BioVector® H80X 抗人绒毛膜促性腺激素 alpha 杂交瘤细胞 / BioVector® H80X Anti-hCG alpha Hybridoma Cell Line

通用定义 / General Definition:BioVector® H80X 是一种专门用于产生单克隆抗体的小鼠杂交瘤细胞系。该细胞系通过将经过人绒毛膜促性腺激素 alpha 亚基 (hCG alpha) 免疫的小鼠脾细胞与小鼠骨髓瘤细胞融合而成。H80X 分泌的单克隆抗体能够特异性识别 hCG 的 alpha 亚基。由于 hCG, LH, FSH 和 TSH 共享相同的 alpha 亚基，该细胞系生产的抗体常用于研究这类糖蛋白激素的共同结构特征，或在双抗体夹心法 ELISA 中作为捕获或检测抗体对的一部分。

BioVector® H80X is a mouse hybridoma cell line dedicated to the production of monoclonal antibodies. It was generated by fusing splenocytes from mice immunized with the alpha subunit of Human Chorionic Gonadotropin (hCG alpha) with a mouse myeloma partner. The monoclonal antibody secreted by H80X specifically recognizes the alpha subunit of hCG. Since hCG, LH, FSH, and TSH all share a common alpha subunit, antibodies from this line are frequently used to study the shared structural motifs of these glycoprotein hormones or serve as part of a capture or detection pair in sandwich ELISA assays.

BioVector® H80X 技术说明书 (Technical Datasheet)

中文版说明书 (Chinese Datasheet)

1. 产品基本信息

• 产品名称: BioVector® H80X 抗 hCG alpha 杂交瘤细胞

• 抗体类型: 单克隆抗体 (mAb)

• 抗原特异性: 人绒毛膜促性腺激素 alpha 亚基 (hCG alpha)

• 宿主背景: Balb/c 小鼠

• 生长特性: 半悬浮生长

• 细胞形态: 典型的杂交瘤形态，圆形，大小均匀

2. 培养条件

• 基础培养基: BioVector® RPMI 1640

• 血清添加: 10% 到 15% BioVector® 优质胎牛血清 (FBS)

• 补充因子: 2 mM L-谷氨酰胺，1 mM 丙酮酸钠

• 培养环境: 37 摄氏度，5% CO2

• 传代比例: 1:3 至 1:5，建议保持细胞密度在 200,000 到 1,000,000 cells/mL 之间

3. 细胞应用

• 抗体生产: 通过体外培养法或小鼠腹水法大规模生产抗 hCG alpha 单克隆抗体。

• 免疫检测开发: 用于开发早孕诊断试剂盒或生殖激素检测试剂。

• 交叉反应研究: 研究 alpha 亚基在不同促性腺激素（如 LH, FSH, TSH）中的保守性。

4. 注意事项

• 分泌稳定性: 杂交瘤细胞在多次传代后可能丢失产生抗体的能力。建议定期通过 ELISA 检测细胞上清液的抗体滴度。

• 抗体分型: 建议在使用前确认该克隆分泌抗体的同种型，以便于后续纯化。

English Datasheet

1. General Product Information

• Product Name: BioVector® H80X Anti-hCG alpha Hybridoma Cell Line

• Antibody Type: Monoclonal Antibody (mAb)

• Specificity: Human Chorionic Gonadotropin alpha subunit (hCG alpha)

• Host Background: Balb/c Mouse

• Growth Properties: Semi-suspension

• Morphology: Typical hybridoma morphology; spherical cells with uniform size

2. Culture Conditions

• Basal Medium: BioVector® RPMI 1640

• Serum Supplement: 10% to 15% BioVector® Fetal Bovine Serum (FBS)

• Supplements: 2 mM L-Glutamine, 1 mM Sodium Pyruvate

• Incubation: 37 degrees Celsius, 5% CO2

• Subculturing: 1:3 to 1:5 ratio; maintain density between 200,000 and 1,000,000 cells/mL

3. Applications

• Antibody Production: Large-scale manufacturing of anti-hCG alpha mAbs via in vitro bioreactors or in vivo mouse ascites methods.

• Diagnostic Assay Development: Core component for pregnancy tests and reproductive hormone screening.

  

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• Structural Biology: Probing the common alpha-subunit epitopes shared by the glycoprotein hormone family.

4. Key Usage Notes

• Stability Monitoring: Hybridoma lines can drift or lose antibody secretion over time. BioVector® recommends frequent titer checks of the culture supernatant via ELISA.

• Isotype Verification: Confirm the immunoglobulin isotype to optimize purification strategies using Protein A or Protein G chromatography.

注意 / Note: H80X 杂交瘤细胞应在生物安全二级 (BSL-2) 条件下操作。在进行大规模抗体生产前，建议先进行单细胞克隆化以确保抗体分泌的一致性。H80X hybridoma cells should be handled under Biosafety Level 2 (BSL-2) conditions. Before large-scale antibody production, BioVector® suggests performing single-cell cloning to ensure the homogeneity and stability of antibody secretion.

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