EM-3人急变期慢性粒细胞白血病细胞株-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

EM-3人急变期慢性粒细胞白血病细胞株

EM-3 Cell Line

CatNo.: NTCC590134

Cell line:

EM-3

Species:

human (Homo sapiens)

Cell type:

chronic myeloid leukemia in blast crisis

Origin:

established from the bone marrow of a 5-year-oldCaucasian girl with Philadelphia chromosome-positive CML (chronic myeloidleukemia) in second relapse in 1980 (after bone marrow transplantation day+47); sister cell line of EM-2 (DSM ACC 135)

Reference(s):

14488,14656,14883

Biosafety level:

1

Permissions and restrictions:

A

CellCulture Data:

Morphology:

single, round to oval cells in suspension;image;image

Medium:

90% RPMI 1640 + 10% h.i. FBS

Subculture:

maintain at 0.5-1.5 x 10⁶cells/ml; subculture at a 1:2 to 1:3 dilution every 48hours; seed out at ca. 0.5 x 10⁶cells/ml

Incubation:

at 37 °C with 5% CO₂

Doubling time:

ca. 40 hours

Harvest:

grows up to about 2 x 10⁶cells/ml

Storage:

frozen with 70% medium, 20% FBS, 10% DMSO

Scientific Data:

Mycoplasma:

negative in DAPI, microbiological culture, RNAhybridization, PCR assays

Immunology:

CD3 -, CD4 -, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +,HLA-DR -

Fingerprint:

same DNA profile as EM-2 using multiplex PCR ofminisatellite markers

Species:

confirmed as human with IEF of AST, MDH, NP

Cytogenetics:

human hypertriploid karyotype - 73(69-77)<3n>X, -X,-X, t(9;22)(q34;q11), i(17q)

Molec. Genetics:

expression of fusion gene BCR-ABL1 e14-a2 (b3-a2)confirmed by RT-PCR

Viruses:

ELISA: reverse transcriptase negative; PCR: EBV -, HBV -,HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -