BioVector® NCTC 8241 (Acinetobacter calcoaceticus) 不动杆菌菌株 / BioVector® NCTC 8241 (Acinetobacter calcoaceticus) Strain

通用定义 / General Definition:BioVector® NCTC 8241 是不动杆菌属 (Acinetobacter) 的模式菌株，分类学上属于 不动杆菌 (Acinetobacter calcoaceticus)。不动杆菌属是一类革兰氏阴性、不发酵糖类、非动力性的球杆菌，广泛分布于土壤和水体中。NCTC 8241 菌株作为该属的典型代表，被广泛用于微生物分类学鉴定、抗生素耐药性机制研究以及生物修复（如降解碳氢化合物）等领域。它是区分不动杆菌属内不同物种（特别是与临床相关的鲍曼不动杆菌复合物）的重要参考标准。

BioVector® NCTC 8241 is a type strain of the genus Acinetobacter, taxonomically identified as Acinetobacter calcoaceticus. Members of the Acinetobacter genus are Gram-negative, non-fermentative, non-motile coccobacilli that are ubiquitous in soil and water environments. As a representative strain, NCTC 8241 is extensively used in microbial taxonomy, studies of antibiotic resistance mechanisms, and bioremediation (e.g., degradation of hydrocarbons). It serves as a fundamental reference standard for distinguishing species within the genus, particularly in differentiating it from the clinically significant A. baumannii complex.

BioVector® NCTC 8241 技术说明书 (Technical Datasheet)

中文版说明书 (Chinese Datasheet)

1. 产品基本信息

• 产品名称： BioVector® NCTC 8241 (Acinetobacter calcoaceticus) 菌株

• 其他编号： ATCC 23055, DSM 30006

• 生物分类： 不动杆菌属 (Acinetobacter)

• 形态特征： 革兰氏阴性球杆菌，成对或链状排列；在对数生长期呈杆状，在稳定期趋于球状。

2. 培养条件

• 培养基： BioVector® 营养肉汤 (Nutrient Broth) 或 胰蛋白酶大豆琼脂 (TSA)。

• 培养温度： 最适生长温度为 30 摄氏度（注意：许多 A. calcoaceticus 菌株在 37 摄氏度下生长较弱，这与鲍曼不动杆菌不同）。

• 培养环境： 专性需氧，建议摇床转速为 200 rpm。

• 生长特性： 菌落通常呈圆形、光滑、凸起，边缘整齐。

3. 菌株应用

• 分类与鉴定： 作为不动杆菌属的指纹图谱和基因组比对的标准参考菌株。

• 生物修复： 研究其降解原油、酚类化合物及其他有机污染物的代谢途径。

• 抗药性研究： 比较环境菌株与临床菌株在耐药基因获取和外排泵机制上的差异。

4. 注意事项

• 温度敏感性： 严格遵守 30°C 的培养建议，高温可能导致生长受限。

• 安全性： 归类为生物安全一级 (BSL-1)。虽然通常被认为对健康人类无致病性，但在处理时仍需遵循标准实验室无菌操作。

English Datasheet

1. General Product Information

• Product Name: BioVector® NCTC 8241 (Acinetobacter calcoaceticus) Strain

• Alternative Designations: ATCC 23055, DSM 30006

• Biological Classification: Genus Acinetobacter

• Morphology: Gram-negative coccobacilli, appearing in pairs or chains; typically rod-shaped during log phase and more coccoid during stationary phase.

2. Culture Conditions

• Media: BioVector® Nutrient Broth or Tryptic Soy Agar (TSA).

• Incubation Temperature: Optimal growth at 30°C. (Note: Unlike A. baumannii, many A. calcoaceticus strains grow poorly or not at all at 37°C).

• Environment: Obligately aerobic; recommended shaking at 200 rpm for liquid cultures.

• Growth Characteristics: Colonies are generally circular, smooth, convex, and entire.

3. Applications

• Taxonomy and Diagnostics: Serving as the definitive reference strain for genomic comparisons and biochemical profiling within the Acinetobacter genus.

• Environmental Bioremediation: Investigating metabolic pathways for the degradation of crude oil, phenols, and other complex hydrocarbons.

• Comparative Genomics: Studying the evolution of antibiotic resistance by comparing environmental type strains with clinical isolates.

4. Key Usage Notes

• Thermal Constraints: Adherence to the 30°C incubation temperature is critical for consistent growth and phenotypic expression.

• Safety Protocols: Generally categorized as Biosafety Level 1 (BSL-1). While typically non-pathogenic to healthy individuals, standard aseptic laboratory techniques should be maintained.

注意 / Note: NCTC 8241 的天然遗传转化能力是其重要特征。BioVector® 建议在进行基因重组实验时，利用其高效率的自然转化特性来构建突变体。A key feature of NCTC 8241 is its natural genetic competence. BioVector® recommends leveraging its high efficiency for natural transformation when constructing mutants or performing gene recombination experiments.

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