BioVector® BL-29 人鼻咽癌细胞系 / BL-29 Human Nasopharyngeal Carcinoma Cell Line

通用定义 / General Definition:BioVector® BL-29 是一种源自人类鼻咽癌 (Nasopharyngeal Carcinoma, NPC) 的永生化上皮细胞系。它是研究鼻咽癌生物学特性的经典体外模型之一。BL-29 细胞常被用于探索 NPC 细胞的代谢重编程、细胞信号传导失调以及对放射治疗和化学治疗药物的反应机制。与 BL-31 和 BL-33 类似，该细胞系在模拟东亚高发的鼻咽恶性肿瘤方面具有重要的科研价值。

BioVector® BL-29 is an immortalized epithelial cell line derived from Human Nasopharyngeal Carcinoma (NPC). It is one of the classic in vitro models used to study the biological characteristics of nasopharyngeal cancer. BL-29 cells are frequently utilized to explore metabolic reprogramming, dysregulated cellular signaling, and the mechanisms of response to radiotherapy and chemotherapeutic agents. Similar to BL-31 and BL-33, this cell line holds significant research value in modeling nasopharyngeal malignancies, which have a high incidence in East Asia.

BioVector® BL-29 技术说明书 (Technical Datasheet)

中文版说明书

1. 产品基本信息

• 产品名称： BioVector® BL-29 人鼻咽癌细胞

• 组织来源： 鼻咽 (Nasopharynx)

• 疾病背景： 鼻咽癌 (NPC)

• 生长特性： 贴壁生长

• 细胞形态： 上皮样 (Epithelial-like)，呈多角形，排列紧密

2. 培养条件

• 基础培养基： BioVector® RPMI-1640 或 DMEM 培养基

• 血清添加： 10% BioVector® 优质胎牛血清 (FBS)

• 培养环境： 37 摄氏度，5% CO2，饱和湿度

• 传代比例： 1:3 至 1:4；建议在细胞汇合度达到 85% 左右时进行传代。

3. 细胞应用

• 放疗敏感性评估： 研究 BL-29 细胞在电离辐射后的 DNA 损伤修复能力。

• 信号通路分析： 探索调控 NPC 生长和存活的激酶通路（如 PI3K/Akt, MAPK 等）。

• 非编码 RNA 研究： 鉴定影响鼻咽癌进展的特异性 miRNA 或 lncRNA。

4. 注意事项

• 细胞系验证： 由于 NPC 细胞系数量较多，建议使用 BioVector® 提供的 STR 鉴定数据进行对比，防止交叉污染。

• 低氧实验： NPC 肿瘤常伴有缺氧微环境，BL-29 细胞在低氧诱导下表现出显著的表型改变。

English Datasheet

1. General Product Information

• Product Name: BioVector® BL-29 Human Nasopharyngeal Carcinoma Cell Line

• Tissue Source: Nasopharynx

• Disease Context: Nasopharyngeal Carcinoma (NPC)

• Growth Properties: Adherent

• Morphology: Epithelial-like, polygonal, growing in dense clusters.

2. Culture Conditions

• Basal Medium: BioVector® RPMI-1640 or DMEM Medium

• Serum Supplement: 10% BioVector® Fetal Bovine Serum (FBS)

• Incubation: 37 degrees Celsius, 5% CO2, Saturated Humidity

• Subculturing: 1:3 to 1:4 ratio; subculture when cells reach approximately 85% confluence.

3. Applications

• Radiosensitivity Assessment: Investigating the DNA damage repair capacity of BL-29 cells following ionizing radiation.

• Signaling Pathway Analysis: Exploring kinase pathways (e.g., PI3K/Akt, MAPK) that regulate growth and survival in NPC.

• Non-coding RNA Research: Identifying specific miRNAs or lncRNAs that influence the progression of nasopharyngeal cancer.

4. Key Usage Notes

• Cell Authentication: Due to the large number of existing NPC cell lines, BioVector® strongly recommends comparing results with STR profiling data to prevent cross-contamination.

• Hypoxia Studies: Since NPC tumors often harbor a hypoxic microenvironment, BL-29 cells exhibit significant phenotypic changes when cultured under low-oxygen conditions.

Note: BL-29 cells should be handled under Biosafety Level 2 (BSL-2) conditions. To ensure experimental reproducibility, BioVector® recommends using standardized media and high-quality FBS, and suggests maintaining a low passage number during functional assays.

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