；细胞活力：复苏后活力>80%
   储存与运输: 液氮或干冰运输与保存
   生物安全等级: 1
   仅供科研使用

English:
BioVector® FBCP-Mix Cell Line Datasheet

Product Name: BioVector® FBCP-Mix Cells
Chinese Name: BioVector® FBCP-Mix 混合细胞系
English Name: BioVector® FBCP-Mix Mixed Cell Line
Product Category: Primary Mixed Cell Culture / Tissue Engineering Research Model
Species of Origin: Bovine (Bos taurus)
Tissue of Origin: Cornea, likely including stromal and endothelial layers
Cell Type: Mixed cell population, containing fibroblast-like cells and epithelial/endothelial-like cells
Morphology: Mixed morphology, with spindle-shaped fibroblasts and polygonal epithelial-like cells visible
Growth Characteristics: Adherent
Derivation: Primary cells isolated and digested from bovine corneal tissue, cultured after limited passages without clonal purification, retaining cellular heterogeneity
Characteristics:

1. Heterogeneity: Contains multiple cell types, mimicking the cellular diversity of corneal tissue, suitable for tissue engineering and cell-cell interaction studies

2. Proliferation Capacity: Maintains certain proliferative capacity under mixed culture conditions, but long-term passage may alter cell proportions

3. Applications: Commonly used in corneal wound healing models, drug permeability studies, biocompatibility testing of biomaterials, etc.
   Culture Medium: BioVector® Complete Medium for FBCP-Mix Cells (Recommended composition: DMEM/F12 (1:1) medium supplemented with 10% fetal bovine serum, 1% penicillin-streptomycin, 2mM L-glutamine, 1% non-essential amino acids)
   Culture Conditions: Atmosphere: 95% air, 5% CO₂; Temperature: 37°C
   Subculturing Method: When cells reach 80-90% confluence, recommended to use BioVector® Subculture Reagent (e.g., 0.25% Trypsin-EDTA). Subculture at a split ratio of 1:2. Medium renewal is recommended every 2-3 days
   Freeze Medium: BioVector® Cell Freeze Medium (Recommended composition: 90% FBS + 10% DMSO)
   Quality Control: Mycoplasma: Negative; Sterility: Negative; Cell Viability: >80% upon recovery
   Storage and Shipment: Stored and shipped in liquid nitrogen or on dry ice
   Biosafety Level: 1
   For Research Use Only

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