pINA1293 BioVector®解脂耶氏酵母表达载体质粒pINA1293 Plasmid BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

BioVector® pINA1293 质粒 / pINA1293 Plasmid 数据说明书

产品名称: BioVector® pINA1293 质粒
中文名称: BioVector® pINA1293 解脂耶氏酵母表达质粒
英文名称: BioVector® pINA1293 Yarrowia lipolytica Expression Plasmid
产品类别: 酵母表达载体
质粒类型: 穿梭质粒；解脂耶氏酵母表达载体
用途: 在解脂耶氏酵母中表达外源基因；蛋白生产；代谢工程研究
来源信息: 由法国农业科学研究院构建并保藏
载体大小: 约 8.9 kb
复制子: pBR322 ori（大肠杆菌中复制）
启动子: hp4d 启动子（组成型杂交启动子，来源于LEU2启动子和XPR2启动子的UAS1区）
终止子: XPR2 终止子
筛选标记:

• 大肠杆菌筛选：卡那霉素抗性 (Kanamycin)

• 酵母筛选：LEU2 营养缺陷型标记
  信号肽: 携带XPR2前原区信号肽，用于引导蛋白分泌
  多克隆位点: 位于启动子和终止子之间，提供多个限制性酶切位点
  特性特征:

1. 宿主范围: 可在大肠杆菌中扩增，在解脂耶氏酵母中表达

2. 表达特性: hp4d启动子为组成型启动子，无需诱导即可驱动外源基因表达

3. 分泌表达: 携带XPR2信号肽，可实现外源蛋白的分泌表达

4. 应用: 广泛用于解脂耶氏酵母中外源蛋白生产、代谢途径工程、工业酶生产等研究
   相关菌株: BioVector® 解脂耶氏酵母 Po1系列菌株 (Po1d, Po1g, Po1h) 等
   质控载体: BioVector® pUC19 质粒
   宿主菌: BioVector® DH5α 化学感受态细胞
   质量控制: 测序验证：100%正确；无菌检测：阴性；酶切鉴定：符合预期条带
   储存与运输: -20°C或干冰运输与保存
   仅供科研使用

English:
BioVector® pINA1293 Plasmid Datasheet

Product Name: BioVector® pINA1293 Plasmid
Chinese Name: BioVector® pINA1293 解脂耶氏酵母表达质粒
English Name: BioVector® pINA1293 Yarrowia lipolytica Expression Plasmid
Product Category: Yeast Expression Vector
Plasmid Type: Shuttle Plasmid; Yarrowia lipolytica Expression Vector
Purpose: Expression of foreign genes in Yarrowia lipolytica; protein production; metabolic engineering research
Source: Constructed and deposited by the French National Institute for Agricultural Research
Vector Size: Approximately 8.9 kb
Replicon: pBR322 ori (for propagation in E. coli)
Promoter: hp4d promoter (constitutive hybrid promoter derived from the LEU2 promoter and the UAS1 region of the XPR2 promoter)
Terminator: XPR2 terminator
Selection Markers:

• E. coli Selection: Kanamycin resistance

• Yeast Selection: LEU2 auxotrophic marker
  Signal Peptide: Contains the XPR2 pre-pro region signal peptide for protein secretion
  Multiple Cloning Site: Located between the promoter and terminator, providing multiple restriction enzyme sites
  Characteristics:

1. Host Range: Can be amplified in E. coli and expressed in Yarrowia lipolytica

2. Expression Characteristics: The hp4d promoter is constitutive, driving foreign gene expression without induction

3. Secretory Expression: Contains the XPR2 signal peptide, enabling secretory expression of foreign proteins

4. Applications: Widely used in foreign protein production in Yarrowia lipolytica, metabolic pathway engineering, industrial enzyme production research
   Related Strains: BioVector® Yarrowia lipolytica Po1 series strains (Po1d, Po1g, Po1h) etc.
   Control Vector: BioVector® pUC19 Plasmid
   Host Strain: BioVector® DH5α Chemically Competent Cells
   Quality Control: Sequencing Validation: 100% correct; Sterility: Negative; Restriction Digestion: Expected bands
   Storage and Shipment: Stored and shipped at -20°C or on dry ice
   For Research Use Only

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

电话:400-800-2947

工作QQ/微信同号:1843439339

网址http://www.biovector.net