KBM5-T315I Cell Line细胞株-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

KBM5-T315I Cell Line

Cat No.: NTCC510625

Disease: Chronic myelogenous leukemia, BCR-ABL1 positive

Species of origin: Homo sapiens

Sex of cell: Female

Category: Cancer cell line

KBM-5 is a leukemic cell line derived from a patient in the blastic phase of chronic myelogenous leukemia. The cells exhibited multiple copies of the

Philadelphia chromosome, and a high level of p210Bcr-Abl kinase activity was detected with rabbit anti-Abl and anti-Bcr (exon 3) peptide antisera.

Use of specific primers and polymerase chain reaction followed by Southern blotting revealed that KBM-5 cells carried a bcr3-ABLII splice junction.

While a normal BCR message was detected, no normal ABL message was found. The cells were phenotypically myeloid with monocytic

differentiation. The high cloning efficiency in semisolid media was independent of the presence of exogenous colony-stimulating factors. In vitro

exposure to induces of differentiation, such as retinoic acid, dimethyl sulfoxide, or hemin, failed to influence the growth rate of the cells and their level

of differentiation. KBM-5 cells are highly resistant to the antiproliferative action of recombinant alpha- and gamma-interferons. Although sensitive to

recombinant tumor necrosis factor alpha, they were completely resistant to natural killer cell action. KBM-5 cells constitutively expressed mRNA for

tumor necrosis factor alpha but not for gamma-interferon, other interleukins, or hematopoietic growth factors. The KBM-5 cells that were transplanted

into SCID mice manifested metastatic potential and tissue invasiveness similar to the way leukemic cells in humans do. This new KBM-5 cell line

represents a helpful model for examining in vitro and in vivo modulation of the growth and properties of leukemic cells by using biological and

chemotherapeutic agents.

Medium: IMDM medium with 10% FBS(Gibco)