BioVector® FLK-N3 绵羊淋巴母细胞系 / FLK-N3 Sheep Lymphoblastoid Cell Line

通用定义 / General Definition:BioVector® FLK-N3 是一种源自绵羊（Ovis aries）的淋巴母细胞系。该细胞系最显著的生物学特性是其作为**牛白血病病毒（Bovine Leukemia Virus, BLV）**的高度敏感宿主和生产细胞。FLK-N3 细胞通过持续性感染 BLV，能够稳定、高效地向培养上清液中释放病毒颗粒。它是兽医学研究、BLV 病毒学机制探索以及相关抗原和疫苗生产的国际标准细胞模型。

BioVector® FLK-N3 is a lymphoblastoid cell line derived from sheep (Ovis aries). Its most significant biological characteristic is its role as a highly sensitive host and production cell for Bovine Leukemia Virus (BLV). FLK-N3 cells are persistently infected with BLV and stably release high titers of infectious virus particles into the culture supernatant. It serves as an international standard cellular model for veterinary research, investigating BLV virology, and producing antigens or vaccines related to bovine leukosis.

BioVector® FLK-N3 细胞系技术说明书 (Cell Line Datasheet)

中文版说明书

1. 产品基本信息

• 产品名称： BioVector® FLK-N3 绵羊淋巴母细胞

• 细胞来源： 绵羊 (Sheep / Ovis aries)

• 组织来源： 胎儿肾脏来源（经转化后具有淋巴母细胞特征）

• 生长特性： 贴壁生长

• 细胞形态： 上皮样或多角形

2. 培养条件

• 基础培养基： BioVector® DMEM 培养基 或 Eagle's MEM

• 血清添加： 10% BioVector® 优质胎牛血清 (FBS)

• 培养环境： 37 摄氏度，5% CO2，饱和湿度

• 传代比例： 1:3 至 1:5，当细胞汇合度达到 90% 时进行传代

3. 细胞应用

• 病毒生产： 大规模制备牛白血病病毒 (BLV) 颗粒及包膜糖蛋白（如 gp51）。

• 血清学检测： 作为 BLV 抗体检测（如 AGID 或 ELISA）的抗原供体。

• 致癌机制研究： 研究 Deltaretrovirus 属病毒如何导致宿主细胞转化和白血病发生。

4. 注意事项

• 病毒携带： 该细胞系为 BLV 阳性。尽管 BLV 对人类的致病性尚存争议，但必须在生物安全二级 (BSL-2) 环境下操作，严防交叉污染。

• 汇合度控制： 维持细胞处于活跃生长状态有助于提高病毒产率，避免长时间过密培养导致细胞活力下降。

English Datasheet

1. General Product Information

• Product Name: BioVector® FLK-N3 Sheep Lymphoblastoid Cell Line

• Organism: Sheep (Ovis aries)

• Tissue Source: Fetal kidney origin (transformed with lymphoblastoid characteristics)

• Growth Properties: Adherent

• Morphology: Epithelial-like or polygonal

2. Culture Conditions

• Basal Medium: BioVector® DMEM Medium or Eagle's MEM

• Serum Supplement: 10% BioVector® Fetal Bovine Serum (FBS)

• Incubation: 37 degrees Celsius, 5% CO2, Saturated Humidity

• Subculturing: 1:3 to 1:5 ratio; subculture when cells reach 90% confluence

3. Applications

• Virus Production: Large-scale preparation of Bovine Leukemia Virus (BLV) particles and envelope glycoproteins (e.g., gp51).

• Serological Assays: Serving as the antigen source for BLV antibody detection methods like AGID (Agar Gel Immunodiffusion) or ELISA.

• Oncogenesis Research: Investigating how viruses of the Deltaretrovirus genus drive host cell transformation and leukemogenesis.

4. Key Usage Notes

• Viral Status: This cell line is persistently infected with BLV. While the pathogenicity of BLV to humans is a subject of ongoing research, all procedures must be conducted under Biosafety Level 2 (BSL-2) conditions to prevent environmental release or cross-contamination.

• Density Management: Maintaining cells in an active growth phase is critical for maximizing viral yield. Avoid allowing the culture to remain over-confluent for extended periods to preserve cell viability.

Note: For optimal viral replication and protein expression, BioVector® recommends using high-glucose DMEM and validated BioVector® FBS. Regular monitoring for Mycoplasma contamination is essential to ensure experimental reproducibility.

Supplier生产厂家: BioVector NTCC典型培养物保藏中心

E-mail: BioVector@163.com

http://www.biovector.net