NCFM Lactobacillus acidophilus嗜酸乳杆菌菌株-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

• 中文名称：嗜酸乳杆菌

• 拉丁名称：Lactobacillus acidophilus

  
  

• 收藏时间：2008/2/22

• 原始编号：NCFM

• 原产国：丹麦

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• 模式菌株：非模式菌株

• 主要用途：研究；教学；生产

• 特征特性：发酵纤维二糖、果糖、半乳糖、葡萄糖、乳糖、麦芽糖、甘露糖、蔗糖呈阳性反应；兼性厌氧。 能产生功能性物质共轭亚油酸（CLA），生产强化CLA的功能性乳制品。

• 具体用途：制作功能性酸奶及其相关乳制品

• 生物危害程度：四类

  
  

• 培养基名称：MRS培养基

• 培养基成分：酪蛋白胨 10.0g，牛肉膏 10.0g，酵母粉 5.0g，葡萄糖 5.0g，乙酸钠 5.0g，柠檬酸二铵 2.0g，Tween 80 1.0g，K2HPO4 2.0g，MgSO4.7H2O 0.2g，MnSO4.H2O 0.05g，CaCO3 20.0g，琼脂 15.0g，蒸馏水 1.0L，pH6.8。
  *培养时pH需调至5.0.

• 培养温度：37℃

• 需氧类型：厌氧

• 分离基物：商业化酸奶发酵剂

  
  

• 保存方法：真空冷冻干燥法

• 保藏机构：BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

Description

L. acidophilus NCFM has beenconfirmed as a true type A1 L. acidophilus using phenotypic and genotypicmethods, including 16S rRNA gene sequencing and hybridisation to aspecies-specific probe and whole genome sequencing. Probiotics are livemicroorganisms, which, when administered in adequate amounts, confer a healthbenefit on the host. FAO/WHO 2001

Figure1. Scanning electron micrograph of L. acidophilus NCFM.

L.acidophilus NCFM was first isolated from a human source in the early 1970s. Thename NCFM is derived from “North Carolina Food Microbiology”, the researchlaboratory at North Carolina State University (NCSU) where this successfulisolation took place.

GENOMICS

L.acidophilus NCFM is the only strain of this species for which the genome hasbeen sequenced and annotated. Genomic work has identified several regionsimportant in probiotic functionality and which support the role of L.acidophilus NCFM in maintaining or restoring gastrointestinal wellbeing.Included in this are genes involved in bacteriocin production, sugar andprebiotic metabolism, adherence to human cell lines, lactose metabolism, andtolerance to physiologically relevant stresses, including acid and bile.Genomic reconstruction of metabolic pathways reflects the adaptation of L.acidophilus NCFM to the gastrointestinal environment. Targeted gene disruptionsare being evaluated to help establish probiotic functionality in relation tospecific gene products and validate and support functional probiotic targets.Genomic information is also used to develop strain identity methods and improveindustrial processes.

Name:Lactobacillus acidophilus

Original No.:NCFM

Supplier: BioVector NTCC Inc. www.biovector.net

Countryof Origin:Denmark

Application:Production of functional Yoghurt and related dairy products

AdditionalInformation:Produces conjugated linoleic acid (CLA)

Medium:Casein Peptone 10.0 g, Beef extract 10.0 g, Yeast extract 5.0 g, Glucose 5.0 g, Sodiumacetate 5.0 g, Ammonium citrate dibasic 2.0 g, Tween-80 1.0 g, K2HPO4 2.0 g,MgSO4?H2O 0.2 g, MnSO4稨2O 0.05 g, CaCO3 20.0 g, Agar 15.0 g, Distilled water1.0 L, pH 6.8.

Growth Conditions: Anaerobic,37℃

Biosafety Level:1