BioVector® AY-27 大鼠膀胱癌细胞系 / BioVector® AY-27 Rat Bladder Cancer Cell Line

通用定义 / General Definition:

BioVector® AY-27 是一种广泛使用的大鼠膀胱移行细胞癌 (Urothelial/Transitional Cell Carcinoma, TCC) 细胞系。该细胞系最初是通过向 Fischer 344 (F344) 大鼠膀胱内灌注致癌物质（如 $N$-丁基-$N$-(4-羟丁基)亚硝胺，BBN）诱导产生的。AY-27 的核心价值在于其能够在大鼠体内建立原位 (Orthotopic) 膀胱癌模型，高度模拟人类膀胱癌的生长环境、组织学特征以及对药物的反应。它被公认为研究膀胱癌免疫疗法（如 BCG 灌注）、光动力疗法 (PDT) 以及局部化疗药物递送的“金标准”大鼠模型。

BioVector® AY-27 is a widely utilized Rat Bladder Transitional Cell Carcinoma (TCC) cell line. It was originally derived from an $N$-butyl-$N$-(4-hydroxybutyl)nitrosamine (BBN)-induced bladder tumor in Fischer 344 (F344) rats. The primary significance of AY-27 lies in its ability to establish orthotopic bladder cancer models, which closely mimic the microenvironment, histological progression, and therapeutic responses of human bladder cancer. It is recognized as a premier model for investigating intravesical therapies, such as BCG immunotherapy, Photodynamic Therapy (PDT), and localized drug delivery systems.

BioVector® AY-27 技术说明书 (Technical Datasheet)

中文版说明书 (Chinese Datasheet)

1. 产品基本信息

• 产品名称： BioVector® AY-27 大鼠膀胱癌细胞

• 组织来源： F344 大鼠膀胱移行上皮 (Urothelium)

• 生长特性： 贴壁生长

• 细胞形态： 上皮样，通常呈铺路石状排列。

2. 培养条件

• 基础培养基： BioVector® RPMI 1640。

• 血清添加： 10% BioVector® 优质胎牛血清 (FBS)。

• 培养环境： 37 摄氏度，5% $CO_2$。

• 传代比例： 1:3 至 1:6；建议在细胞汇合度达到 80%-90% 时进行传代。

3. 细胞应用

• 原位肿瘤模型： 通过导尿管将 AY-27 细胞灌注至 F344 大鼠膀胱内（通常需预先轻微损伤膀胱黏膜），建立原位癌模型。

• 免疫治疗研究： 评估卡介苗 (BCG) 或细胞因子对膀胱肿瘤微环境的免疫调节作用。

• 药物动力学： 测试经尿道灌注药物（如丝裂霉素、多柔比星）在膀胱壁的渗透性与抗肿瘤效能。

4. 注意事项

• 同源性： 为确保成瘤率，体内实验必须使用 F344 大鼠，以避免免疫排斥。

• 成瘤控制： 该细胞系在体内生长极快，原位接种后需密切通过超声或血尿情况监测肿瘤进展，防止大鼠过早死亡。

English Datasheet

1. General Product Information

• Product Name: BioVector® AY-27 Rat Bladder Cancer Cell Line

• Source: Bladder Urothelium of Fischer 344 (F344) rats

• Growth Properties: Adherent

• Morphology: Epithelial-like; typical cobblestone pattern.

2. Culture Conditions

• Basal Medium: BioVector® RPMI 1640.

• Serum Supplement: 10% BioVector® Fetal Bovine Serum (FBS).

• Incubation: 37°C, 5% $CO_2$.

• Subculturing: 1:3 to 1:6 ratio; subculture at 80%-90% confluence.

3. Applications

• Orthotopic Tumor Modeling: Cells are instilled intravesically into F344 rats (often following a brief chemical or mechanical pre-conditioning of the bladder wall) to study localized cancer growth.

• Immunotherapy Research: A standard model for studying BCG-mediated immune responses and the recruitment of inflammatory cells to the tumor site.

• Photodynamic Therapy (PDT): Widely used to test photosensitizers and laser-based ablation techniques for superficial bladder tumors.

4. Key Usage Notes

• Syngeneic Consistency:Fischer 344 rats must be used for in vivo studies to ensure successful engraftment and prevent immunological rejection of the cells.

• Aggressive Growth: AY-27 exhibits rapid proliferation in vivo. Researchers should perform regular monitoring (e.g., small animal ultrasound) to track tumor volume and ensure animal welfare protocols are met.

注意 / Note: AY-27 细胞在多次传代后可能会降低其在体内的成瘤效率。BioVector® 建议使用低代数细胞进行体内实验，并验证细胞的形态一致性。

AY-27 cells may show a decline in orthotopic engraftment efficiency after extensive passaging. BioVector® recommends utilizing low-passage stocks for in vivo studies and periodically confirming epithelial morphology.

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