广宿主质粒载体IncQ型pDSK519,pGSS33图谱序列抗性

Product Information

Recovery

1. Obtain an LB agar plate with the appropriate antibiotic.

2. Using a sterile pipette tip, touch the bacteria growing within the punctured area of the stab culture. (A sterilized wire loop or sterile toothpick can be used in place of a sterile pipette tip.)

3. Run this tip lightly over a section of the plate, as shown in the figure, to create streak #1.

4. Using another sterile pipette tip, pass through streak #1 and spread the bacteria over a second section of the plate, to create streak #2.

5. Using a third sterile pipette tip, pass through streak #2 and spread the bacteria over the last section of the plate, to create streak #3.

6. Grow overnight in a 37 ^o C incubator (unless a different growth temperature is indicated on the plasmid datasheet).

7. In the morning, single colonies should be visible. If the bacterial growth is too dense, re-streak onto a new agar plate to obtain single colonies.

Sequence:

LOCUS       JQ173098                8699 bp    DNA     circular SYN 04-APR-2012
DEFINITION  Cloning vector pDSK519, complete sequence.
ACCESSION   JQ173098
VERSION     JQ173098.1  GI:382976187
KEYWORDS    .
SOURCE      Cloning vector pDSK519
  ORGANISM  Cloning vector pDSK519
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 8699)
  AUTHORS   Keen,N.T., Tamaki,S., Kobayashi,D. and Trollinger,D.
  TITLE     Improved broad-host-range plasmids for DNA cloning in gram-negative
            bacteria
  JOURNAL   Gene 70 (1), 191-197 (1988)
   PUBMED   2853689

The broad-host-range IncQ group plasmids pDSK519 and pGSS33