pINA1292 BioVector®解脂耶氏酵母表达质粒 / pINA1292 Plasmid BioVector NTCC典型培养物保藏中心

BioVector® pINA1292解脂耶氏酵母表达质粒 / pINA1292 Plasmid 数据说明书

产品名称: BioVector® pINA1292 质粒
中文名称: BioVector® pINA1292 解脂耶氏酵母表达质粒（多拷贝整合型）
英文名称: BioVector® pINA1292 Yarrowia lipolytica Expression Plasmid (Multicopy Integrative Vector)
产品类别: 酵母表达载体
质粒类型: 穿梭质粒；多拷贝整合型解脂耶氏酵母表达载体
用途: 在解脂耶氏酵母中实现外源基因的多拷贝整合与高效表达；蛋白生产；代谢工程研究；基因功能研究 -2-7
来源信息: 由法国农业科学研究院构建并保藏 -4
载体大小: 相关信息缺失
复制子: pBR322 ori（在大肠杆菌中复制）-4
启动子: hp4d 启动子（组成型杂交启动子，来源于LEU2启动子和XPR2启动子的UAS1区）-4
终止子: XPR2 终止子 (XPR2t) -4
筛选标记:

• 大肠杆菌筛选：相关信息缺失

• 酵母筛选：URA3 营养缺陷型标记（携带ura3d4缺陷型等位基因，可实现多拷贝整合）-4-7
  信号肽: 无信号肽（用于胞内表达）-4
  多克隆位点: 上游酶切位点：PmlI (平末端)；下游酶切位点：BamHI, KpnI -4
  特性特征:

1. 宿主范围: 可在大肠杆菌中扩增，在解脂耶氏酵母中整合表达

2. 整合特性: 属于“自动克隆”载体系列，表达盒可通过NotI酶切释放，去除细菌序列后转化酵母；携带ura3d4缺陷型标记，可在解脂耶氏酵母中实现多拷贝整合 -4-7

3. 表达特性: hp4d启动子为组成型启动子，无需诱导即可驱动外源基因表达

4. 应用: 广泛用于解脂耶氏酵母中外源蛋白的高水平表达、代谢途径工程、工业酶生产等研究。该载体已成功用于过表达delta-12去饱和酶以生产亚油酸，并研究其细胞毒性效应 -2-7
   相关质粒: BioVector® pINA1312（单拷贝整合版本，携带ura3d1等位基因）；BioVector® pINA1293（携带LIP2信号肽的分泌表达版本）-4
   质控载体: BioVector® pUC19 质粒
   宿主菌: BioVector® DH5α 化学感受态细胞
   质量控制: 测序验证：100%正确；无菌检测：阴性；酶切鉴定：符合预期条带
   储存与运输: -20°C或干冰运输与保存
   仅供科研使用

English:
BioVector® pINA1292 Plasmid Datasheet

Product Name: BioVector® pINA1292 Plasmid
Chinese Name: BioVector® pINA1292 解脂耶氏酵母表达质粒（多拷贝整合型）
English Name: BioVector® pINA1292 Yarrowia lipolytica Expression Plasmid (Multicopy Integrative Vector)
Product Category: Yeast Expression Vector
Plasmid Type: Shuttle Plasmid; Multicopy Integrative Yarrowia lipolytica Expression Vector
Purpose: Multicopy integration and high-level expression of foreign genes in Yarrowia lipolytica; protein production; metabolic engineering research; gene function studies -2-7
Source: Constructed and deposited by the French National Institute for Agricultural Research -4
Vector Size: Information missing
Replicon: pBR322 ori (for propagation in E. coli) -4
Promoter: hp4d promoter (constitutive hybrid promoter derived from the LEU2 promoter and the UAS1 region of the XPR2 promoter) -4
Terminator: XPR2 terminator (XPR2t) -4
Selection Markers:

• E. coli Selection: Information missing

• Yeast Selection: URA3 auxotrophic marker (carries the ura3d4 defective allele for multicopy integration) -4-7
  Signal Peptide: None (for intracellular expression) -4
  Multiple Cloning Site: Upstream site: PmlI (blunt end); Downstream sites: BamHI, KpnI -4
  Characteristics:

1. Host Range: Can be amplified in E. coli and integrated for expression in Yarrowia lipolytica

2. Integration Properties: Belongs to the "auto-cloning" vector series; the expression cassette can be released by NotI digestion to remove bacterial sequences before transformation; carries the ura3d4 defective marker enabling multicopy integration in Yarrowia lipolytica -4-7

3. Expression Characteristics: The hp4d promoter is constitutive, driving foreign gene expression without induction

4. Applications: Widely used for high-level expression of foreign proteins in Yarrowia lipolytica, metabolic pathway engineering, industrial enzyme production research. This vector has been successfully used to overexpress delta-12 desaturase for linoleic acid production and to study its cytotoxic effects -2-7
   Related Plasmids: BioVector® pINA1312 (single-copy integration version carrying ura3d1 allele); BioVector® pINA1293 (secretory expression version with LIP2 signal peptide) -4
   Control Vector: BioVector® pUC19 Plasmid
   Host Strain: BioVector® DH5α Chemically Competent Cells
   Quality Control: Sequencing Validation: 100% correct; Sterility: Negative; Restriction Digestion: Expected bands
   Storage and Shipment: Stored and shipped at -20°C or on dry ice
   For Research Use Only

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

电话:400-800-2947

工作QQ/微信同号:1843439339

网址

http://www.biovector.net