CCUG 58541 BioVector®大肠杆菌菌株 / Escherichia coli Strain BioVector NTCC典型培养物保藏中心

BioVector® CCUG 58541 大肠杆菌菌株 / Escherichia coli Strain 数据说明书

产品名称: BioVector® CCUG 58541 菌株
同义词: BioVector® SMI Ec1027; BioVector® 8623
中文名称: BioVector® CCUG 58541 大肠杆菌多重耐药菌株
英文名称: BioVector® CCUG 58541 Multi-Drug Resistant Escherichia coli Strain
产品类别: 多重耐药菌株；临床参考菌株
菌株类型: 大肠杆菌 (Escherichia coli)
序列分型: ST297 (Warwick) / ST666 (Pasteur)
保藏编号: CCUG 58541
保藏机构: 瑞典哥德堡大学菌种保藏中心 (Culture Collection, University of Gothenburg)
保藏信息: 由瑞典公共卫生局 B. Olsson-Liljequist 于 2009 年 8 月 21 日保藏 -2
鉴定方法: API ID32E -2

耐药表型:
对 β-内酰胺类抗生素（包括广谱头孢菌素和碳青霉烯类）、氟喹诺酮类、氨基糖苷类、甲氧苄啶等多类抗菌药物同时耐药 -2

分子耐药机制:

1. β-内酰胺类耐药:

   • 超广谱 β-内酰胺酶 (ESBL): 携带 CTX-M-14 β-内酰胺酶基因，可水解头孢噻肟、头孢曲松等广谱头孢菌素 -2

   • 碳青霉烯酶: 携带 OXA-48 型碳青霉烯酶基因，可水解厄他培南、美罗培南等碳青霉烯类抗生素

   • 遗传支持: OXA-48 基因位于 IncL 型质粒上，可水平传播

2. 氟喹诺酮类耐药:

   • 对环丙沙星高度耐药

   • 机制涉及靶位点突变（GyrA、ParC）及质粒介导的喹诺酮耐药基因

3. 氨基糖苷类耐药:

   • 对氨基糖苷类药物耐药 -2

4. 其他耐药:

   • 对甲氧苄啶耐药 -2

抗生素最低抑菌浓度 (MIC):

生理生化特征:

1. 生长条件: 需氧菌，37°C 生长良好 -1-3

2. 氧耐受: 好氧 -1

3. API ID32E 生化谱: 515 115 515 155 515 515 115 551 115 111 11 10 -2

4. 酶活性:

   • β-半乳糖苷酶: 阳性 -4

   • β-葡萄糖醛酸酶: 阳性 -4

   • 赖氨酸脱羧酶: 阳性 -4

   • 鸟氨酸脱羧酶: 阳性 -4

   • 精氨酸双水解酶: 阴性 -4

   • 脲酶: 阴性 -4

5. 代谢特征:

   • 吲哚产生: 阳性 -4

   • D-葡萄糖产酸: 阳性 -4

   • D-甘露醇产酸: 阳性 -4

   • 海藻糖产酸: 阳性 -4

   • L-阿拉伯糖产酸: 阳性 -4

   • 蔗糖产酸: 阴性 -4

   • 山梨醇产酸: 阴性 -4

特性特征:

1. 多重耐药性: 同时对碳青霉烯类、广谱头孢菌素、氟喹诺酮类和氨基糖苷类等多种重要临床抗生素耐药 -2

2. OXA-48 阳性: 携带 OXA-48 型碳青霉烯酶，该酶是国际范围内流行的主要碳青霉烯酶类型之一

3. ESBL 阳性: 携带 CTX-M-14 型超广谱 β-内酰胺酶，可水解广谱头孢菌素 -2

4. 质粒介导耐药: OXA-48 基因位于可水平转移的 IncL 型质粒上，具有传播风险

5. 高危克隆: 属于 ST666 型，是 OXA-48 型大肠杆菌的常见流行克隆型

6. 临床分级: 作为碳青霉烯类耐药肠杆菌科细菌（CRE），被世界卫生组织列为关键优先级耐药菌

研究应用:

1. 碳青霉烯类耐药机制研究

2. 超广谱 β-内酰胺酶与碳青霉烯酶共表达机制研究

3. 新型抗生素（头孢他啶-阿维巴坦、美罗培南-韦博巴坦等）的敏感性评估

4. 质粒介导耐药基因水平传播机制研究

5. 联合用药策略研究

6. 临床微生物实验室诊断方法学评价

7. 医院感染控制与耐药菌监测研究

培养基: BioVector® 大肠杆菌专用培养基（推荐基础成分：LB 培养基、Mueller-Hinton 琼脂、胰蛋白胨大豆琼脂或血琼脂）
培养条件: 气相：95% 空气，5% 二氧化碳（可选）；温度：35-37°C；好氧培养 -1
保存方法: -80°C 甘油保存或液氮长期保存
冻存保护剂: 20% 甘油 或 10% DMSO
产品形式: 冻干粉 / 甘油菌 / 斜面培养物
生物安全等级: 2（需在二级生物安全柜内操作）
质量控制: 无菌检测：阴性；活力检测：复苏后生长良好；耐药表型验证：符合描述
储存与运输: 4-10°C 冷藏运输（短期），干冰或液氮运输（长期）
相关菌株: BioVector® SMI Ec1027（同菌株不同编号）-2
仅供科研使用

English:
BioVector® CCUG 58541 Escherichia coli Strain Datasheet

Product Name: BioVector® CCUG 58541 Strain
Synonyms: BioVector® SMI Ec1027; BioVector® 8623
Chinese Name: BioVector® CCUG 58541 大肠杆菌多重耐药菌株
English Name: BioVector® CCUG 58541 Multi-Drug Resistant Escherichia coli Strain
Product Category: Multi-Drug Resistant Strain; Clinical Reference Strain
Strain Type: Escherichia coli
Sequence Type: ST297 (Warwick) / ST666 (Pasteur)
Deposit Number: CCUG 58541
Depositor Institution: Culture Collection, University of Gothenburg, Sweden
Deposit Information: Deposited by B. Olsson-Liljequist, Public Health Agency of Sweden, on August 21, 2009 -2
Identification Method: API ID32E -2

Resistance Phenotype:
Resistant to multiple classes of antibiotics including β-lactams (including broad-spectrum cephalosporins and carbapenems), fluoroquinolones, aminoglycosides, and trimethoprim -2

Molecular Resistance Mechanisms:

1. β-Lactam Resistance:

   • Extended-Spectrum β-Lactamase (ESBL): Carries CTX-M-14 β-lactamase gene, hydrolyzing broad-spectrum cephalosporins such as cefotaxime and ceftriaxone -2

   • Carbapenemase: Carries OXA-48 carbapenemase gene, hydrolyzing carbapenems such as ertapenem and meropenem

   • Genetic Support: OXA-48 gene located on an IncL-type plasmid, capable of horizontal transfer

2. Fluoroquinolone Resistance:

   • Highly resistant to ciprofloxacin

   • Mechanisms involve target site mutations (GyrA, ParC) and plasmid-mediated quinolone resistance genes

3. Aminoglycoside Resistance:

   • Resistant to aminoglycosides -2

4. Other Resistance:

   • Resistant to trimethoprim -2

Minimum Inhibitory Concentrations (MICs):

Physiological and Biochemical Characteristics:

1. Growth Conditions: Aerobe, grows well at 37°C -1-3

2. Oxygen Tolerance: Aerobic -1

3. API ID32E Profile: 515 115 515 155 515 515 115 551 115 111 11 10 -2

4. Enzyme Activities:

   • β-Galactosidase: Positive -4

   • β-Glucuronidase: Positive -4

   • Lysine decarboxylase: Positive -4

   • Ornithine decarboxylase: Positive -4

   • Arginine dihydrolase: Negative -4

   • Urease: Negative -4

5. Metabolic Characteristics:

   • Indole production: Positive -4

   • D-Glucose acid production: Positive -4

   • D-Mannitol acid production: Positive -4

   • Trehalose acid production: Positive -4

   • L-Arabinose acid production: Positive -4

   • Sucrose acid production: Negative -4

   • Sorbitol acid production: Negative -4

Characteristics:

1. Multi-Drug Resistance: Simultaneously resistant to multiple important clinical antibiotics including carbapenems, broad-spectrum cephalosporins, fluoroquinolones, and aminoglycosides -2

2. OXA-48 Positive: Carries OXA-48 carbapenemase, one of the globally prevalent carbapenemase types

3. ESBL Positive: Carries CTX-M-14 extended-spectrum β-lactamase, hydrolyzing broad-spectrum cephalosporins -2

4. Plasmid-Mediated Resistance: OXA-48 gene located on a horizontally transferable IncL-type plasmid, posing transmission risks

5. High-Risk Clone: Belongs to ST666 type, a common epidemic clonal type for OXA-48-positive E. coli

6. Clinical Classification: Classified as a carbapenem-resistant Enterobacteriaceae (CRE), listed by WHO as a critical priority resistant pathogen

Research Applications:

1. Carbapenem resistance mechanism studies

2. Co-expression mechanisms of ESBL and carbapenemase

3. Susceptibility evaluation of novel antibiotics (ceftazidime-avibactam, meropenem-vaborbactam, etc.)

4. Horizontal transfer mechanism studies of plasmid-mediated resistance genes

5. Combination therapy strategy development

6. Clinical microbiology laboratory diagnostic method evaluation

7. Hospital infection control and resistant bacteria surveillance studies

Culture Medium: BioVector® E. coli Culture Medium (Recommended composition: LB medium, Mueller-Hinton agar, trypticase soy agar, or blood agar)
Culture Conditions: Atmosphere: 95% air, 5% CO₂ (optional); Temperature: 35-37°C; Aerobic -1
Storage Methods: -80°C glycerol stock or liquid nitrogen long-term storage
Cryoprotectant: 20% glycerol or 10% DMSO
Product Format: Freeze-dried powder / Glycerol stock / Slant culture
Biosafety Level: 2 (Operate in a Class II biological safety cabinet)
Quality Control: Sterility: Negative; Viability: Good growth upon revival; Resistance Phenotype Validation: Confirms description
Storage and Shipment: Refrigerated transport at 4-10°C (short-term), dry ice or liquid nitrogen transport (long-term)
Related Strain: BioVector® SMI Ec1027 (same strain under different designation) -2
For Research Use Only

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