pX551腺相关病毒CRISPR/Cas9基因编辑系统载体mecp2启动子-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
- 价 格：¥4950
- 货 号：pX551腺相关病毒CRISPR/Cas9基因编辑系统载体
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
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地址：北京
- 已注册
PX551
(Plasmid 60957)
-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
pX551腺相关病毒CRISPR/Cas9基因编辑系统载体，mecp2启动子
pAAV-pMecp2-SpCas9-spA (AAV-SpCas9). AAV plasmid expressing Cas9 in neurons under control of truncated mecp2 promoter.
Map图谱：
Sequence序列：
>PX551 sequence 7400 bps
cctgcaggcagctgcgcgctcgctcgctcactgaggccgcccgggcaaagcccgggcgtcgggcgacctt
tggtcgcccggcctcagtgagcgagcgagcgcgcagagagggagtggccaactccatcactaggggttcc
tgcggcctctagaaagcttAGCTGAATGGGGTCCGCCTCTTTTCCCTGCCTAAACAGACAGGAACTCCTG
CCAATTGAGGGCGTCACCGCTAAGGCTCCGCCCCAGCCTGGGCTCCACAACCAATGAAGGGTAATCTCGA
CAAAGAGCAAGGGGTGGGGCGCGGGCGCGCAGGTGCAGCAGCACACAGGCTGGTCGGGAGGGCGGGGCGC
GACGTCTGCCGTGCGGGGTCCCGGCATCGGTTGCGCGCaccggtgccaccatgtacccatacgatgttcc
agattacgcttcgccgaagaaaaagcgcaaggtcgaagcgtccgacaagaagtacagcatcggcctggac
atcggcaccaactctgtgggctgggccgtgatcaccgacgagtacaaggtgcccagcaagaaattcaagg
tgctgggcaacaccgaccggcacagcatcaagaagaacctgatcggagccctgctgttcgacagcggcga
aacagccgaggccacccggctgaagagaaccgccagaagaagatacaccagacggaagaaccggatctgc
tatctgcaagagatcttcagcaacgagatggccaaggtggacgacagcttcttccacagactggaagagt
ccttcctggtggaagaggataagaagcacgagcggcaccccatcttcggcaacatcgtggacgaggtggc
ctaccacgagaagtaccccaccatctaccacctgagaaagaaactggtggacagcaccgacaaggccgac
ctgcggctgatctatctggccctggcccacatgatcaagttccggggccacttcctgatcgagggcgacc
tgaaccccgacaacagcgacgtggacaagctgttcatccagctggtgcagacctacaaccagctgttcga
ggaaaaccccatcaacgccagcggcgtggacgccaaggccatcctgtctgccagactgagcaagagcaga
cggctggaaaatctgatcgcccagctgcccggcgagaagaagaatggcctgttcggcaacctgattgccc
tgagcctgggcctgacccccaacttcaagagcaacttcgacctggccgaggatgccaaactgcagctgag
caaggacacctacgacgacgacctggacaacctgctggcccagatcggcgaccagtacgccgacctgttt
ctggccgccaagaacctgtccgacgccatcctgctgagcgacatcctgagagtgaacaccgagatcacca
aggcccccctgagcgcctctatgatcaagagatacgacgagcaccaccaggacctgaccctgctgaaagc
tctcgtgcggcagcagctgcctgagaagtacaaagagattttcttcgaccagagcaagaacggctacgcc
ggctacattgacggcggagccagccaggaagagttctacaagttcatcaagcccatcctggaaaagatgg
acggcaccgaggaactgctcgtgaagctgaacagagaggacctgctgcggaagcagcggaccttcgacaa
cggcagcatcccccaccagatccacctgggagagctgcacgccattctgcggcggcaggaagatttttac
ccattcctgaaggacaaccgggaaaagatcgagaagatcctgaccttccgcatcccctactacgtgggcc
ctctggccaggggaaacagcagattcgcctggatgaccagaaagagcgaggaaaccatcaccccctggaa
cttcgaggaagtggtggacaagggcgcttccgcccagagcttcatcgagcggatgaccaacttcgataag
aacctgcccaacgagaaggtgctgcccaagcacagcctgctgtacgagtacttcaccgtgtataacgagc
tgaccaaagtgaaatacgtgaccgagggaatgagaaagcccgccttcctgagcggcgagcagaaaaaggc
catcgtggacctgctgttcaagaccaaccggaaagtgaccgtgaagcagctgaaagaggactacttcaag
aaaatcgagtgcttcgactccgtggaaatctccggcgtggaagatcggttcaacgcctccctgggcacat
accacgatctgctgaaaattatcaaggacaaggacttcctggacaatgaggaaaacgaggacattctgga
agatatcgtgctgaccctgacactgtttgaggacagagagatgatcgaggaacggctgaaaacctatgcc
cacctgttcgacgacaaagtgatgaagcagctgaagcggcggagatacaccggctggggcaggctgagcc
ggaagctgatcaacggcatccgggacaagcagtccggcaagacaatcctggatttcctgaagtccgacgg
cttcgccaacagaaacttcatgcagctgatccacgacgacagcctgacctttaaagaggacatccagaaa
gcccaggtgtccggccagggcgatagcctgcacgagcacattgccaatctggccggcagccccgccatta
agaagggcatcctgcagacagtgaaggtggtggacgagctcgtgaaagtgatgggccggcacaagcccga
gaacatcgtgatcgaaatggccagagagaaccagaccacccagaagggacagaagaacagccgcgagaga
atgaagcggatcgaagagggcatcaaagagctgggcagccagatcctgaaagaacaccccgtggaaaaca
cccagctgcagaacgagaagctgtacctgtactacctgcagaatgggcgggatatgtacgtggaccagga
actggacatcaaccggctgtccgactacgatgtggaccatatcgtgcctcagagctttctgaaggacgac
tccatcgacaacaaggtgctgaccagaagcgacaagaaccggggcaagagcgacaacgtgccctccgaag
aggtcgtgaagaagatgaagaactactggcggcagctgctgaacgccaagctgattacccagagaaagtt
cgacaatctgaccaaggccgagagaggcggcctgagcgaactggataaggccggcttcatcaagagacag
ctggtggaaacccggcagatcacaaagcacgtggcacagatcctggactcccggatgaacactaagtacg
acgagaatgacaagctgatccgggaagtgaaagtgatcaccctgaagtccaagctggtgtccgatttccg
gaaggatttccagttttacaaagtgcgcgagatcaacaactaccaccacgcccacgacgcctacctgaac
gccgtcgtgggaaccgccctgatcaaaaagtaccctaagctggaaagcgagttcgtgtacggcgactaca
aggtgtacgacgtgcggaagatgatcgccaagagcgagcaggaaatcggcaaggctaccgccaagtactt
cttctacagcaacatcatgaactttttcaagaccgagattaccctggccaacggcgagatccggaagcgg
cctctgatcgagacaaacggcgaaaccggggagatcgtgtgggataagggccgggattttgccaccgtgc
ggaaagtgctgagcatgccccaagtgaatatcgtgaaaaagaccgaggtgcagacaggcggcttcagcaa
agagtctatcctgcccaagaggaacagcgataagctgatcgccagaaagaaggactgggaccctaagaag
tacggcggcttcgacagccccaccgtggcctattctgtgctggtggtggccaaagtggaaaagggcaagt
ccaagaaactgaagagtgtgaaagagctgctggggatcaccatcatggaaagaagcagcttcgagaagaa
tcccatcgactttctggaagccaagggctacaaagaagtgaaaaaggacctgatcatcaagctgcctaag
tactccctgttcgagctggaaaacggccggaagagaatgctggcctctgccggcgaactgcagaagggaa
acgaactggccctgccctccaaatatgtgaacttcctgtacctggccagccactatgagaagctgaaggg
ctcccccgaggataatgagcagaaacagctgtttgtggaacagcacaagcactacctggacgagatcatc
gagcagatcagcgagttctccaagagagtgatcctggccgacgctaatctggacaaagtgctgtccgcct
acaacaagcaccgggataagcccatcagagagcaggccgagaatatcatccacctgtttaccctgaccaa
tctgggagcccctgccgccttcaagtactttgacaccaccatcgaccggaagaggtacaccagcaccaaa
gaggtgctggacgccaccctgatccaccagagcatcaccggcctgtacgagacacggatcgacctgtctc
agctgggaggcgacagccccaagaagaagagaaaggtggaggccagctaagaattcAATAAAAGATCTTT
ATTTTCATTAGATCTGTGTGTTGGTTTTTTGTGTgcggccgcaggaacccctagtgatggagttggccac
tccctctctgcgcgctcgctcgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcc
cgggcggcctcagtgagcgagcgagcgcgcagctgcctgcaggggcgcctgatgcggtattttctcctta
cgcatctgtgcggtatttcacaccgcatacgtcaaagcaaccatagtacgcgccctgtagcggcgcatta
agcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctt
tcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccc
tttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgatttgggtgatggttcacgt
agtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggac
tcttgttccaaactggaacaacactcaaccctatctcgggctattcttttgatttataagggattttgcc
gatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatatta
acgtttacaattttatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgac
acccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgt
gaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggc
ctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcactt
ttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcat
gagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgt
gtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaag
taaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagat
ccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcg
gtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttgg
ttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgc
cataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaacc
gcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagcca
taccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactgg
cgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggacca
cttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtggaagcc
gcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggag
tcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaa
ctgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatct
aggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtc
agaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaa
acaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaagg
taactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccactt
caagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggc
gataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaa
cggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtga
gctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcgga
acaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgcc
acctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaa
cgcggcctttttacggttcctggccttttgctggccttttgctcacatgt
- Vector backbonepAAV(Search Vector Database)
Backbone size w/o insert (bp)2905
Total vector size (bp)7400
- Vector typeMammalian Expression, AAV, CRISPR
GROWTH IN BACTERIA
- Bacterial Resistance(s)Ampicillin
- Growth Temperature37°C
- Growth Strain(s)Stbl3
- Copy numberHigh Copy
GENE/INSERT
- Gene/Insert nameSpCas9
- Alt nameS. pyogenes Cas9 nuclease
- Alt nameCas9
- SpeciesSynthetic
- Insert Size (bp)4200
PromoterpMecp2
Tag / Fusion Protein
HA (N terminal on insert)
CLONING INFORMATION
Cloning methodRestriction Enzyme
5′ cloning siteAgeI (not destroyed)
3′ cloning siteEcoRI (not destroyed)
5′ sequencing primerAATGGGGTCCGCCTCTTTTCC
3′ sequencing primercttagctggcctccacctttctcttc
- 公告/新闻
