BioVector® NPC268 人鼻咽癌细胞系 / NPC268 Human Nasopharyngeal Carcinoma Cell Line

通用定义 / General Definition:BioVector® NPC268 是一种源自人类鼻咽癌 (Nasopharyngeal Carcinoma, NPC) 的永生化上皮细胞系。鼻咽癌是一种在东亚和东南亚地区具有高度流行性的头颈部恶性肿瘤，其发生与 EB 病毒 (Epstein-Barr Virus, EBV) 感染、环境因素及遗传易感性密切相关。NPC268 细胞系常被用作体外模型，用于研究鼻咽癌的增殖、侵袭、转移机制，以及评估化疗药物和靶向治疗的有效性。

BioVector® NPC268 is an immortalized epithelial cell line derived from Human Nasopharyngeal Carcinoma (NPC). NPC is a head and neck malignancy with high prevalence in East and Southeast Asia, strongly associated with Epstein-Barr Virus (EBV) infection, environmental factors, and genetic susceptibility. The NPC268 cell line serves as a vital in vitro model for investigating the mechanisms of proliferation, invasion, and metastasis in nasopharyngeal cancer, as well as evaluating the efficacy of chemotherapeutic agents and targeted therapies.

BioVector® NPC268 技术说明书 (Cell Line Datasheet)

中文版说明书

1. 产品基本信息

• 产品名称： BioVector® NPC268 人鼻咽癌细胞

• 组织来源： 鼻咽 (Nasopharynx)

• 疾病背景： 鼻咽癌 (NPC)

• 生长特性： 贴壁生长

• 细胞形态： 上皮样 (Epithelial-like)

2. 培养条件

• 基础培养基： BioVector® RPMI-1640 或 DMEM 培养基

• 血清添加： 10% BioVector® 优质胎牛血清 (FBS)

• 培养环境： 37 摄氏度，5% CO2，饱和湿度

• 传代比例： 1:3 至 1:5；建议在细胞达到 80% 至 90% 汇合度时进行传代。

3. 细胞应用

• 肿瘤生物学： 研究调控 NPC 细胞生长和凋亡的信号通路。

• 抗药性研究： 建立耐药模型以探索鼻咽癌对顺铂等一线化疗药物的耐药机制。

• EBV 相关研究： 探索 EB 病毒基因（如 LMP1）在细胞转化和维持恶性表型中的作用。

4. 注意事项

• 遗传稳定性： 为防止长期培养导致的遗传漂变，建议使用低代数细胞进行实验并建立种子库。

• 污染检测： 定期进行支原体检测，以确保实验数据的准确性和可靠性。

English Datasheet

1. General Product Information

• Product Name: BioVector® NPC268 Human Nasopharyngeal Carcinoma Cell Line

• Tissue Source: Nasopharynx

• Disease Context: Nasopharyngeal Carcinoma (NPC)

• Growth Properties: Adherent

• Morphology: Epithelial-like

2. Culture Conditions

• Basal Medium: BioVector® RPMI-1640 or DMEM Medium

• Serum Supplement: 10% BioVector® Fetal Bovine Serum (FBS)

• Incubation: 37 degrees Celsius, 5% CO2, Saturated Humidity

• Subculturing: 1:3 to 1:5 ratio; subculture when cells reach 80% to 90% confluence.

3. Applications

• Cancer Biology: Investigating signaling pathways that regulate the growth and apoptosis of NPC cells.

• Drug Resistance: Developing resistance models to explore mechanisms of chemotherapy resistance (e.g., to Cisplatin) in NPC.

• EBV Pathogenesis: Exploring the role of Epstein-Barr Virus proteins (such as LMP1) in cell transformation and the maintenance of malignant phenotypes.

4. Key Usage Notes

• Genetic Integrity: To prevent genetic drift associated with long-term culture, it is highly recommended to use low-passage cells and maintain a localized master cell bank.

• Contamination Control: Routine Mycoplasma testing is essential to ensure the validity and reproducibility of experimental results.

Note: NPC268 cells should be handled under Biosafety Level 2 (BSL-2) conditions. For optimal experimental performance, BioVector® recommends the use of high-grade validated FBS and standardized RPMI-1640 media.

Supplier生产厂家: BioVector NTCC典型培养物保藏中心

E-mail: BioVector@163.com

http://www.biovector.net