EHSPEL BioVector®小鼠parietal内胚层样细胞系 / Mouse Parietal Endoderm-Like Cell Line

BioVector® EHSPEL 小鼠 parietal 内胚层样细胞系 / Mouse Parietal Endoderm-Like Cell Line 数据说明书

产品名称: BioVector® EHSPEL 细胞
同义词: BioVector® EHS Parietal Endoderm-Like; BioVector® Engelbreth-Holm-Swarm Parietal Endoderm-Like
中文名称: BioVector® EHSPEL 小鼠 parietal 内胚层样细胞系
英文名称: BioVector® EHSPEL Mouse Parietal Endoderm-Like Cell Line
产品类别: 肿瘤细胞系；基底膜基质研究模型
物种来源: 小鼠 (Mus musculus)，ST/Eh 品系
组织来源: Engelbreth-Holm-Swarm 肿瘤
疾病: 小鼠软骨肉瘤
细胞类型: parietal 内胚层样细胞
形态特性: 上皮样细胞形态
生长特性: 贴壁生长
构建方法: 从 Engelbreth-Holm-Swarm 肿瘤中分离建立，该肿瘤来源于 ST/Eh 品系小鼠的自发性肿瘤，通过移植传代维持，本细胞系为可在体外培养的细胞系

特性特征:

1. 基底膜组分分泌: 细胞向培养基中分泌大量 laminin-1，并在培养皿上沉积基底膜组分

2. 体内成瘤能力: 保留在体内形成肿瘤的能力，且肿瘤可积累大量基底膜组分

3. 基因表达谱: 与胚胎第13.5天 Reichert's 膜的 parietal 内胚层细胞、分化的 F9 胚胎癌细胞及 PYS-2 parietal 内胚层细胞相比，具有共同的特征性基因表达模式，同时保留部分生殖细胞样特征

4. 核型特征: 采用染色体涂染探针分析显示存在大量染色体间重排及部分染色体超二倍体

5. 基因工程应用: 可导入外源基因（如人 laminin-α4-EGFP 融合蛋白），产生并沉积人-鼠嵌合 laminin-8，适用于构建嵌合 laminin 及具有修饰生物活性的基底膜样凝胶的高效生产系统
   研究应用:

6. 基底膜基质工程与生产

7. 基底膜组分分泌机制研究

8. 嵌合 laminin 及修饰基底膜基质的生产系统开发

9. 肿瘤微环境与基底膜相互作用研究
   培养基: BioVector® EHSPEL 细胞专用完全培养基（推荐基础成分：DMEM 高糖培养基，添加10%胎牛血清和1%青霉素-链霉素）
   培养条件: 气相：95%空气，5%二氧化碳；温度：37°C
   传代方法: 当细胞融合度达到80-90%时，推荐使用 BioVector® 专用传代试剂（如0.25%胰蛋白酶-EDTA）进行消化，按1:3至1:6比例进行传代
   冻存液: BioVector® 细胞冻存液（推荐配方：90%胎牛血清 + 10% DMSO）
   质量控制: 支原体检测：阴性；无菌检测：阴性；基底膜组分分泌验证：阳性
   储存与运输: 液氮或干冰运输与保存
   生物安全等级: 1
   仅供科研使用

English:
BioVector® EHSPEL Mouse Parietal Endoderm-Like Cell Line Datasheet

Product Name: BioVector® EHSPEL Cells
Synonyms: BioVector® EHS Parietal Endoderm-Like; BioVector® Engelbreth-Holm-Swarm Parietal Endoderm-Like
Chinese Name: BioVector® EHSPEL 小鼠 parietal 内胚层样细胞系
English Name: BioVector® EHSPEL Mouse Parietal Endoderm-Like Cell Line
Product Category: Cancer Cell Line; Basement Membrane Matrix Research Model
Species of Origin: Mouse (Mus musculus), ST/Eh strain
Tissue of Origin: Engelbreth-Holm-Swarm tumor
Disease: Mouse chondrosarcoma
Cell Type: Parietal endoderm-like cells
Morphology: Epithelial-like morphology
Growth Characteristics: Adherent
Derivation: Established from Engelbreth-Holm-Swarm tumor, which arose spontaneously in an ST/Eh strain mouse and has been propagated by transplantation. This cell line can be cultured ex vivo
Characteristics:

1. Basement Membrane Component Secretion: Secretes large amounts of laminin-1 into the culture medium and deposits basement membrane components onto dishes

2. In Vivo Tumor Formation: Retains the capacity to form tumors in vivo, with tumors accumulating abundant basement membrane components

3. Gene Expression Profile: Gene expression profile shares common features with parietal endoderm cells from Reichert's membrane at embryonic day 13.5, differentiated F9 embryonal carcinoma cells, and PYS-2 parietal endoderm cells, while also retaining germline cell-like characteristics

4. Karyotype: Chromosome painting analysis reveals a large number of interchromosomal rearrangements and partial chromosome hyperploidy

5. Genetic Engineering Applications: Can be used to introduce exogenous genes (e.g., human laminin-α4-EGFP fusion protein), resulting in production and deposition of human-mouse hybrid laminin-8; applicable for developing efficient systems to produce chimeric laminins and basement membrane-like gels with modified biological activity
   Research Applications:

6. Basement membrane matrix engineering and production

7. Mechanisms of basement membrane component secretion

8. Development of production systems for chimeric laminins and modified basement membrane matrices

9. Tumor microenvironment and basement membrane interaction studies
   Culture Medium: BioVector® Complete Medium for EHSPEL Cells (Recommended composition: DMEM high glucose medium supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin)
   Culture Conditions: Atmosphere: 95% air, 5% CO₂; Temperature: 37°C
   Subculturing Method: When cells reach 80-90% confluence, recommended to use BioVector® Subculture Reagent (e.g., 0.25% Trypsin-EDTA). Subculture at a split ratio of 1:3 to 1:6
   Freeze Medium: BioVector® Cell Freeze Medium (Recommended composition: 90% FBS + 10% DMSO)
   Quality Control: Mycoplasma: Negative; Sterility: Negative; Basement Membrane Component Secretion Validation: Positive
   Storage and Shipment: Stored and shipped in liquid nitrogen or on dry ice
   Biosafety Level: 1
   For Research Use Only

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