PAEAG1 BioVector®多重耐药铜绿假单胞菌株Pseudomonas aeruginosa PAEAG1 Strain BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

BioVector® 铜绿假单胞菌 PAEAG1 菌株 / Pseudomonas aeruginosa PAEAG1 Strain 数据说明书

产品名称: BioVector® 铜绿假单胞菌 PAEAG1 菌株
同义词: BioVector® P. aeruginosa AG1; BioVector® 多重耐药铜绿假单胞菌菌株
中文名称: BioVector® PAEAG1 多重耐药铜绿假单胞菌菌株
英文名称: BioVector® Pseudomonas aeruginosa PAEAG1 Multi-Drug Resistant Strain
产品类别: 多重耐药菌株；高危克隆菌株
菌株类型: 铜绿假单胞菌 (Pseudomonas aeruginosa)
菌株分型: ST-111 高危克隆型
分离来源: 2010年分离自哥斯达黎加一家大型医院患者的临床样本
保藏信息: 由哥斯达黎加大学 Fernando García 课题组分离保藏
耐药表型: 对β-内酰胺类抗生素（包括碳青霉烯类）、氨基糖苷类和氟喹诺酮类等多类抗菌药物同时耐药

特性特征:

1. 双重耐药特征: 同时对β-内酰胺类和氟喹诺酮类抗生素产生耐药性，属于多重耐药（MDR）菌株-3

2. 金属β-内酰胺酶: 首次报道同时携带 VIM-2 和 IMP-18 基因编码的金属β-内酰胺酶（MBL），这两种酶均具有碳青霉烯酶活性，可水解碳青霉烯类抗生素-3

3. 基因组特征: 基因组包含 57 个基因组岛，包括 6 个原噬菌体和 2 个完整的带有 MBLs 基因的整合子；预测有 250 个基因与毒力相关，60 个基因与耐药性相关-3

4. 氟喹诺酮耐药机制: 环丙沙星处理后，518 个基因呈现差异表达，包括噬菌体基因上调-3

5. 临床分级: 由于对碳青霉烯类耐药，被世界卫生组织（WHO）列入耐药菌优先性列表的 Priority 1（关键优先级）-3

6. 风险特征: 作为高危克隆，具有征服医院环境的能力，可在医疗环境中持续存在并引起医院感染-3

分子耐药机制:

1. β-内酰胺类耐药:

   • 产金属β-内酰胺酶（VIM-2 和 IMP-18）-3

   • 染色体 AmpC 头孢菌素酶高表达-2

   • 外膜孔蛋白 OprD 缺失导致碳青霉烯类耐药-5

   • MexAB-OprM 等外排泵高表达-5

2. 氟喹诺酮类耐药:

   • DNA 旋转酶（GyrA）和拓扑异构酶 IV（ParC）靶位点突变-2

   • 质粒介导的喹诺酮耐药（PMQR）基因（如 qnrA、qnrB、acc(6')-Ib-cr 等）-8

   • MexEF-OprN 外排泵高表达-5-6

3. 协同耐药:

   • 同一菌株中多种耐药机制共存，PMQR 与 ESBL 基因高度相关-8

   • 质粒介导的耐药基因可水平传播-8

研究应用:

1. 多重耐药机制研究

2. 新型抗生素（如头孢他啶-阿维巴坦、头孢洛扎-他唑巴坦）的敏感性评估-5

3. 噬菌体疗法研究-7

4. 耐药性进化与传播机制研究

5. 高危克隆株的流行病学监测

6. 联合用药策略研究

培养基: BioVector® 铜绿假单胞菌专用培养基（推荐基础成分：LB 培养基、Mueller-Hinton 琼脂或胰蛋白胨大豆琼脂）
培养条件: 气相：95%空气，5%二氧化碳（可选）；温度：35-37°C；好氧培养
保存方法: -80°C 甘油保存或液氮长期保存
冻存保护剂: 20% 甘油 或 10% DMSO
产品形式: 冻干粉 / 甘油菌 / 斜面培养物
生物安全等级: 2（需在二级生物安全柜内操作）
质量控制: 无菌检测：阴性；活力检测：复苏后生长良好；耐药表型验证：符合描述
储存与运输: 4-10°C 冷藏运输（短期），干冰或液氮运输（长期）
仅供科研使用

English:
BioVector® Pseudomonas aeruginosa PAEAG1 Multi-Drug Resistant Strain Datasheet

Product Name: BioVector® Pseudomonas aeruginosa PAEAG1 Strain
Synonyms: BioVector® P. aeruginosa AG1; BioVector® Multi-Drug Resistant Pseudomonas aeruginosa Strain
Chinese Name: BioVector® PAEAG1 多重耐药铜绿假单胞菌菌株
English Name: BioVector® Pseudomonas aeruginosa PAEAG1 Multi-Drug Resistant Strain
Product Category: Multi-Drug Resistant Strain; High-Risk Clone Strain
Strain Type: Pseudomonas aeruginosa
Strain Typing: ST-111 high-risk clonal type
Isolation Source: Isolated from clinical samples of patients at a major hospital in Costa Rica in 2010
Deposit Information: Isolated and deposited by the Fernando García Laboratory, University of Costa Rica
Resistance Phenotype: Resistant to multiple classes of antibiotics including beta-lactams (including carbapenems), aminoglycosides, and fluoroquinolones

Characteristics:

1. Dual Resistance Profile: Exhibits simultaneous resistance to beta-lactam and fluoroquinolone antibiotics; classified as a multi-drug resistant (MDR) strain-3

2. Metallo-beta-lactamases: First report of a P. aeruginosa isolate carrying both VIM-2 and IMP-18 genes encoding metallo-beta-lactamases (MBL), both with carbapenemase activity-3

3. Genomic Features: Genome contains 57 genomic islands, including 6 prophages and 2 complete integrons carrying MBL genes; 250 genes predicted to be associated with virulence and 60 genes associated with antibiotic resistance-3

4. Fluoroquinolone Resistance Mechanism: 518 genes show differential expression upon ciprofloxacin treatment, including upregulation of phage genes-3

5. Clinical Classification: Listed as Priority 1 (Critical Priority) by the World Health Organization (WHO) due to carbapenem resistance-3

6. Risk Characteristics: As a high-risk clone, capable of colonizing hospital environments and causing nosocomial infections-3

Molecular Resistance Mechanisms:

1. Beta-Lactam Resistance:

   • Production of metallo-beta-lactamases (VIM-2 and IMP-18)-3

   • Overexpression of chromosomal AmpC cephalosporinase-2

   • Loss of outer membrane porin OprD leading to carbapenem resistance-5

   • Overexpression of efflux pumps such as MexAB-OprM-5

2. Fluoroquinolone Resistance:

   • Target site mutations in DNA gyrase (GyrA) and topoisomerase IV (ParC)-2

   • Plasmid-mediated quinolone resistance (PMQR) genes (e.g., qnrA, qnrB, acc(6')-Ib-cr)-8

   • Overexpression of MexEF-OprN efflux pump-5-6

3. Synergistic Resistance:

   • Coexistence of multiple resistance mechanisms within the same strain; high correlation between PMQR and ESBL genes-8

   • Plasmid-mediated resistance genes can be horizontally transferred-8

Research Applications:

1. Multi-drug resistance mechanism studies

2. Susceptibility evaluation of novel antibiotics (e.g., ceftazidime-avibactam, ceftolozane-tazobactam)-5

3. Phage therapy research-7

4. Studies on resistance evolution and dissemination mechanisms

5. Epidemiological surveillance of high-risk clones

6. Combination therapy strategy development

Culture Medium: BioVector® Pseudomonas aeruginosa Culture Medium (Recommended composition: LB medium, Mueller-Hinton agar, or trypticase soy agar)
Culture Conditions: Atmosphere: 95% air, 5% CO₂ (optional); Temperature: 35-37°C; Aerobic
Storage Methods: -80°C glycerol stock or liquid nitrogen long-term storage
Cryoprotectant: 20% glycerol or 10% DMSO
Product Format: Freeze-dried powder / Glycerol stock / Slant culture
Biosafety Level: 2 (Operate in a Class II biological safety cabinet)
Quality Control: Sterility: Negative; Viability: Good growth upon revival; Resistance Phenotype Validation: Confirms description
Storage and Shipment: Refrigerated transport at 4-10°C (short-term), dry ice or liquid nitrogen transport (long-term)
For Research Use Only

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