pIC333转座子载体mini-Tn10转座子质粒枯草芽孢杆菌转座子载体-BioVector NTCC Inc.

pIC333 plasmid

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

Description

An improved mini-Tn10 deliveryplasmid pIC333, 7 kb, contains (i) a 2.4-kb mini-Tn10 containing a ColEl originand an SPC resistance gene, (ii) an ERY resistance gene, (iii) athermosensitive origin of replication for gram-positive hosts (inactive attemperatures higher than 35°C) (8), and (iv) a Tn10 transposase gene withmutations previously shown to decrease the target specificity of transpositionin E. coli. Experiments showed that pIC333 is an efficient mini-Tn10 delivery vector in B. subtilis.