p413 MET25，p423 MET25，p414 MET25，p424 MET25，p415 MET25，p425 MET25，p416 MET25，p426 MET25，p413 GAL1酵母高拷贝表达载体-BioVector

p413 MET25，p423 MET25，p414 MET25，p424 MET25，p415 MET25，p425 MET25，p416 MET25，p426 MET25，p413 GAL1，p423 GAL1，p414 GAL1，p424 GAL1，p415 GAL1，p425 GAL1，p416 GAL1，p426 GAL1，p413 GALL，p423 GALL，p414 GALL，p424 GALL，p415 GALL，p425 GALL，p416 GALL，p426 GALL，p413 GALS，p423 GALS，p414 GALS，p424 GALS，p415 GALS，p425 GALS，p416 GALS，p426 GALS酵母高拷贝表达载体

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net

Product Information for NTCC 87670

NTCC Catalog No. 87670  

Designation: High Copy Yeast Expression Vectors

Description: A set of 32 yeast expression vectors (ATCC 87318 - 87349) differing in promoter (MET25, GAL1, GALL, GALS),

selection marker (HIS3, TRP1, LEU2, URA3) and replicon (CEN6/ARSH4, 2 micron).

CONTENTS OF PLATE LABELLED ATCC#87670

NTCC#s:

1 2 3 4 5 6 7 8 9 10 11 12

A 87318 87319 87320 87321 87322 87323 87324 87325 87326 87327 87328 87329

B 87330 87331 87332 87333 87334 87335 87336 EMPTY 87338 87339 87340 87341

C 87342 87343 87344 87345 87346 87347 87348 87349

NTCC# Vector name Promoter Replicon Terminator Marker Well

87318 p413 MET25 MET25 CEN6/ARSH4 CYC1 HIS3 a1

87319 p423 MET25 MET25 2 micron CYC1 HIS3 a2

87320 p414 MET25 MET25 CEN6/ARSH4 CYC1 TRP1 a3

87321 p424 MET25 MET25 2 micron CYC1 TRP1 a4

87322 p415 MET25 MET25 CEN6/ARSH4 CYC1 LEU2 a5

87323 p425 MET25 MET25 2 micron CYC1 LEU2 a6

87324 p416 MET25 MET25 CEN6/ARSH4 CYC1 URA3 a7

87325 p426 MET25 MET25 2 micron CYC1 URA3 a8

87326 p413 GAL1 GAL1 CEN6/ARSH4 CYC1 HIS3 a9

87327 p423 GAL1 GAL1 2 micron CYC1 HIS3 a10

87328 p414 GAL1 GAL1 CEN6/ARSH4 CYC1 TRP1 a11

87329 p424 GAL1 GAL1 2 micron CYC1 TRP1 a12

87330 p415 GAL1 GAL1 CEN6/ARSH4 CYC1 LEU2 b1

87331 p425 GAL1 GAL1 2 micron CYC1 LEU2 b2

87332 p416 GAL1 GAL1 CEN6/ARSH4 CYC1 URA3 b3

87333 p426 GAL1 GAL1 2 micron CYC1 URA3 b4

87334 p413 GALL GALL CEN6/ARSH4 CYC1 HIS3 b5

87335 p423 GALL GALL 2 micron CYC1 HIS3 b6

87336 p414 GALL GALL CEN6/ARSH4 CYC1 TRP1 b7

EMPTY EMPTY EMPTY EMPTY EMPTY EMPTY b8

87338 p415 GALL GALL CEN6/ARSH4 CYC1 LEU2 b9

87339 p425 GALL GALL 2 micron CYC1 LEU2 b10

87340 p416 GALL GALL CEN6/ARSH4 CYC1 URA3 b11

87341 p426 GALL GALL 2 micron CYC1 URA3 b12

87342 p413 GALS GALS CEN6/ARSH4 CYC1 HIS3 c1

87343 p423 GALS GALS 2 micron CYC1 HIS3 c2

87344 p414 GALS GALS CEN6/ARSH4 CYC1 TRP1 c3

87345 p424 GALS GALS 2 micron CYC1 TRP1 c4

87346 p415 GALS GALS CEN6/ARSH4 CYC1 LEU2 c5

87347 p425 GALS GALS 2 micron CYC1 LEU2 c6

87348 p416 GALS GALS CEN6/ARSH4 CYC1 URA3 c7

87349 p426 GALS GALS 2 micron CYC1 URA3 c8

Distribution host: Escherichia coli HB101 (ATCC 33694)

Suggested host for expression: Saccharomyces cerevisiae YPH499 (ATCC 76625)

Host range: Sacharomyces cerevisiae, Escherichia coli

Distributed: frozen microtiter array

Medium composition: LB Medium with 50 mcg/ml ampicillin

Growth temperature: 370C

DEPOSITOR(S):

Martin Funk, MediGene GmbH, Martinsried/Muenchen, Germany

REFERENCE:

Nucleic Acids Res. 22: 5767-5768, 1994

ATCC #87318, p413 MET25

Description: Low copy number shuttle expression vector. Expression from the O-acetyl homoserine sulfhydrylase (MET25) promoter

is repressed when cells are grown in the presence of methionine.

- Nucleic Acids Res. 22: 5767-5768, 1994

Vector size (kb): 5.577

Markers: ampR, HIS3

Cloning sites: XbaI SpeI BamHI SmaI EcoRI EcoRV ClaI SalI XhoI

Construction: pRS413, MET25 promoter

Features (with orientation and position when available):

marker(s): HIS3, ->, 200-1395

terminator: CYC1, ->, 2077-2337

MCS: XhoI...XbaI, ->, 2337-2400

promoter for expression: MET25, <-, 2401-2782

marker(s): ampR, <-, 4029-4959

centromere: CEN6/ARSH4, ->, 5063-5577

Notes: Restriction digests of the clone give the following sizes (kb): SacI/XbaI--5.2, 0.4; KpnI--4.6, 1.0; PstI--4.4, 1.2. - ATCC staff

ATCC 87319, p423 MET25

Description: High copy number shuttle expression vector. Expression from the O-acetyl homoserine sulfhydrylase (MET25)

promoter is repressed when cells are grown in the presence of methionine.

- Nucleic Acids Res. 22: 5767-5768, 1994

Vector size (kb): 6.410

Markers: ampR, HIS3

Cloning sites: SpeI BamHI SmaI EcoRI EcoRV ClaI SalI XhoI

Construction: pRS423, MET25 promoter

Features (with orientation and position when available):

marker(s): HIS3, ->, 200-1395

terminator: CYC1, ->, 2077-2337

MCS: XhoI...SpeI, ->, 2337-2394

promoter for expression: MET25, <-, 2401-2782

marker(s): ampR, <-, 3933-4863

replicon: 2 micron, ->, 4963-6303

Notes: Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.2, 2.8, 0.4; KpnI--5.4, 1.0; PstI--5.2, 1.2. - ATCC

staff

ATCC 87320, p414 MET25

Description: Low copy number shuttle expression vector. Expression from the O-acetyl homoserine sulfhydrylase (MET25) promoter

is repressed when cells are grown in the presence of methionine.

- Nucleic Acids Res. 22: 5767-5768, 1994

Vector size (kb): 5.395

Markers: ampR, TRP1

Product Information Sheet for ATCC®87670

American Type Culture Collection 800-638-6597 or 703-365-2700

P.O. Box 1549 Fax: 703-365-2750

Manassas, VA 20108 USA E-mail: tech@atcc.org

www.atcc.org Or contact your local distributor.

- 3 -

Cloning sites: SpeI BamHI SmaI PstI EcoRI ClaI SalI XhoI

Construction: pRS414, MET25 promoter

Features (with orientation and position when available):

marker(s): TRP1, ->, 200-1213

terminator: CYC1, ->, 1895-2155

MCS: XhoI-SpeI, ->, 2155-2212

promoter for expression: MET25, <-, 2219-2600

marker(s): ampR, <-, 3847-4777

centromere: CEN6/ARSH4, ->, 4881-5395

Notes: Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.3,1.7, 0.4; EcoRV--4.0, 1.4; HindIII--4.2, 1.2. -

ATCC staff

ATCC 87321, p424 MET25

Description: High copy number shuttle expression vector. Expression from the O-acetyl homoserine sulfhydrylase (MET25) promoter

is repressed when cells are grown in the presence of methionine.

- Nucleic Acids Res. 22: 5767-5768, 1994

Vector size(kb): 6.228

Markers: ampR, TRP1

Cloning sites: SpeI BamHI SmaI PstI EcoRI ClaI SalI XhoI

Construction: pRS424, MET25 promoter

Features (with orientation and position when available):

marker(s): TRP1, ->, 200-1213

terminator: CYC1, ->, 1895-2155

MCS: XhoI...SpeI, ->, 2155-2212

promoter for expression: MET25, <-, 2219-2600

marker(s): ampR, <-, 3751-4681

replicon: 2 micron, ->, 4781-6121

Notes: Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.3, 1.7, 1.0, 0.4; EcoRV--5.0, 1.4; HindIII--5.2, 1.2.

- ATCC staff

ATCC 87322, p415 MET 25

Description: Low copy number shuttle expression vector. Expression from the O-acetyl homoserine sulfhydrylase (MET25) promoter

is repressed when cells are grown in the presence of methionine.

- Nucleic Acids Res. 22: 5767-5768, 1994

Vector size (kb): 6.628

Markers: ampR, LEU2

Cloning sites: XbaI SpeI BamHI SmaI PstI HindIII SalI XhoI

Construction: pRS415, MET25 promoter

Features (with orientation and position when available):

marker(s): LEU2, ->, 200-2446

terminator: CYC1, ->, 3128-3388

MCS: XhoI...SpeI, ->, 3388-3445

promoter for expression: MET25, <-, 3452-3833

marker(s): ampR, <-, 5080-6010

centromere: CEN6/ARSH4, ->, 6114-6628

Notes:Restriction digests of the clone give the following sizes (kb): SacI/XbaI--6.4, 0.4; EcoRI--4.2, 2.3; EcoRV--4.0, 2.4. - ATCC

staff

ATCC 87323, p425 MET25

Description: High copy number shuttle expression vector. Expression from the O-acetyl homoserine sulfhydrylase (MET25)

promoter is repressed when cells are grown in the presence of methionine.

- Nucleic Acids Res. 22: 5767-5768, 1994

Vector size (kb): 7.461

Markers: ampR, LEU2

Product Information Sheet for ATCC®87670

American Type Culture Collection 800-638-6597 or 703-365-2700

P.O. Box 1549 Fax: 703-365-2750

Manassas, VA 20108 USA E-mail: tech@atcc.org

www.atcc.org Or contact your local distributor.

- 4 -

Cloning sites: SpeI BamHI SmaI PstI HindIII SalI XhoI

Construction: pRS425, MET25 promoter

Features (with orientation and position when available):

marker(s): LEU2, ->, 200-2446

terminator: CYC1, ->, 3128-3388

MCS: XhoI...SpeI, ->, 3388-3445

promoter for expression: MET25, <-, 3452-3833

marker(s): ampR, <-, 4984-5914

replicon: 2 micron, ->, 6014-7354

Notes: Restriction digests of the clone give the following sizes (kb): SacI/XbaI--4.0, 3.0, 0.4; EcoRI--5.2, 2.3; EcoRV--5.1, 2.4.

- ATCC staff

ATCC 87324, p416 MET25

Description: Low copy number shuttle expression vector. Expression from the O-acetyl homoserine sulfhydrylase (MET25) promoter

is repressed when cells are grown in the presence of methionine.

- Nucleic Acids Res. 22: 5767-5768, 1994

Vector size (kb): 5.505

Markers: ampR, URA3

Cloning sites: XbaI SpeI BamHI SmaI EcoRI HindIII ClaI SalI XhoI

Construction: pRS416, MET25 promoter

Features (with orientation and position when available):

marker(s): URA3, ->, 200-1323

terminator: CYC1, ->, 2005-2265

MCS: XhoI...XbaI, ->, 2265-2328

promoter for expression: MET25, <-, 2329-2710

marker(s): ampR, <-, 3957-4887

centromere: CEN6/ARSH4, ->, 4991-5505

Notes: Restriction digests of the clone give the following sizes (kb): SacI/XbaI--5.2,0.4; EcoRV--3.8, 1.7; PstI--3.6, 1.9.

- ATCC staff

ATCC 87325, p426 MET25

Description: High copy number shuttle expression vector. Expression from the O-acetyl homoserine sulfhydrylase (MET25)

promoter is repressed when cells are grown in the presence of methionine.

- Nucleic Acids Res. 22: 5767-5768, 1994

Vector size (kb): 6.338

Markers: ampR, URA3

Cloning sites: SpeI BamHI SmaI EcoRI HindIII ClaI SalI XhoI

Construction: pRS426 (ATCC 77107), MET25 promoter

Features (with orientation and position when available):

marker(s): URA3, ->, 200-1300

terminator: CYC1, ->, 2004-2264

MCS: XhoI...SpeI, ->, 2265-2322

promoter for expression: MET25, <-, 2339-2720

marker(s): ampR, <-, 3838-4768

replicon: 2 micron, ->, 4868-6208

Notes: Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.2, 2.8, 0.4; EcoRV--4.7, 1.7; PstI--4.5, 1.9.

- ATCC staff

ATCC 87326, p413 GAL1

Description: Low copy number shuttle expression vector. Expression from the galactokinase (GAL1) promoter is tightly repressed by

glucose and is strongly induced by galactose.

- Nucleic Acids Res. 22: 5767-5768, 1994

Vector size ( kb): 5.656

Markers: ampR, HIS3

Product Information Sheet for ATCC®87670

American Type Culture Collection 800-638-6597 or 703-365-2700

P.O. Box 1549 Fax: 703-365-2750

Manassas, VA 20108 USA E-mail: tech@atcc.org

www.atcc.org Or contact your local distributor.

- 5 -

Cloning sites: XbaI SpeI BamHI SmaI EcoRI EcoRV ClaI SalI XhoI

Construction: pRS413, GAL1 promoter

Features (with orientation and position when available):

marker(s): HIS3, ->, 200-1395

terminator: CYC1, ->, 2077-2337

MCS: XhoI...XbaI, ->, 2337-2400

promoter for expression: GAL1, <-, 2401-2861

marker(s): ampR, <-, 4108-5038

centromere: CEN6/ARSH4, ->, 5142-5656

Notes: Restriction digests of the clone give the following sizes (kb): SacI/XbaI--5.3, 0.45; KpnI--4.7, 1.0; PstI--4.5, 1.2.

- ATCC staff

ATCC 87327, p423 GAL1

Description: High copy number shuttle expression vector. Expression from the galactokinase (GAL1) promoter is tightly repressed by

glucose and is strongly induced by galactose.

- Nucleic Acids Res. 22: 5767-5768, 1994

Vector size (kb): 6.489

Markers: ampR, HIS3

Cloning sites: SpeI BamHI SmaI EcoRI EcoRV ClaI SalI XhoI

Construction: pRS423, GAL1 promoter

Features (with orientation and position when available):

marker(s): HIS3, ->, 200-1395

terminator: CYC1, ->, 2077-2337

MCS: XhoI...SpeI, ->, 2337-2394

promoter for expression: GAL1, <-, 2401-2861

marker(s): ampR, <-, 4012-4942

replicon: 2 micron, ->, 5042-6382

Notes: Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.2, 2.8, 0.45; KpnI--5.5, 1.0; PstI--5.3, 1.2.

- ATCC staff

ATCC 87328, p414 GAL1

Description: Low copy number shuttle expression vector. Expression from the galactokinase (GAL1) promoter is tightly repressed by

glucose and is strongly induced by galactose.

- Nucleic Acids Res. 22: 5767-5768, 1994

Vector size (kb): 5.474

Markers: ampR, TRP1

Cloning sites: SpeI BamHI SmaI PstI EcoRI ClaI SalI XhoI

Construction: pRS414, GAL1 promoter

Features (with orientation and position when available):

marker(s): TRP1, ->, 200-1213

terminator: CYC1, ->, 1895-2155

MCS: XhoI...SpeI, ->, 2155-2212

promoter for expression: GAL1, <-, 2219-2679

marker(s): ampR, <-, 3926-4856

centromere: CEN6/ARSH4, ->, 4960-5474

Notes: Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.3,1.8, 0.5; EcoRV--4.2, 1.5; HindIII--4.5, 1.3.

- ATCC staff

ATCC 87329, p424 GAL1

Description: High copy number shuttle expression vector. Expression from the galactokinase (GAL1) promoter is tightly repressed by

glucose and is strongly induced by galactose.

- Nucleic Acids Res. 22: 5767-5768, 1994

Vector size (kb): 6.307

Markers: ampR, TRP1

Product Information Sheet for ATCC®87670

American Type Culture Collection 800-638-6597 or 703-365-2700

P.O. Box 1549 Fax: 703-365-2750

Manassas, VA 20108 USA E-mail: tech@atcc.org

www.atcc.org Or contact your local distributor.

- 6 -

Cloning sites: SpeI BamHI SmaI PstI EcoRI ClaI SalI XhoI

Construction: pRS424, GAL1 promoter

Features (with orientation and position when available):

marker(s): TRP1, ->, 200-1213

terminator: CYC1, ->, 1895-2155

MCS: XhoI...SpeI, ->, 2155-2212

promoter for expression: GAL1, <-, 2219-2679

marker(s): ampR, <-, 3820-4760

replicon: 2 micron, ->, 4860-6200

Notes: Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.2, 1.7, 1.0, 0.45; EcoRI--6.3; BamHI--6.3.

- ATCC staff

ATCC 87330, p415 GAL1

Description:

Low copy number shuttle expression vector.Expression from the galactokinase (GAL1) promoter is tightly repressed by

glucose and is strongly induced by galactose.

- Nucleic Acids Res. 22: 5767-5768, 1994

Vector size(kb): 6.707

Markers: ampR, LEU2

Cloning sites: XbaI SpeI BamHI SmaI PstI HindIII SalI XhoI

Construction: pRS415, GAL1 promoter

Features (with orientation and position when available):

marker(s): LEU2, ->, 200-2446

terminator: CYC1, ->, 3128-3388

MCS: XhoI...XbaI, ->, 3388-3451

promoter for expression: GAL1, <-, 3452-3912

marker(s): ampR, <-, 5159-6089

centromere: CEN6/ARSH4, ->, 6193-6707

Notes: Restriction digests of the clone give the following sizes (kb): SacI/XbaI--6.3,0.5; EcoRI--4.3, 2.3; BamHI--4.7, 1.7.

- ATCC staff

ATCC 87331, p425 GAL1

Description: High copy number shuttle expression vector. Expression from the galactokinase (GAL1) promoter is tightly repressed by

glucose and is strongly induced by galactose.

- Nucleic Acids Res. 22: 5767-5768, 1994

Vector size (kb): 7.540

Markers: ampR, LEU2

Cloning sites: SpeI BamHI SmaI PstI HindIII SalI XhoI

Construction: pRS425, GAL1 promoter

Features (with orientation and position when available):

marker(s): LEU2, ->, 200-2446

terminator: CYC1, ->, 3128-3388

MCS: XhoI...SpeI, ->, 3388-3445

promoter for expression: GAL1, <-, 3452-3912

marker(s): ampR, <-, 5063-5993

replicon: 2 micron, ->, 6093-7433

Notes: Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.9,3.2, 0.5; EcoRI--5.1, 2.4; KpnI--5.8, 1.7.

- ATCC staff

ATCC 87332, p416 GAL1

Description: Low copy number shuttle expression vector. Expression from the galactokinase (GAL1) promoter is tightly repressed by

glucose and is strongly induced by galactose.

- Nucleic Acids Res. 22: 5767-5768, 1994

Vector size (kb): 5.584

Product Information Sheet for ATCC®87670

American Type Culture Collection 800-638-6597 or 703-365-2700

P.O. Box 1549 Fax: 703-365-2750

Manassas, VA 20108 USA E-mail: tech@atcc.org

www.atcc.org Or contact your local distributor.

- 7 -

Markers: ampR, URA3

Cloning sites: XbaI SpeI BamHI SmaI EcoRI HindIII ClaI SalI XhoI

Construction: pRS416, GAL1 promoter

Features (with orientation and position when available):

marker(s): URA3, ->, 200-1323

terminator: CYC1, ->, 2005-2265

MCS: XhoI...XbaI, ->, 2265-2328

promoter for expression: GAL1, <-, 2329-2789

marker(s): ampR, <-, 4036-4966

centromere: CEN6/ARSH4, ->, 5070-5584

Notes: Restriction digests of the clone give the following sizes (kb): SacI/XbaI--5.1,0.5; EcoRI--5.6; KpnI--5.6.

- ATCC staff

ATCC 87333, p426 GAL1

Description: High copy number shuttle expression vector. Expression from the galactokinase (GAL1) promoter is tightly repressed by

glucose and is strongly induced by galactose.

- Nucleic Acids Res. 22: 5767-5768, 1994

Vector size (kb): 6.417

Markers: ampR, URA3

Cloning sites: SpeI BamHI SmaI EcoRI HindIII ClaI SalI XhoI

Construction: pRS426 (ATCC 77107), GAL1 promoter

Features (with orientation and position when available):

marker(s): URA3, ->, 200-1300

terminator: CYC1, ->, 2004-2264

MCS: XhoI...XpeI, ->, 2265-2322

promoter for expression: GAL1, <-, 2339-2799

marker(s): ampR, <-, 3917-4847

replicon: 2 micron, ->, 4947-6287

Notes: Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.2,2.8, 0.5; EcoRI--6.4; KpnI--6.4.

- ATCC staff

ATCC 87334, p413 GALL

Description: Low copy number shuttle expression vector. The galactokinase (GALL) promoter is a deletion variant of GAL1, which

lacks one of the 3 UAS elements required for full induction of the promoter by galactose. The promoter is tightly repressed by glucose

and is induced at a moderate level by galactose.

- Nucleic Acids Res. 22: 5767-5768, 1994

Vector size (kb): 5.626

Markers: ampR, HIS3

Cloning sites: XbaI SpeI BamHI SmaI EcoRI EcoRV ClaI SalI XhoI

Construction: pRS413, GALL promoter

Features (with orientation and position when available):

marker(s): HIS3, ->, 200-1395

terminator: CYC1, ->, 2077-2337

MCS: XhoI...XbaI, ->, 2337-2400

promoter for expression: GALL, <-, 2401-2831

marker(s): ampR, <-, 4078-5009

centromere: CEN6/ARSH4, ->, 5112-5626

Notes: Restriction digests of the clone give the following sizes (kb): SacI/XbaI--5.2,0.45; EcoRI--5.6; KpnI--4.5, 0.9.

- ATCC staff

ATCC 87335,p423 GALL

Description: High copy number shuttle expression vector. The galactokinase (GALL) promoter is a deletion variant of GAL1, which

lacks one of the 3 UAS elements required for full induction of the promoter by galactose. The promoter is tightly repressed by glucose

and is induced at a moderate level by galactose.

Product Information Sheet for ATCC®87670

American Type Culture Collection 800-638-6597 or 703-365-2700

P.O. Box 1549 Fax: 703-365-2750

Manassas, VA 20108 USA E-mail: tech@atcc.org

www.atcc.org Or contact your local distributor.

- 8 -

- Nucleic Acids Res. 22: 5767-5768, 1994

Vector size (kb): 6.459

Markers: ampR, HIS3

Cloning sites: SpeI BamHI SmaI EcoRI EcoRV ClaI SalI XhoI

Construction: pRS423, GALL promoter

Features (with orientation and position when available):

marker(s): HIS3, ->, 200-1395

terminator: CYC1, ->, 2077-2337

MCS: XhoI...SpeI, ->, 2337-2394

promoter for expression: GALL, <-, 2401-2831

marker(s): ampR, <-, 3982-4912

replicon: 2 micron, ->, 5012-6352

Notes: Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.2,2.8, 0.45; EcoRI--6.5; KpnI--5.5, 1.0.

- ATCC staff

ATCC 87336, p414 GALL

Description: Low copy number shuttle expression vector.The galactokinase (GALL) promoter is a deletion variant of GAL1, which

lacks one of the 3 UAS elements required for full induction of the promoter by galactose. The promoter is tightly repressed by glucose

and is induced at a moderate level by galactose.

- Nucleic Acids Res. 22: 5767-5768, 1994

Vector size (kb): 5.444

Markers: ampR, TRP1

Cloning sites: SpeI BamHI SmaI PstI EcoRI ClaI SalI XhoI

Construction: pRS414, GALL promoter

Features (with orientation and position when available):

marker(s): TRP1, ->, 200-1213

terminator: CYC1, ->, 1895-2155

MCS: XhoI...SpeI, ->, 2155-2212

promoter for expression: GALL, <-, 2219-2649

marker(s): ampR, <-, 3896-4826

centromere: CEN6/ARSH4, ->, 4930-5444

Notes: Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.2,1.9, 0.45; EcoRI--5.4; KpnI--5.4.

- ATCC staff

ATCC 87338, p415 GALL

Description: Low copy number shuttle expression vector. The galactokinase (GALL) promoter is a deletion variant of GAL1, which

lacks one of the 3 UAS elements required for full induction of the promoter by galactose.The promoter is tightly repressed by glucose

and is induced at a moderate level by galactose.

- Nucleic Acids Res. 22: 5767-5768, 1994

Vector size (kb): 6.677

Markers: ampR, LEU2

Cloning sites: XbaI SpeI BamHI SmaI PstI HindIII SalI XhoI

Construction: pRS415, GALL promoter

Features (with orientation and position when available):

marker(s): LEU2, ->, 200-2446

terminator: CYC1, ->, 3128-3388

MCS: XhoI...XbaI, ->, 3388-3451

promoter for expression: GALL, <-, 3452-3882

marker(s): ampR, <-, 5129-6059

centromere: CEN6/ARSH4, ->, 6163-6677

Notes: Restriction digests of the clone give the following sizes (kb): SacI/XbaI--6.3, 0.45; EcoRI--4.3, 2.3; KpnI--5.1, 1.6.

- ATCC staff

Product Information Sheet for ATCC®87670

American Type Culture Collection 800-638-6597 or 703-365-2700

P.O. Box 1549 Fax: 703-365-2750

Manassas, VA 20108 USA E-mail: tech@atcc.org

www.atcc.org Or contact your local distributor.

- 9 -

ATCC 87339, p425 GALL

Description: High copy number shuttle expression vector.The galactokinase (GALL) promoter is a deletion variant of GAL1, which

lacks one of the 3 UAS elements required for full induction of the promoter by galactose. The promoter is tightly repressed by glucose

and is induced at a moderate level by galactose.

- Nucleic Acids Res. 22: 5767-5768, 1994

Vector size (kb): 7.510

Markers: ampR, LEU2

Cloning sites: SpeI BamHI SmaI PstI HindIII SalI XhoI

Construction: pRS425, GALL promoter

Features (with orientation and position when available):

marker(s): LEU2, ->

terminator: CYC1, ->

MCS: SpeI...XhoI, <-

promoter for expression: GALL, <-

marker(s): ampR, <-

replicon: 2 micron, ->

Notes: Restriction digests of the clone give the following sizes (kb): BamHI--7.8;EcoRI--5.4, 2.3; SacI/XbaI--3.9, 3.2.

- ATCC staff

ATCC 87340, p416 GALL

Description: Low copy number shuttle expression vector. The galactokinase (GALL) promoter is a deletion variant of GAL1, which

lacks one of the 3 UAS elements required for full induction of the promoter by galactose.The promoter is tightly repressed by glucose

and is induced at a moderate levelby galactose.

- Nucleic Acids Res. 22: 5767-5768, 1994

Vector size (kb): 5.554

Markers: ampR, URA3

Cloning sites: XbaI SpeI BamHI SmaI EcoRI HindIII ClaI SalI XhoI

Construction: pRS416, GALL promoter

Features (with orientation and position when available):

marker(s): URA3, ->, 200-1323

terminator: CYC1, ->, 2005-2265

MCS: XhoI...XbaI, ->, 2265-2328

promoter for expression: GALL, <-, 2329-2759

marker(s): ampR, <-, 4006-4936

centromere: CEN6/ARSH4, ->, 5040-5554

Notes: Restriction digests of the clone give the following sizes (kb): BamHI--5.6;EcoRI--5.6; SacI/XbaI--5.1, 0.43.

- ATCC staff

ATCC 87341, p426 GALL

Description: High copy number shuttle expression vector.The galactokinase (GALL) promoter is a deletion variant of GAL1, which

lacks one of the 3 UAS elements required for full induction of the promoter by galactose. The promoter is tightly repressed by glucose

and is induced at a moderate level by galactose.

- Nucleic Acids Res. 22: 5767-5768, 1994

Vector size (kb): 6.387

Markers: ampR, URA3

Cloning sites: SpeI BamHI SmaI EcoRI HindIII ClaI SalI XhoI

Construction: pRS426 (ATCC 77107), GALL promoter

Features (with orientation and position when available):

marker(s): URA3, ->

terminator: CYC1, ->

MCS: SpeI...XhoI, <-

promoter for expression: GALL, <-

marker(s): ampR, <-

replicon: 2 micron, ->

Product Information Sheet for ATCC®87670

American Type Culture Collection 800-638-6597 or 703-365-2700

P.O. Box 1549 Fax: 703-365-2750

Manassas, VA 20108 USA E-mail: tech@atcc.org

www.atcc.org Or contact your local distributor.

- 10 -

Notes: Restriction digests of the clone give the following sizes (kb): BamHI--6.6; EcoRI--6.6; SacI/XbaI--3.5, 2.8, 0.45.

- ATCC staff

ATCC 87342, p413 GALS

Description: Low copy number shuttle expression vector.The galactokinase (GALS) promoter is a deletion variant of GAL1, which

lacks one and a half of the 3 UAS elements required for full induction of the promoter by galactose. The promoter is tightly repressed

by glucose and is induced at a moderate level by galactose.

- Nucleic Acids Res. 22: 5767-5768, 1994

Vector size (kb): 5.611

Markers: ampR, HIS3

Cloning sites: XbaI SpeI BamHI SmaI EcoRI EcoRV ClaI SalI XhoI

Construction: pRS413, GALS promoter

Features (with orientation and position when available):

marker(s): HIS3, ->, 200-1395

terminator: CYC1, ->, 2077-2337

MCS: XhoI...XbaI, ->, 2401-2816

promoter for expression: GALS, <-, 2401-2816

marker(s): ampR, <-, 4036-4993

centromere: CEN6/ARSH4, ->, 5097-5611

Notes: Restriction digests of the clone give the following sizes (kb): BamHI--5.6;EcoRI--5.6; SacI/XbaI--5.2, 0.4.

- ATCC staff

ATCC 87343, p423 GALS

Description: High copy number shuttle expression vector.The galactokinase (GALS) promoter is a deletion variant of GAL1, which

lacks one and a half of the 3 UAS elements required for full induction of the promoter by galactose. The promoter is tightly repressed

by glucose and is induced at a moderate level by galactose.

- Nucleic Acids Res. 22: 5767-5768, 1994

Vector size (kb): 6.444

Markers: ampR, HIS3

Cloning sites: SpeI BamHI SmaI EcoRI EcoRV ClaI SalI XhoI

Construction: pRS423, GALS promoter

Features (with orientation and position when available):

marker(s): HIS3, ->, 200-1395

terminator: CYC1, ->, 2077-2337

MCS: XhoI...SpeI, ->, 2337-2394

promoter for expression: GALS, <-, 2401-2816

marker(s): ampR, <-, 3967-4897

replicon: 2 micron, ->, 4997-6337

NOTES:Restriction digests of the clone give the following sizes (kb): BamHI--6.2; EcoRI--6.2; PstI--5.0, 1.2; SacI/XbaI--3.2, 2.8.

- ATCC staff

ATCC 87344, p414 GALS

Description: Low copy number shuttle expression vector.The galactokinase (GALS) promoter is a deletion variant of GAL1, which

lacks one and a half of the 3 UAS elements required for full induction of the promoter by galactose. The promoter is tightly repressed

by glucose and is induced at a moderate level by galactose.

- Nucleic Acids Res. 22: 5767-5768, 1994

Vector size (kb): 5.429

Markers: ampR, TRP1

Cloning sites: SpeI BamHI SmaI PstI EcoRI ClaI SalI XhoI

Construction: pRS414, GALS promoter

Features (with orientation and position when available):

marker(s): TRP1, ->, 200-1213

terminator: CYC1, ->, 1895-2155

MCS: XhoI...SpeI, ->, 2155-2212

Product Information Sheet for ATCC®87670

American Type Culture Collection 800-638-6597 or 703-365-2700

P.O. Box 1549 Fax: 703-365-2750

Manassas, VA 20108 USA E-mail: tech@atcc.org

www.atcc.org Or contact your local distributor.

- 11 -

promoter for expression: GALS, <-, 2219-2634

marker(s): ampR, <-, 3881-4811

centromere: CEN6/ARSH4, ->, 4915-5429

Notes:Restriction digests of the clone give the following sizes (kb): BamHI--5.4;EcoRI--5.4; SacI/XbaI--3.2, 1.8, 0.41.

- ATCC staff

ATCC 87345, p424 GALS

Description: High copy number shuttle expression vector. The galactokinase (GALS) promoter is a deletion variant of GAL1, which

lacks one and a half of the 3 UAS elements required for full induction of the promoter by galactose. The promoter is tightly repressed

by glucose and is induced at a moderate level by galactose.

- Nucleic Acids Res. 22: 5767-5768, 1994

Vector size (kb): 6.262

Markers: ampR, TRP1

Cloning sites: SpeI BamHI SmaI PstI EcoRI ClaI SalI XhoI

Construction: pRS424, GALS promoter

Features (with orientation and position when available):

marker(s): TRP1, ->, 200-1213

terminator: CYC1, ->, 1895-2155

MCS: XhoI...SpeI, ->, 2155-2212

promoter for expression: GALS, <-, 2219-2634

marker(s): ampR, <-, 3785-4715

replicon: 2 micron, ->, 4815-6155

Notes: Restriction digests of the clone give the following sizes (kb): BamHI--6.2; XhoI--6.2; SacI/XbaI--3.2, 1.7, 1.0, 0.42.

- ATCC staff

ATCC 87346, p415 GALS

Description: Low copy number shuttle expression vector. The galactokinase (GALS) promoter is a deletion variant of GAL1, which

lacks one and a half of the 3 UAS elements required for full induction of the promoter by galactose. The promoter is tightly repressed

by glucose and is induced at a moderate level by galactose.

- Nucleic Acids Res. 22: 5767-5768, 1994

Vector size (kb): 6.662

Markers: ampR, LEU2

Cloning sites: XbaI SpeI BamHI SmaI PstI HindIII SalI XhoI

Construction: pRS415, GALS promoter

Features (with orientation and position when available):

marker(s): LEU2, ->, 200-2446

terminator: CYC1, ->, 3128-3388

MCS: XhoI...XbaI, ->, 3388-3451

promoter for expression: GALS, <-, 3452-3867

marker(s): ampR, <-, 5114-6044

centromere: CEN6/ARSH4, ->, 6148-6662

Notes: Restriction digests of the clone give the following sizes (kb): BamHI--6.6; EcoRI--4.2, 2.4; SacI/XbaI--6.2, 0.42.

- ATCC staff

ATCC 87347, p425 GALS

Description: High copy number shuttle expression vector.The galactokinase (GALS) promoter is a deletion variant of GAL1, which

lacks one and a half of the 3 UAS elements required for full induction of the promoter by galactose. The promoter is tightly repressed

by glucose and is induced at a moderate level by galactose.

- Nucleic Acids Res. 22: 5767-5768, 1994

Vector size (kb): 7.495

Markers: ampR, LEU2

Cloning sites: SpeI BamHI SmaI PstI HindIII SalI XhoI

Construction: pRS425, GALS promoter

Features (with orientation and position when available):

Product Information Sheet for ATCC®87670

American Type Culture Collection 800-638-6597 or 703-365-2700

P.O. Box 1549 Fax: 703-365-2750

Manassas, VA 20108 USA E-mail: tech@atcc.org

www.atcc.org Or contact your local distributor.

- 12 -

marker(s): LEU2, ->, 200-2446

terminator: CYC1, ->, 3128-3388

MCS: XhoI...SpeI, ->, 3388-3445

promoter for expression: GALS, <-, 3452-3867

marker(s): ampR, <-, 5018-5948

replicon: 2 micron, ->, 6048-7388

Notes: Restriction digests of the clone give the following sizes (kb): BamHI--8.0; EcoRI--5.2, 2.3; SacI/XbaI--3.9, 3.3, 0.44.

- ATCC staff

ATCC 87348, p416 GALS

Description: Low copy number shuttle expression vector.The galactokinase (GALS) promoter is a deletion variant of GAL1, which

lacks one and a half of the 3 UAS elements required for full induction of the promoter by galactose. The promoter is tightly repressed

by glucose and is induced at a moderate level by galactose.

- Nucleic Acids Res. 22: 5767-5768, 1994

Vector size (kb): 5.539

Markers: ampR, URA3

Cloning sites: XbaI SpeI BamHI SmaI EcoRI HindIII ClaI SalI XhoI

Construction: pRS416, GALS promoter

Features (with orientation and position when available):

marker(s): URA3, ->, 200-1323

terminator: CYC1, ->, 2005-2265

MCS: XhoI...XbaI, ->, 2265-2323

promoter for expression: GALS, <-, 2329-2744

marker(s): ampR, <-, 3991-4921

centromere: CEN6/ARSH4, ->, 5025-5539

Notes: Restriction digests of the clone give the following sizes (kb): BamHI--5.6; HindIII--5.6; EcoRI--5.6; SacI/XbaI--5.2, 0.4.

- ATCC staff

ATCC 87349, p426 GALS

Description: High copy number shuttle expression vector. The galactokinase (GALS) promoter is a deletion variant of GAL1, which

lacks one and a half of the 3 UAS elements required for full induction of the promoter by galactose. The promoter is tightly repressed

by glucose and is induced at a moderate level by galactose.

- Nucleic Acids Res. 22: 5767-5768, 1994

Vector size (kb): 6.372

Markers: ampR, URA3

Cloning sites: SpeI BamHI SmaI EcoRI HindIII ClaI SalI XhoI

Construction: pRS426 (ATCC 77107), GALS promoter

Features (with orientation and position when available):

marker(s): URA3, ->, 200-1300

terminator: CYC1, ->, 2004-2264

MCS: XhoI...SpeI, ->, 2265-2322

promoter for expression: GALS, <-, 2339-2754

marker(s): ampR, <-, 3872-4802

replicon: 2 micron, ->, 4902-6242

Notes: Restriction digests of the clone give the following sizes (kb): BamHI--6.6;EcoRI--6.6; SacI/XbaI--3.4, 2.9, 0.48.

- ATCC staff

Product Information Sheet for ATCC®87670

American Type Culture Collection 800-638-6597 or 703-365-2700

P.O. Box 1549 Fax: 703-365-2750

Manassas, VA 20108 USA E-mail: tech@atcc.org

www.atcc.org Or contact your local distributor.

- 13 -

MAPS:

Product Information Sheet for ATCC®87670

American Type Culture Collection 800-638-6597 or 703-365-2700

P.O. Box 1549 Fax: 703-365-2750

Manassas, VA 20108 USA E-mail: tech@atcc.org

www.atcc.org Or contact your local distributor.

- 14 -

Product Information Sheet for ATCC®87670

American Type Culture Collection 800-638-6597 or 703-365-2700

P.O. Box 1549 Fax: 703-365-2750

Manassas, VA 20108 USA E-mail: tech@atcc.org

www.atcc.org Or contact your local distributor.

- 15 -

Product Information Sheet for ATCC®87670

American Type Culture Collection 800-638-6597 or 703-365-2700

P.O. Box 1549 Fax: 703-365-2750

Manassas, VA 20108 USA E-mail: tech@atcc.org

www.atcc.org Or contact your local distributor.

- 16 -

Product Information Sheet for ATCC®87670

American Type Culture Collection 800-638-6597 or 703-365-2700

P.O. Box 1549 Fax: 703-365-2750

Manassas, VA 20108 USA E-mail: tech@atcc.org

www.atcc.org Or contact your local distributor.

- 17 -

Product Information Sheet for ATCC®87670

American Type Culture Collection 800-638-6597 or 703-365-2700

P.O. Box 1549 Fax: 703-365-2750

Manassas, VA 20108 USA E-mail: tech@atcc.org

www.atcc.org Or contact your local distributor.

- 18 -

BIOSAFETY LEVEL: 1

Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: Biosafety in

Microbiological and Biomedical Laboratories, 4th ed. HHS Publication No. (CDC) 93-8395. U.S. Department of Health and Human Services, Centers for

Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 1999. The entire text is available online at

www.cdc.gov/od/ohs/biosfty/bmbl4/bmbl4toc.htm.

ATCC® WARRANTY:

The viability of ATCC® products is warranted for 30 days from the date of shipment. If you feel there is a problem with this product, contact

Technical Services by phone at 800-638-6597 or 703-365-2700 or by e-mail at tech@atcc.org. Or you may contact your local distributor.

DISCLAIMERS:

This product is intended for laboratory research purposes only. It is not intended for use in humans.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or

representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant

that such information has been confirmed to be accurate.

This product is sent with the condition that you are responsible for its safe storage, handling, and use. ATCC is not liable for any damages or

injuries arising from receipt and/or use of this product. While reasonable effort is made to insure authenticity and reliability of strains on deposit, ATCC is

not liable for damages arising from the misidentification or misrepresentation of cultures.

Please see the enclosed Material Transfer Agreement (MTA) for further details regarding the use of this product. The MTA is also available on our

Web site at www.atcc.org.

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ATCC is a registered trademark of the American Type Culture Collection.

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