JHUCS-1 Cell line细胞株-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

JHUCS-1 Cell line细胞株

Comment   Japanese uterine carcinosarcoma. Nude mousetransplantable.  

Animal       human

Subspecies        Japanese

Sex   Female

Age   56 years

Tissue       uterus

Morphology        epithelial-like

Anchoraged        Yes

Medium     (DMEM:HamF12=1:1)+15%FBS

Antibiotics  Free

Growthtemp       37oC

CO2concentration      5%

Passagemethod         0.05% trypsin+0.02% EDTA or0.25% trypsin+0.02% EDTA

Splitratio   1:8 split

Subculturefrequency  1-2 times/week

Lifespan     infinite

Mycoplasma      -

Isozymeanalysis        LD, NP

Chrdistrb  45-49(50):45(1),46(6),47(40),48(2),49(1)

ShortTandem Repeat  OK

Originator  Ishikawa, H. & Yasuda, M. & Yamada, K.

Depositor   Ishikawa, Hiroshi

Medium:

(DMEM:HamF12=1:1)+15%FBS+ 100 U/ml penicillin G sodium (option); 100 µg/mlstreptomycin sulfate (option);

Description:

The cell line designed JHUCS-1 was establishedfrom a carcinosarcoma (malignant mixed mesodermal tumor) of the uterus that wassurgically removed from a 57-year-old Japanese woman. We carefully examined thehistopathology of the original tumor after the cell line was established andnoted differentiation into a neuroendocrine carcinoma within the tumor'sepithelial components. Immunohistochemical staining of the tumorous tissue thathad been heterotransplanted was positive for Leu7. Additionally, secretarygranules were observed in the grafted cells as determined by electronmicroscopy. These results support the existence of neuroendocrine cells withinthe JHUCS-1 cell line.

Reference :  

Yamada, Kyosuke, Tachibana, Toshiaki, Iida, Yasushi,Ueda, Kazu, Misawa, Akihiko, Suzuki, Nagazumi, Takahashi, Hiroyuki, Kato,Hiroyuki, Kimura, Eizo, Yasuda, Makoto, Tanaka, Tadao, Ishikawa, Hiroshi HumCell 2004; 17:139-44.

PubMed ID: 15859159