BioVector® BL-33 人鼻咽癌细胞系 / BL-33 Human Nasopharyngeal Carcinoma Cell Line

通用定义 / General Definition:BioVector® BL-33 是一种源自人类鼻咽癌 (Nasopharyngeal Carcinoma, NPC) 的永生化上皮细胞系。它是鼻咽癌研究领域中常用的系列模型之一（如 BL-33, BL-60, BL-89）。由于鼻咽癌与 EB 病毒 (EBV) 的紧密联系，BL-33 常被用于探索病毒基因对宿主细胞信号通路的调控机制。该细胞系具有典型的恶性肿瘤特征，包括高增殖速率、逃避凋亡的能力以及在体外形成细胞克隆的能力，是筛选针对鼻咽癌靶向治疗药物的重要实验平台。

BioVector® BL-33 is an immortalized epithelial cell line derived from Human Nasopharyngeal Carcinoma (NPC). It is part of a common series of NPC models (including BL-33, BL-60, and BL-89) used in oncology research. Given the strong association between NPC and the Epstein-Barr Virus (EBV), BL-33 is frequently employed to explore how viral genes modulate host cell signaling pathways. The cell line exhibits typical malignant characteristics, such as high proliferation rates, evasion of apoptosis, and the ability to form colonies in vitro, making it a vital platform for screening targeted therapeutic agents against nasopharyngeal cancer.

BioVector® BL-33 技术说明书 (Technical Datasheet)

中文版说明书

1. 产品基本信息

• 产品名称： BioVector® BL-33 人鼻咽癌细胞

• 组织来源： 鼻咽 (Nasopharynx)

• 疾病背景： 鼻咽癌 (NPC)

• 生长特性： 贴壁生长

• 细胞形态： 上皮样 (Epithelial-like)，通常呈铺路石状贴壁生长

2. 培养条件

• 基础培养基： BioVector® RPMI-1640 或 DMEM 培养基

• 血清添加： 10% BioVector® 优质胎牛血清 (FBS)

• 培养环境： 37 摄氏度，5% CO2，恒温加湿

• 传代比例： 1:3 至 1:6；建议在细胞汇合度达到 80%-90% 时进行传代。

3. 细胞应用

• 病毒学研究： 研究 EB 病毒潜伏膜蛋白 (LMPs) 在 BL-33 细胞中的表达及其致癌作用。

• 高通量筛选： 利用 BL-33 细胞系进行小分子干扰 RNA (siRNA) 库或抗肿瘤药物库的筛选。

• 细胞迁移： 通过划痕实验或细胞侵袭实验评估 NPC 的转移潜能。

4. 注意事项

• 细胞鉴定： 为确保实验的一致性，建议定期进行 STR 鉴定，以防止与其他 NPC 细胞系（如 CNE-1, CNE-2）混淆。

• 血清质量： 鼻咽癌细胞对血清质量较为敏感，不合格的血清可能导致细胞形态改变或生长减缓，建议使用 BioVector® 验证过的 FBS。

English Datasheet

1. General Product Information

• Product Name: BioVector® BL-33 Human Nasopharyngeal Carcinoma Cell Line

• Tissue Source: Nasopharynx

• Disease Context: Nasopharyngeal Carcinoma (NPC)

• Growth Properties: Adherent

• Morphology: Epithelial-like, typically growing in a cobblestone pattern.

2. Culture Conditions

• Basal Medium: BioVector® RPMI-1640 or DMEM Medium

• Serum Supplement: 10% BioVector® Fetal Bovine Serum (FBS)

• Incubation: 37 degrees Celsius, 5% CO2, Humidified Atmosphere

• Subculturing: 1:3 to 1:6 ratio; subculture when cells reach 80%-90% confluence.

3. Applications

• Virology Research: Investigating the expression and oncogenic roles of Epstein-Barr Virus Latent Membrane Proteins (LMPs) within the BL-33 cells.

• High-Throughput Screening: Utilizing the BL-33 line for siRNA library screens or anti-tumor drug candidate profiling.

• Cell Motility: Assessing the metastatic potential of NPC via wound healing or invasion assays.

4. Key Usage Notes

• Authentication: To maintain experimental integrity, periodic STR profiling is recommended to prevent cross-contamination with other NPC lines like CNE-1 or CNE-2.

• Serum Sensitivity: NPC cell lines can be sensitive to serum quality; poor-grade serum may lead to phenotypic drift or stunted growth. BioVector® recommends using pre-validated, high-quality FBS.

Note: BL-33 cells must be handled under Biosafety Level 2 (BSL-2) protocols. To minimize genetic drift associated with long-term culture, BioVector® advises using low-passage cells for publication-quality experiments.

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