BioVector® 解脲支原体 ATCC 33175 菌株 / BioVector® Ureaplasma urealyticum ATCC 33175 Strain

重要澄清 / Important Clarification: > 之前的 ATCC 33175 通常指代口腔病原菌 Aggregatibacter actinomycetemcomitans。如果您所指的是 解脲支原体 (Ureaplasma urealyticum)，其标准的模式菌株通常为 ATCC 27618 (T960)。然而，针对解脲支原体系列科研需求，我们为您提供如下针对 Ureaplasma urealyticum 的通用技术说明。

通用定义 / General Definition:

BioVector® 解脲支原体是一种极小的、缺乏细胞壁的柔膜菌纲 (Mollicutes) 微生物。它是人类泌尿生殖道中常见的共生菌，但在特定条件下可引发非淋菌性尿道炎、不孕不育、绒毛膜羊膜炎及新生儿呼吸道疾病。其代谢上最显著的特征是具有高度活跃的尿素酶 (Urease)，通过水解尿素产生氨来获得能量（产生质子梯度）。由于缺乏细胞壁，它对内酰胺类抗生素天然耐药。

BioVector® Ureaplasma urealyticum is an extremely small, cell-wall-deficient microorganism belonging to the class Mollicutes. It is a common commensal of the human urogenital tract but can become pathogenic, causing non-gonococcal urethritis, infertility, chorioamnionitis, and neonatal respiratory infections. Its most defining metabolic trait is the presence of highly active urease, which hydrolyzes urea to generate ammonia and create a proton motive force for energy. Being cell-wall-less, it is naturally resistant to beta-lactam antibiotics.

BioVector® 解脲支原体技术说明书 (Technical Datasheet)

中文版说明书 (Chinese Datasheet)

1. 产品基本信息

• 产品名称： BioVector® 解脲支原体 (Ureaplasma urealyticum)

• 基因组特征： 基因组极小（约 0.75-1.0 Mbp），高度依赖宿主或培养基提供营养。

• 形态特征： 多形性（球形、梨形），直径仅 0.1-1.0 $\mu$m；革兰氏染色不显色，需用 Giemsa 染色。

2. 培养条件

• 基础培养基： BioVector® Ureaplasma 培养基 (10号培养基)，需含有牛血清、尿素和生长因子。

• pH 要求： 初始 pH 需精确调至 6.0 ($\pm 0.2$)（该菌在酸性环境下生长较好）。

• 大气环境： 需在 5% $CO_2$ 环境下培养。

• 代谢指示： 培养基中含酚红，当尿素被水解产生氨导致 pH 升高时，培养基颜色会由黄变红。

3. 菌株应用

• 抗生素耐药性监测： 研究大环内酯类、四环素类及喹诺酮类药物对生殖道支原体的疗效。

• 生殖健康研究： 探讨解脲支原体对精子活力、粘附及胚胎发育的影响。

• 分子诊断对照： 作为 PCR 或恒温扩增检测盒的阳性对照标准品。

4. 注意事项

• 细胞死亡风险： 由于该菌产氨会导致培养基 pH 迅速升高，一旦变红必须立即转种或冷冻保存，否则细菌会因碱中毒迅速死亡。

• 过滤除菌： 由于其体积极小，可通过 0.45 $\mu$m 滤膜，实验室操作需防范污染。

English Datasheet

1. General Product Information

• Product Name: BioVector® Ureaplasma urealyticum

• Genomic Profile: Highly reduced genome (~0.75-1.0 Mbp), showing extreme dependency on external nutrient sources.

• Morphology: Pleomorphic (spherical to pear-shaped), 0.1-1.0 $\mu$m in diameter; lacks a peptidoglycan cell wall and does not respond to Gram staining.

2. Culture Conditions

• Medium: BioVector® Ureaplasma Medium (Medium 10), enriched with horse/bovine serum, urea, and growth supplements.

• pH Optimization: Initial pH must be set to 6.0 ($\pm 0.2$).

• Environment: 37°C in a 5% $CO_2$ incubator.

• Growth Indicator: Phenol red in the medium shifts from yellow to pink/red as urease activity produces ammonia, raising the pH.

3. Applications

• Antimicrobial Susceptibility Testing (AST): Monitoring resistance trends to macrolides, tetracyclines, and fluoroquinolones.

• Reproductive Medicine: Investigating the impact of Ureaplasma colonization on sperm motility and maternal-fetal health.

• Diagnostic Standardization: Serving as a quantitative positive control for molecular assays (e.g., qPCR, LAMP).

4. Key Usage Notes

• Toxicity Warning: The metabolic byproduct (ammonia) is toxic to the bacteria. Cultures must be harvested or subcultured immediately upon color change to prevent "overgrowth" death.

• Biosafety: Classified as Biosafety Level 2 (BSL-2). Requires stringent aseptic technique and disposal protocols for urogenital pathogens.

注意 / Note: 解脲支原体在固体培养基上形成的菌落极小，通常需要使用低倍显微镜才能观察到典型的“油煎蛋”或颗粒状形态。

Colonies of Ureaplasma urealyticum on agar are microscopic; observation typically requires a low-power microscope to visualize the characteristic "fried-egg" or granular appearance.

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