BioVector® Thy0517 人胸腺瘤细胞系 / BioVector® Thy0517 Human Thymoma Cell Line

通用定义 / General Definition:

BioVector® Thy0517 是一种源自人胸腺瘤 (Thymoma) 组织的特异性细胞系。胸腺瘤是源于胸腺上皮细胞的肿瘤，临床上常与重症肌无力（Myasthenia Gravis）等自身免疫性疾病密切相关。Thy0517 细胞系保留了胸腺上皮细胞的关键生化特征，包括特定细胞角蛋白的表达以及参与 T 细胞选择的微环境模拟能力。它是研究胸腺肿瘤生物学、胸腺上皮与 T 细胞相互作用以及副肿瘤综合征发病机制的重要体外模型。

BioVector® Thy0517 is a specialized cell line derived from human Thymoma tissue. Thymomas are tumors originating from the thymic epithelial cells and are frequently associated with paraneoplastic autoimmune conditions, most notably Myasthenia Gravis (MG). The Thy0517 cell line retains essential biochemical characteristics of thymic epithelia, including the expression of specific cytokeratins and the ability to simulate the microenvironment involved in T-cell selection. It serves as a vital in vitro model for studying thymic oncology, epithelial-T cell interactions, and the pathogenesis of thymus-related autoimmune syndromes.

BioVector® Thy0517 技术说明书 (Technical Datasheet)

中文版说明书 (Chinese Datasheet)

1. 产品基本信息

• 产品名称： BioVector® Thy0517 人胸腺瘤细胞

• 组织来源： 胸腺 (Thymus)

• 疾病背景： 胸腺瘤 (Thymoma)

• 生长特性： 贴壁生长

• 细胞形态： 上皮样，通常呈多角形，具有典型的上皮细胞超微结构。

2. 培养条件

• 基础培养基： BioVector® RPMI-1640 或 DMEM/F12 混合培养基。

• 血清添加： 10% - 15% BioVector® 优质胎牛血清 (FBS)。

• 关键添加剂： 某些实验方案建议添加上皮细胞生长因子 (EGF) 和氢化可的松以促进生长。

• 培养环境： 37 摄氏度，5% $CO_2$。

• 传代比例： 1:2 至 1:4；建议在细胞汇合度达到 80-90% 时传代。

3. 细胞应用

• 肿瘤生物学： 研究胸腺瘤细胞的增殖信号通路（如 EGFR、IGF-1R 通路）。

• 免疫学模拟： 与原代 T 细胞共培养，研究胸腺上皮肿瘤对 T 细胞发育和自身耐受的影响。

• 药物筛选： 测试针对难治性或转移性胸腺癌的新型化疗药物和靶向疗法。

4. 注意事项

• 异质性： 胸腺瘤细胞在体外培养中可能表现出一定的形态异质性，需定期观察。

• 验证： 建议通过检测细胞角蛋白（Cytokeratins）或抗原呈递分子（MHC II）来确认其上皮属性。

English Datasheet

1. General Product Information

• Product Name: BioVector® Thy0517 Human Thymoma Cell Line

• Tissue Source: Thymus

• Disease Context: Thymoma

• Growth Properties: Adherent

• Morphology: Epithelial-like; typically polygonal with characteristic epithelial ultrastructures.

2. Culture Conditions

• Basal Medium: BioVector® RPMI-1640 or DMEM/F12 (1:1) mixture.

• Serum Supplement: 10% - 15% BioVector® Fetal Bovine Serum (FBS).

• Supplements: Recombinant human EGF and Hydrocortisone may be added to optimize the growth of the epithelial lineage.

• Incubation: 37 degrees Celsius, 5% $CO_2$.

• Subculturing: 1:2 to 1:4 ratio; subculture when the monolayer reaches 80-90% confluence.

3. Applications

• Cancer Research: Investigating oncogenic signaling pathways (e.g., EGFR, IGF-1R) specific to thymic epithelial tumors.

• Immunological Modeling: Co-culture studies with T-lymphocytes to examine how thymomas disrupt central tolerance and T-cell education.

• Drug Discovery: Screening therapeutic compounds for the treatment of advanced thymic malignancies and associated autoimmune disorders.

4. Key Usage Notes

• Phenotypic Stability: Epithelial markers may fluctuate with high passage numbers; BioVector® recommends verifying the expression of cytokeratins to ensure cell identity.

• Adhesion Profile: Thy0517 cells require a stable surface for attachment; ensure the use of high-quality tissue culture-treated flasks.

注意 / Note: BioVector® Thy0517 应在生物安全二级 (BSL-2) 条件下操作。由于其来源于人体肿瘤组织，所有实验废弃物必须严格按照生物危险品处理规范进行处置。

BioVector® Thy0517 should be handled under Biosafety Level 2 (BSL-2) conditions. Given its human tumor origin, all experimental waste must be disposed of in strict accordance with biohazardous material protocols.

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

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