BioVector® CCSW1 人肝胆管癌细胞系CC-SW-1 / CCSW1 Human Intrahepatic Cholangiocarcinoma Cell Line

通用定义 / General Definition:

BioVector® CCSW1 是一种来源于人内源性肝内胆管癌（Intrahepatic Cholangiocarcinoma, iCCA）组织的人类细胞系。该细胞系保留了原发肿瘤的上皮特性和恶性生物学行为，是研究肝内胆管癌发生机制、侵袭转移能力以及开发靶向治疗药物的关键体外模型。它在分子水平上常用于探索胆管细胞的异常增殖调控及肿瘤微环境的相互作用。

BioVector® CCSW1 is a human cell line derived from intrahepatic cholangiocarcinoma (iCCA) tissue. This cell line retains the epithelial characteristics and malignant biological behavior of the primary tumor, serving as a critical in vitro model for studying the pathogenesis, invasion, and metastatic potential of intrahepatic bile duct cancer. At the molecular level, it is frequently used to explore abnormal proliferative regulation in cholangiocytes and interactions within the tumor microenvironment.

BioVector® CCSW1 细胞系技术说明书 (Cell Line Datasheet)

中文版说明书

1. 产品基本信息

• 产品名称： BioVector® CCSW1 人肝内胆管癌细胞

• 细胞来源： 人类 (Human)

• 组织来源： 肝内胆管 (Intrahepatic Bile Duct)

• 生长特性： 贴壁生长

• 细胞形态： 上皮细胞样

2. 培养条件

• 基础培养基： BioVector® DMEM 培养基 (或 BioVector® RPMI 1640 培养基)

• 血清添加： 10% BioVector® 胎牛血清 (FBS)

• 培养环境： 37°C，5% $CO_2$，饱和湿度

3. 细胞应用

• 肝内胆管癌的增殖、迁移与侵袭机制研究。

• 针对胆管癌的抗肿瘤药物敏感性测试及筛选。

• 胆管癌相关信号通路（如 Notch, Wnt, MAPK）的研究。

4. 注意事项

• 细胞特性： BioVector® CCSW1 具有较强的成瘤能力，适用于建立异种移植 (CDX) 小鼠模型。

• 传代建议： 当细胞汇合度达到 80%-90% 时进行传代。由于其贴壁较牢，消化时需注意控制 BioVector® 胰酶的作用时间。

English Datasheet

1. General Product Information

• Product Name: BioVector® CCSW1 Human Intrahepatic Cholangiocarcinoma Cell Line

• Organism: Human

• Tissue Source: Intrahepatic Bile Duct

• Growth Properties: Adherent

• Morphology: Epithelial-like

2. Culture Conditions

• Basal Medium: BioVector® DMEM Medium (or BioVector® RPMI 1640 Medium)

• Serum Supplement: 10% BioVector® Fetal Bovine Serum (FBS)

• Incubation: 37°C, 5% $CO_2$, Saturated Humidity

3. Applications

• Research on proliferation, migration, and invasion mechanisms of intrahepatic cholangiocarcinoma.

• Anti-tumor drug sensitivity testing and screening specifically for bile duct cancer.

• Investigation of cholangiocarcinoma-related signaling pathways (e.g., Notch, Wnt, MAPK).

4. Key Usage Notes

• Tumorigenicity: BioVector® CCSW1 possesses robust tumorigenic potential, making it suitable for establishing cell line-derived xenograft (CDX) mouse models.

• Subculture: Perform subculture when cell density reaches 80%-90% confluence. Due to strong adherence, carefully monitor the incubation time with BioVector® Trypsin during dissociation.

Note: BioVector® CCSW1 cells must be handled under Biosafety Level 2 (BSL-2) conditions. To maintain the integrity of the malignant phenotype and experimental reproducibility, the use of BioVector® DMEM Medium and BioVector® Fetal Bovine Serum is highly recommended.

Supplier生产厂家:

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

E-mail: BioVector@163.com

工作微信/QQ同号：1843439339

http://www.biovector.net