BioVector® Butyricimonas virosa 丁酸单胞菌属菌株 / BioVector® Butyricimonas virosa Strain

通用定义 / General Definition:

BioVector® Butyricimonas virosa 是一种属于拟杆菌目 (Bacteroidales)、臭气杆菌科 (Odoribacteraceae) 的革兰氏阴性、专性厌氧、不产芽孢杆菌。该菌株最初从人类粪便样本中分离得到。其最显著的代谢特征是能够产生大量的丁酸 (Butyrate)，这是一种对肠道健康至关重要的短链脂肪酸 (SCFA)。B. virosa 被认为是肠道微生态系统中的重要成员，在维持免疫稳态、抗炎作用以及调节宿主代谢（如葡萄糖稳态）中发挥着潜在的关键作用。

BioVector® Butyricimonas virosa is a Gram-negative, obligately anaerobic, non-spore-forming rod-shaped bacterium belonging to the order Bacteroidales and the family Odoribacteraceae. Originally isolated from human faecal samples, its most significant metabolic hallmark is the production of substantial amounts of butyrate, a short-chain fatty acid (SCFA) vital for intestinal health. B. virosa is recognized as an important commensal in the gut microbiome, playing potential roles in maintaining immune homeostasis, exerting anti-inflammatory effects, and regulating host metabolism (e.g., glucose homeostasis).

BioVector® B. virosa 技术说明书 (Technical Datasheet)

中文版说明书 (Chinese Datasheet)

1. 产品基本信息

• 产品名称： BioVector® Butyricimonas virosa 菌株

• 生物分类： 丁酸单胞菌属 (Butyricimonas)

• 基因组特征： 含有参与碳水化合物发酵产生丁酸的完整酶系（如丁酸激酶通路）。

• 形态特征： 短杆状，通常长 0.5–1.0 $\mu$m。

2. 培养条件

• 培养基： BioVector® 加强梭菌培养基 (RCM) 或 脑心浸液培养基 (BHI)，建议添加 5% 羊血或血红素/维生素 K1。

• 培养温度： 37 摄氏度。

• 厌氧环境： 必须在严格厌氧条件下培养（$N_2$ 80%, $CO_2$ 10%, $H_2$ 10%）。

• 生长特性： 在琼脂平板上形成圆形、半透明至白色的微小菌落。

3. 菌株应用

• 代谢组学研究： 作为丁酸产生菌模型，研究短链脂肪酸对肠道上皮屏障功能的增强作用。

• 疾病关联研究： 探讨该菌在肥胖、2型糖尿病及炎症性肠病 (IBD) 患者肠道中的丰度变化及其生物学意义。

• 益生元筛选： 评估不同膳食纤维（如菊粉、抗性淀粉）对 B. virosa 生长及产酸效率的促进作用。

4. 注意事项

• 氧敏感性： 该菌对氧气极其敏感，接种及操作过程需在厌氧工作站内完成，暴露于空气中会导致活力迅速丧失。

• 生物安全： 通常归类为生物安全一级 (BSL-1)，但处理来自临床样本的变体时应遵循 BSL-2 规范。

English Datasheet

1. General Product Information

• Product Name: BioVector® Butyricimonas virosa Strain

• Biological Classification: Genus Butyricimonas

• Genomic Features: Possesses a complete set of enzymes for carbohydrate fermentation leading to butyrate (e.g., butyrate kinase pathway).

• Morphology: Short rods, typically 0.5–1.0 $\mu$m in length.

2. Culture Conditions

• Media: BioVector® Reinforced Clostridial Medium (RCM) or Brain Heart Infusion (BHI) broth/agar, supplemented with 5% sheep blood or hemin/vitamin K1.

• Incubation Temperature: 37°C.

• Anaerobic Environment: Must be cultured under strictly anaerobic conditions ($N_2$ 80%, $CO_2$ 10%, $H_2$ 10%).

• Growth Characteristics: Forms small, circular, translucent to white colonies on anaerobic blood agar.

3. Applications

• Metabolomics: Serving as a model for butyrate-producing bacteria to study the enhancement of intestinal barrier integrity by SCFAs.

• Microbiome Association Studies: Investigating the correlation between B. virosa abundance and metabolic disorders such as obesity, Type 2 Diabetes, and Inflammatory Bowel Disease (IBD).

• Prebiotic Screening: Evaluating the fermentation efficiency of various dietary fibers (e.g., inulin, resistant starch) by this strain.

4. Key Usage Notes

• Oxygen Sensitivity: This strain is highly sensitive to oxygen. All manipulations, including inoculation and subculturing, should be performed inside an anaerobic chamber to prevent loss of viability.

• Storage: Long-term storage requires cryopreservation in anaerobic glycerol stock at -80°C or lyophilization.

注意 / Note:Butyricimonas virosa 在肠道中的减少往往与代谢炎症相关。BioVector® 建议在共培养实验中使用专门设计的厌氧培养基，以模拟肠道内复杂的营养竞争环境。

The depletion of Butyricimonas virosa in the gut is often associated with metabolic inflammation. BioVector® recommends using specialized anaerobic media in co-culture experiments to simulate the complex nutritional competition within the intestinal environment.

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