BioVector® mHypoE-38 小鼠下丘脑永生化神经元细胞系 / mHypoE-38 Mouse Immortalized Hypothalamic Neuronal Cell Line

通用定义 / General Definition:

BioVector® mHypoE-38 是一种源自胚胎期（E15、E17、E18）小鼠下丘脑组织的永生化细胞系。该细胞系通过逆转录病毒介导的 SV40 大 T 抗原转导技术建立。mHypoE-38 属于“胚胎源性下丘脑细胞系（Cellutions）”系列，保留了下丘脑神经元的许多生理特征。它在体外表达特定的神经肽和受体，是研究中枢神经系统对能量代谢、内分泌调控及神经分泌信号转导的理想细胞模型。

BioVector® mHypoE-38 is an immortalized neuronal cell line derived from embryonic mouse hypothalamic tissue (E15, E17, or E18). This line was established using retroviral-mediated transfer of the SV40 large T-antigen. Part of the "Embryonic Hypothalamic Cell Line" series, BioVector® mHypoE-38 retains many physiological characteristics of hypothalamic neurons. It expresses specific neuropeptides and receptors in vitro, serving as an ideal cellular model for studying the central nervous system's role in energy metabolism, endocrine regulation, and neurosecretory signaling pathways.

BioVector® mHypoE-38 细胞系技术说明书 (Cell Line Datasheet)

中文版说明书

1. 产品基本信息

• 产品名称： BioVector® mHypoE-38 小鼠下丘脑永生化神经元细胞

• 细胞来源： 小鼠 (C57BL/6)

• 组织来源： 下丘脑 (Hypothalamus)

• 生长特性： 贴壁生长

• 细胞形态： 神经元样，具有突起结构

2. 培养条件

• 基础培养基： BioVector® DMEM 培养基 (含 4.5 g/L 葡萄糖)

• 血清添加： 10% BioVector® 胎牛血清 (FBS)

• 培养环境： 37°C，5% $CO_2$，饱和湿度

3. 细胞应用

• 神经内分泌研究： 研究瘦素 (Leptin)、胰岛素或其他代谢激素对下丘脑神经元的信号传导。

• 昼夜节律研究： 探索下丘脑时钟基因的表达调控。

• 神经肽分泌： 检测特定神经肽 (如 NPY, AgRP 等) 的表达与调节。

4. 注意事项

• 细胞汇合度： 建议在细胞达到 80%-90% 汇合度时进行传代，避免过度生长导致神经突起交织过多而难以消化。

• 特性验证： 随代数增加，建议定期检测特定神经标记物或激素受体的表达，以确保其生理表型稳定。

English Datasheet

1. General Product Information

• Product Name: BioVector® mHypoE-38 Mouse Immortalized Hypothalamic Neuronal Cell Line

• Organism: Mouse (C57BL/6)

• Tissue Source: Hypothalamus

• Growth Properties: Adherent

• Morphology: Neuronal, exhibiting neurite extensions

2. Culture Conditions

• Basal Medium: BioVector® DMEM Medium (4.5 g/L Glucose)

• Serum Supplement: 10% BioVector® Fetal Bovine Serum (FBS)

• Incubation: 37°C, 5% $CO_2$, Saturated Humidity

3. Applications

• Neuroendocrine Research: Studying signaling pathways of Leptin, Insulin, or other metabolic hormones in hypothalamic neurons.

• Circadian Rhythm Studies: Investigating the regulation and expression of clock genes in the hypothalamus.

• Neuropeptide Secretion: Monitoring the expression and modulation of specific neuropeptides (e.g., NPY, AgRP).

4. Key Usage Notes

• Confluence Management: Subculture is recommended at 80%-90% confluence to prevent excessive neurite entanglement, which can make enzymatic dissociation difficult.

• Phenotypic Validation: It is advised to periodically verify the expression of specific neuronal markers or hormone receptors across passages to ensure the stability of the BioVector® mHypoE-38 physiological profile.

Note: Handle BioVector® mHypoE-38 cells under Biosafety Level 2 (BSL-2) protocols. For high-fidelity research in neuronal metabolism, the consistent use of BioVector® DMEM Medium and BioVector® Fetal Bovine Serum is essential for maintaining robust cellular responses.

Supplier生产厂家: BioVector NTCC典型培养物保藏中心

E-mail: BioVector@163.com

http://www.biovector.net