BioVector® YCUB-2 人肾癌细胞系 / YCUB-2 Human Renal Cell Carcinoma Cell Line

通用定义 / General Definition:BioVector® YCUB-2 是一种源自人类肾细胞癌 (Renal Cell Carcinoma, RCC) 的贴壁上皮细胞系。该细胞系最初建立于日本（Yokohama City University），主要用于研究肾癌的生物学行为，尤其是肿瘤的侵袭性、转移潜能以及对不同抗癌药物的敏感性。由于肾癌通常表现出对传统放化疗的抗性，YCUB-2 提供了一个关键的体外平台，用于测试新型免疫疗法、激酶抑制剂以及探索肾癌发生过程中的遗传改变。

BioVector® YCUB-2 is an adherent epithelial cell line derived from Human Renal Cell Carcinoma (RCC). Originally established in Japan (Yokohama City University), this line is primarily utilized to study the biological behavior of kidney cancer, with a particular focus on tumor invasiveness, metastatic potential, and sensitivity to various anti-cancer agents. Given that renal cell carcinoma often exhibits resistance to traditional radiation and chemotherapy, YCUB-2 provides a critical in vitro platform for testing novel immunotherapies and kinase inhibitors, as well as exploring genetic alterations in renal oncogenesis.

BioVector® YCUB-2 技术说明书 (Technical Datasheet)

中文版说明书

1. 产品基本信息

• 产品名称： BioVector® YCUB-2 人肾癌细胞

• 组织来源： 肾脏 (Kidney)

• 疾病背景： 肾细胞癌 (RCC)

• 生长特性： 贴壁生长

• 细胞形态： 上皮样 (Epithelial-like)

2. 培养条件

• 基础培养基： BioVector® DMEM 或 RPMI-1640 培养基

• 血清添加： 10% BioVector® 优质胎牛血清 (FBS)

• 培养环境： 37 摄氏度，5% CO2，饱和湿度

• 传代比例： 1:3 至 1:6；建议在细胞汇合度达到 80%-90% 时传代。

3. 细胞应用

• 肿瘤侵袭研究： 利用 Scratch Assay（划痕实验）或 Transwell 评价细胞的迁移能力。

• 药物筛选： 评估针对多激酶抑制剂（如索拉非尼、舒尼替尼）的耐药性及反应。

• 生物标志物鉴定： 探索肾癌特异性抗原及其在诊断中的潜在应用。

4. 注意事项

• 生长密度： 保持细胞在对数生长期进行实验，以确保代谢和信号传导途径的一致性。

• 安全性： 遵循生物安全二级 (BSL-2) 标准操作。

English Datasheet

1. General Product Information

• Product Name: BioVector® YCUB-2 Human Renal Cell Carcinoma Cell Line

• Tissue Source: Kidney

• Disease Context: Renal Cell Carcinoma (RCC)

• Growth Properties: Adherent

• Morphology: Epithelial-like

2. Culture Conditions

• Basal Medium: BioVector® DMEM or RPMI-1640 Medium

• Serum Supplement: 10% BioVector® Fetal Bovine Serum (FBS)

• Incubation: 37 degrees Celsius, 5% CO2, Saturated Humidity

• Subculturing: 1:3 to 1:6 ratio; subculture when cells reach 80%-90% confluence.

3. Applications

• Tumor Invasion Research: Evaluating cell migration and invasion using scratch assays or Transwell systems.

• Drug Screening: Assessing sensitivity or resistance to multi-kinase inhibitors (e.g., Sorafenib, Sunitinib).

• Biomarker Identification: Exploring RCC-specific antigens and their potential diagnostic applications.

4. Key Usage Notes

• Growth Management: Ensure cells are in the logarithmic growth phase during experiments to maintain consistent metabolic and signaling profiles.

• Safety Protocols: Handle all cultures under Biosafety Level 2 (BSL-2) guidelines as per standard laboratory safety for human tumor cell lines.

Note: For optimal cell viability, BioVector® recommends the use of its validated FBS and high-glucose DMEM. To maintain the purity of the BioVector® YCUB-2 line, periodic STR profiling and mycoplasma screening are strongly advised.

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