240E-W2 Cell Line细胞株及完全培养基Oryctolagus cuniculus (Rabbit)来源-BioVector NTCC细胞保藏中心
- 价 格：¥5989860
- 货 号：CVCL_Z069
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作QQ:1843439339 (微信同号)
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240E-W2 Cell Line细胞株及完全培养基Oryctolagus cuniculus (Rabbit)来源-BioVector NTCC细胞保藏中心
240E-W2 cell line
Cat No.: NTCC595101
Cell line name 240E-W2
Comments
Group: Hybridoma fusion partner cell line.
Selected for resistance to: 8-azaguanine.
Disease
Rabbit plasmacytoma
Species of origin Oryctolagus cuniculus (Rabbit)
Hierarchy
Parent: (240E-W)
Category
Cancer cell line
Publications
Patent=US7429487
Pytela R., Zhu W., Ke Y., Qian Q., Au H.C.
Fusion partner for production of monoclonal rabbit antibodies.
Patent number US7429487, 30-Sep-2008
240E-W2 cell line doesn't carry an endogenous heavy chain gene.
Cell Fusion
Fusions were performed using conventional methodology. 1.5−3×108 lymphocytes from an immunized rabbit
and the fusion partner cells were mixed at a ratio of 2:1 with PEG 4000 at (Sigma P7181) 37° C. in serum-free
medium. The cells were transferred into 96-well cell culture plates at approximately 1×105 lymphocytes per well
in 1640 RPMI medium with 15% FBS (or FCS), incubated in a CO2 incubator. After 48 hr HAT medium (Sigma
H0262) was added. Hybridoma colonies were ready for screening in 3-5 weeks. Medium was changed 2-3
times before screening by removing old medium and add fresh medium. Supernatants were tested for the
presence of antibody, specific for the immunogen, by ELISA. Western blot analysis was used as a secondary
screening assay. The hybridomas were sub-cloned by limit dilution. The fusion partner 240E-W2 at 2×104 cells
per well was used as feeder cells.
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