BioVector® HLA B46:01:01:01 人类白细胞抗原（HLA）B46:01 等位基因表达质粒 / HLA B46:01:01:01 Expression Plasmid

通用定义 / General Definition:BioVector® HLA B46:01:01:01 是一种携带人类白细胞抗原（HLA）B46:01 等位基因全长编码序列的表达质粒。HLA-B*46:01 是东亚人群（尤其是汉族）中非常普遍的 I 类 MHC 分子。它在免疫应答中起核心作用，负责向 CD8+ T 细胞递呈内源性抗原肽。该等位基因与多种生物医学现象显著相关，包括对 SARS-CoV 等病毒的易感性差异、重症药物超敏反应（如卡马西平诱导的 SJS/TEN）以及特定的自身免疫性疾病研究。

BioVector® HLA B46:01:01:01 is an expression plasmid containing the full-length coding sequence of the Human Leukocyte Antigen (HLA) B46:01 allele. HLA-B*46:01 is a highly prevalent Class I MHC molecule in East Asian populations, particularly Han Chinese. It plays a pivotal role in the immune response by presenting endogenous antigenic peptides to CD8+ T cells. This specific allele is significantly associated with various biomedical phenomena, including differential susceptibility to viruses like SARS-CoV, severe adverse drug reactions (e.g., Carbamazepine-induced SJS/TEN), and specific autoimmune disease research.

BioVector® HLA B*46:01:01:01 技术说明书 (Plasmid Datasheet)

中文版说明书

1. 产品基本信息

• 产品名称： BioVector® HLA B*46:01:01:01 表达质粒

• 基因简称： HLA-B

• 等位基因全称： HLA-B*46:01:01:01

• 启动子： CMV 或 EF-1 alpha（取决于具体版本）

• 抗性基因： * 大肠杆菌： 氨苄青霉素 (Ampicillin) 或 卡那霉素 (Kanamycin)

  • 真核筛选： 新霉素 (Neomycin/G418) 或 嘌呤霉素 (Puromycin)

• 标签/融合： 可选 C 端或 N 端携带 His/Flag 标签，或无标签原始序列。

2. 培养与扩增条件

• 宿主菌： BioVector® DH5-alpha 或 Stbl3。

• 培养基： BioVector® LB 培养基。

• 环境： 37 摄氏度，恒温振荡。

3. 载体应用

• 抗原递呈研究： 构建稳定表达 B*46:01 的细胞系，用于鉴定 T 细胞表位。

• 药物基因组学： 研究特定药物分子与 HLA-B*46:01 分子结合导致的免疫毒性。

• 疫苗研发： 评估针对东亚人群流行 HLA 亚型的疫苗免疫原性。

4. 注意事项

• 序列验证： HLA 基因具有高度多态性，建议使用 BioVector® 专用的 HLA 测序引物进行验证，确保 G-cluster 区段准确无误。

• 转染效率： 在某些原代淋巴细胞中转染效率较低，建议结合 BioVector® 慢病毒包装系统使用以提高稳定性。

English Datasheet

1. General Product Information

• Product Name: BioVector® HLA B*46:01:01:01 Expression Plasmid

• Gene Symbol: HLA-B

• Allele Full Name: HLA-B*46:01:01:01

• Promoter: CMV or EF-1 alpha (variant dependent)

• Selection Markers: * E. coli: Ampicillin or Kanamycin

  • Eukaryotic: Neomycin (G418) or Puromycin

• Tags/Fusion: Available with C-terminal or N-terminal His/Flag tags, or as a tag-free native sequence.

2. Culture and Amplification Conditions

• Host Strains: BioVector® DH5-alpha or Stbl3.

• Culture Medium: BioVector® LB Medium.

• Incubation: 37 degrees Celsius with constant shaking.

3. Applications

• Antigen Presentation Studies: Developing stable B*46:01-expressing cell lines for T-cell epitope mapping.

• Pharmacogenomics: Investigating immune-mediated toxicity caused by the binding of specific drug molecules to the HLA-B*46:01 protein.

• Vaccine Development: Evaluating the immunogenicity of vaccine candidates tailored to prevalent East Asian HLA subtypes.

4. Key Usage Notes

• Sequence Verification: Given the extreme polymorphism of HLA genes, it is critical to use BioVector® specialized HLA sequencing primers to verify the accuracy of the G-cluster and exons.

• Transduction Strategy: Transfection efficiency may be limited in primary lymphocytes; BioVector® recommends subcloning into a lentiviral backbone for stable and high-level expression.

Note: For research involving clinical drug sensitivity, BioVector® provides pre-validated cell lines (e.g., K562 or Jurkat) stably expressing HLA B*46:01:01:01. Always use BioVector® high-purity plasmid prep for functional immunology assays to minimize endotoxin interference.

Supplier生产厂家: BioVector NTCC典型培养物保藏中心

E-mail: BioVector@163.com

http://www.biovector.net