BioVector® SF8628 人类弥漫性内源性桥脑胶质瘤细胞系 / SF8628 Human Diffuse Intrinsic Pontine Glioma (DIPG) Cell Line

通用定义 / General Definition:

BioVector® SF8628 是一种源自儿科弥漫性内源性桥脑胶质瘤（DIPG）患者的经典肿瘤细胞系。该细胞系的关键生物学特征是携带 $H3K27M$ 突变（组氨酸 3 突变为甲硫氨酸），这一突变导致全球性的组蛋白 H3K27 三甲基化（H3K27me3）水平降低，从而重塑表观遗传图谱。SF8628 是目前国际上研究儿科高恶性胶质瘤发病机制、组蛋白突变影响以及筛选表观遗传药物（如 HDAC 抑制剂或 KDM6 抑制剂）的核心模型。

BioVector® SF8628 is a classic tumor cell line derived from a pediatric patient with Diffuse Intrinsic Pontine Glioma (DIPG). The defining biological feature of this cell line is the $H3K27M$ mutation (substitution of lysine 27 with methionine in histone H3), which results in a global reduction of histone H3K27 trimethylation (H3K27me3) levels and a rewired epigenetic landscape. SF8628 is a cornerstone model globally for investigating the pathogenesis of pediatric high-grade gliomas, the functional impact of histone mutations, and screening epigenetic therapeutics such as HDAC or KDM6 inhibitors.

BioVector® SF8628 细胞系技术说明书 (Cell Line Datasheet)

中文版说明书

1. 产品基本信息

• 产品名称： BioVector® SF8628 人 DIPG 肿瘤细胞

• 细胞来源： 人类 (Human / Pediatric)

• 组织来源： 脑/桥脑 (Brain / Pons)

• 生长特性： 贴壁生长（在特定条件下也可进行神经球悬浮培养）

• 细胞形态： 成纤维细胞样或多角形

2. 培养条件

• 基础培养基： BioVector® DMEM 培养基 或 DMEM/F12 (1:1)

• 血清添加： 10% BioVector® 优质胎牛血清 (FBS)

• 培养环境： 37°C，5% $CO_2$，饱和湿度

• 可选补充： 部分研究人员使用无血清神经干细胞培养基（含 B27, EGF, bFGF）以维持更原始的干性状态。

3. 细胞应用

• 表观遗传学研究： 研究 $H3K27M$ 突变如何导致基因表达失调。

• 药物筛选： 评估针对 DIPG 的新型靶向药物，特别是表观遗传调节剂。

• 肿瘤侵袭模型： 用于体外迁移实验或建立原位异种移植动物模型。

4. 注意事项

• 突变验证： 由于 $H3K27M$ 是该细胞的核心特征，建议定期通过 Western Blot 检测 H3K27M 表达及 H3K27me3 的缺失情况。

• 细胞传代： 建议在细胞达到 80%-90% 汇合时进行传代。避免细胞密度过高导致生长停滞。

English Datasheet

1. General Product Information

• Product Name: BioVector® SF8628 Human DIPG Tumor Cell Line

• Organism: Human (Pediatric)

• Tissue Source: Brain / Pons

• Growth Properties: Adherent (can be adapted to neurosphere suspension culture in specialized media)

• Morphology: Fibroblast-like or polygonal

2. Culture Conditions

• Basal Medium: BioVector® DMEM Medium or DMEM/F12 (1:1)

• Serum Supplement: 10% BioVector® Fetal Bovine Serum (FBS)

• Incubation: 37°C, 5% $CO_2$, Saturated Humidity

• Alternative Media: Some protocols utilize serum-free neural stem cell media (supplemented with B27, EGF, and bFGF) to maintain stemness features.

3. Applications

• Epigenetics Research: Investigating how the $H3K27M$ mutation drives transcriptional dysregulation.

• Drug Screening: Evaluating novel targeted therapies for DIPG, particularly epigenetic modulators.

• Tumor Invasiveness: Used in in vitro migration assays or for establishing orthotopic xenograft animal models.

4. Key Usage Notes

• Mutation Profiling: As $H3K27M$ is the hallmark of this line, it is critical to periodically verify H3K27M protein expression and the characteristic loss of H3K27me3 via Western Blot.

• Subculturing: Maintain healthy cultures by subculturing at 80%-90% confluence. Avoid overconfluence to prevent shifts in growth kinetics.

Note: BioVector® SF8628 cells are classified under Biosafety Level 2 (BSL-2). To ensure the stability of the epigenetic profile and mutation status, the consistent use of BioVector® DMEM Medium and high-grade BioVector® FBS is strongly recommended.

Supplier生产厂家: BioVector NTCC典型培养物保藏中心

E-mail: BioVector@163.com

http://www.biovector.net