CS-99 cell line细胞株-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

CS-99 Cells

CatNo.: NTCC503819

Disease     Uterine carcinosarcoma

Speciesof origin         Homo sapiens (Human)

Sex ofcell  Female

Category      Cancercell line

The CS-99cell line was derived from a uterine carcinosarcoma retaining features of thesarcomatous phenotype in vitro. CS-99 cells exhibit a mesenchymal morphologywith predominantly spindle-shaped cells at nonconfluence turning to pleomorphicappearance at confluence. The mesenchymal phenotype was evidencedimmunohistochemically by strong vimentin and moderate SM-actin, which wassimilar to the sarcomatous component of the primary tumor. P53 wasoverexpressed in a subset of CS-99 cells. Epithelial membrane antigen wasmoderately expressed whereas other markers including pan CK, CK 5/6, CK 34,epidermal growth factor receptor, desmin, carcinoembryonic antigen, S100, KIT,ERBB2, and the hormone receptors, estrogen receptor and progesterone receptorrevealed either weak or no specific staining in CS-99 cells. High self-renewalcapacity corresponded to the population doubling time of 23 h in high passage.CS-99 cells were able to develop three-dimensional tumor spheroids in vitro.Cytogenetic analysis and multicolor fluorescence in situ hybridization of CS-99demonstrated an almost stable karyotype including numerical changes +8, +18,and +20 and translocations, amongst others der(1)t(1;2), der(1)t(1;7),der(2)t(2;19), der(5)t(5;8), and der(5)t(5;14). Taken together, the cell lineCS-99 exhibits strong growths dynamics and a complex but stable karyotype inhigher passages, and can be further a useful in vitro model system for studyingtumor biology of carcinosarcomas.

Medium:

DMEM+10%FBS(Gibco)+ 100 U/ml penicillin G sodium(option); 100 µg/ml streptomycin sulfate (option);