BioVector® Muribaculum intestinale 菌株 / BioVector® Muribaculum intestinale Strain

通用定义 / General Definition:BioVector® Muribaculum intestinale 是一种属于拟杆菌目 (Bacteroidales)、Muribaculaceae 科（原称 S24-7 家族）的革兰氏阴性、专性厌氧、不产芽孢杆菌。该菌株是小鼠肠道微生物群中最占优势的成员之一，但在人类肠道中分布较少。M. intestinale 以其极强的复杂碳水化合物（如植物源性多糖）降解能力而闻名。作为 Muribaculaceae 科的模式物种，它是研究啮齿动物肠道微生态稳态、宿主-微生物共进化以及膳食纤维代谢对粘膜免疫影响的关键生物模型。

BioVector® Muribaculum intestinale is a Gram-negative, obligately anaerobic, non-spore-forming rod-shaped bacterium belonging to the order Bacteroidales and the family Muribaculaceae (formerly known as the S24-7 family). This strain is one of the most dominant members of the mouse gut microbiota, though it is less prevalent in humans.M. intestinale is renowned for its specialized capacity to degrade complex carbohydrates, particularly plant-derived polysaccharides. As the type species of its family, it serves as a pivotal biological model for studying rodent gut homeostasis, host-microbe co-evolution, and the impact of dietary fiber metabolism on mucosal immunity.

BioVector® M. intestinale 技术说明书 (Technical Datasheet)

中文版说明书 (Chinese Datasheet)

1. 产品基本信息

• 产品名称： BioVector® Muribaculum intestinale 菌株

• 其他编号： YL27, DSM 28989

• 基因组特征： 基因组中含有大量编码碳水化合物活性酶（CAZymes）的基因，专门用于降解复杂的植物多糖和宿主粘蛋白。

• 主要代谢产物： 产生乙酸 (Acetate)、琥珀酸 (Succinate) 和丙酸 (Propionate)。

2. 培养条件

• 基础培养基： BioVector® 改良 PYG 培养基（含葡萄糖、酵母膏、蛋白胨）或 BHI 培养基，通常需添加血红素和维生素 K1。

• 培养温度： 37 摄氏度。

• 厌氧要求： 必须在严格厌氧环境下培养。

• 生长特性： 生长相对缓慢，在固体培养基上形成微小、半透明的菌落。

3. 菌株应用

• 营养代谢研究： 研究不同种类的膳食纤维如何选择性地富集 Muribaculaceae 科细菌。

• 肠道定殖实验： 在无菌 (Germ-free) 小鼠模型中重建肠道菌群，研究其对屏障功能的影响。

• 比较基因组学： 探讨小鼠优势菌群与人类优势菌群（如拟杆菌属）在功能位点上的异同。

4. 注意事项

• 培养挑战： 该菌对培养条件较为苛刻，建议在培养基中添加少量的原代肠道内容物提取物或特定的多糖底物以促进初始生长。

• 保存建议： 建议使用含有 15-20% 甘油的厌氧冻存管于 -80°C 保存。

English Datasheet

1. General Product Information

• Product Name: BioVector® Muribaculum intestinale Strain

• Alternative Designations: YL27, DSM 28989

• Genomic Features: The genome is highly enriched with genes encoding Carbohydrate-Active Enzymes (CAZymes), specialized for the breakdown of complex plant polysaccharides and host-derived mucins.

• Metabolic Output: Produces acetate, succinate, and propionate as major fermentation end-products.

2. Culture Conditions

• Basal Medium: BioVector® Modified PYG (Peptone Yeast Glucose) or BHI broth, supplemented with hemin and vitamin K1.

• Incubation Temperature: 37°C.

• Anaerobic Requirement: Must be maintained under strictly anaerobic conditions.

• Growth Characteristics: Exhibits slow growth kinetics; forms minute, translucent colonies on anaerobic agar plates.

3. Applications

• Nutritional Metabolism: Investigating how specific dietary fibers selectively promote the expansion of Muribaculaceae populations.

• Gnotobiotic Research: Colonizing germ-free mouse models to study the impact of dominant commensals on mucosal immune maturation.

• Evolutionary Biology: Studying host-specific microbial adaptations and the niche partitioning between Muribaculaceae in rodents and Bacteroides in humans.

4. Key Usage Notes

• Cultivation Difficulty:M. intestinale can be fastidious; BioVector® suggests supplementing media with specific complex glycans to improve recovery from cryopreservation.

• Sample Integrity: Due to its dominance in mice, ensure that experimental cross-contamination from environmental mouse sources is strictly controlled when using this strain in defined consortia.

注意 / Note:Muribaculum intestinale 是了解小鼠模型中“健康”肠道状态的基础。BioVector® 提醒研究者，该菌在抗生素处理后极易受到抑制，但在高纤维饮食下会迅速恢复。Muribaculum intestinale is fundamental to understanding the "healthy" gut state in mouse models. BioVector® notes that while this strain is highly susceptible to antibiotic suppression, it recovers rapidly under high-fiber dietary regimens.

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