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pGL4.29[luc2P/CRE/Hygro]质粒载体图谱序列抗性

  • 价  格:¥1920
  • 货  号:3497453
  • 产  地:北京
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出品公司: Promega
质粒类型: 启动子报告载体
克隆方法: 多克隆位点,限制性内切酶
载体大小: 6084 bp
载体抗性: Ampicillin (氨苄青霉素)
筛选标记: Hygromycin (潮霉素)
备注: Vector with a minimal promoter and a cAMP response element for studying cell signaling using destabilized luciferase.
产品编号 产品名称 规格 价格
Biovector102047 pGL4.29[luc2P/CRE/Hygro]

10ug质粒

¥1920.00
 
 

The pGL4.29[luc2P/CRE/Hygro] Vector(a–f) contains a cAMP response element (CRE) that drives the transcription of the luciferase reporter gene luc2P (Photinus pyralis). luc2P is a synthetically-derived luciferase sequence with humanized codon optimization that is designed for high expression and reduced anomalous transcription. The luc2P gene contains hPEST, a protein destabilization sequence. The protein encoded by luc2P responds more quickly than the protein encoded by the luc2 gene upon induction. The vector backbone contains an ampicillin resistance gene to allow selection in E. coli and a mammalian selectable marker for hygromycin resistance.

提供荧光定位载体pEGFP-actin,pECFP-ER,pEGFP-N1,pIRES2-DsRed.

Taq,Pfu高表达载体菌株KOD, T4连接酶高表达质粒载体,高产基因工程菌株、酶基因KOD,T4 kinase激酶,Tth反转录酶MMLV,Klentaq PCR酶,Cre酶,RNA inhibitor抑制酶,DL2000质粒 荧光蛋白表达载体荧光定位质粒报告基因载体pEGFP,pEYFP,pECFP,pDsRED, pmCherry,pAcGFP,hrGFP,ZsGreen,pDsRed1-C1, pDsRed1-N1, pDsRED-Monomer-N1, pEYFP-C1, pEYFP-N1, pEGFP-1,pEGFP-C1, pEGFP-N1, pEGFP-N3, pcDNA-EGFP1,pECFP-N1,pECFP-C1, pAcGFP1-1 pDsRED2-ER, pDsRED2-Mito, pDsRED2-Nuc,pDsRED2-Peroxi , pEYFP-Golgi, pEYFP-Mem, pEYFP-ER , pEGFP-Actin, pECFP-ER, pIRES2-EGFP, pLEGFP-C1,pLEGFP-N1,pDsRED-Express-N1,pIRES2-DsRed,pIRES-AcGFP1,pmCherry-C1,N1 启动子增强子报告载体(Promega,带有Luc荧光报告基因): pGL3-basic,pGL3-control,pGL3-enhancer,pGL3-promoter,pRL-TK,pRL-CMV,pRL-SV40, 信号通路报告载体(Clontech): pGAS-TA-Luc,pSTAT3-TA-Luc, pISRE-TA-Luc, pTA-Luc,pIκB-EGFP, pNFAT-TA-Luc,pCaspase3-sensor,pAP1(PMA)-Luc 信号通路报告载体(Promega): pGL4.26[luc2P/minP/Hygro],pGL4.29[luc2P/CRE/Hygro],pGL4.30[luc2P/NFAT-RE/Hygro],pGL4.75 信号通路报告载体(Stratagene): p53-Luc,pAP-1-Luc, pNF-κB-Luc,pSRE-Luc, pFA2-Elk1,pFC-MEKK,pFR-luc pJRD215,pJN105,pME6032,Cos载体pLAFR3,pMP2444(GFP), pHY300PLK,pRT102,pRL1063a, 转座 子载体pUT-miniTn5,pMGS100, pWHM10,pKC1139,pSET152, pOJ260,pPG611.1,pPG612.1, 杂交系统: 哺乳动物双杂交系统 Promega Vectors:pACT,pBIND Contorl plasmid:pACT-MyoD,pBIND-Id Reporter plasmid:pG5 luc Stratagene Vectors:pCMV-BD,pCMV-AD Contorl plasmid:pBD-p53,pBD-NF−κB,pAD-SV40T,pAD-TRAF Reporter plasmid:pFR-luc 酵母双杂交系统 Clontech Vectors:pGBKT7,pGADT7, Positive contorl:pCL1,pGBKT7-53,pGADT7-T Negative control:pGBKT7-Lam Strain:AH109 Vector:pACT2 AD Stratagene Vectors:pSos,pMyr Control plasmid:pSos MAFB, 酵母单杂交系统 Clontech Vector:pHis2,pHISi,pHISi-1,pLacZi,pGAD424 ,pGAD53m,p53BLUE,p53HIS 酵母三杂交系统 Clontech Vector:pBridge Strains:Y2H Gold Yeast strain,Y187 诱导系统: 蜕皮激素诱导表达系统 Invitrogen Vectors:pIND,pVgRxR LacSwith II哺乳动物诱导表达系统 Stratagene Vectors:pOPRSVI,pOPI3CAT,pCMVLacI GeneSwith 哺乳动物诱导系统 Invitrogen Vectors:pSwitch,pGene v5-His B (BamHI-EcoRI) 四环素诱导系统 Invitrogen Vectors:pcDNA4/TO/Myc-His A,pcDNA4/TO/Myc-His B, pcDNA4/TO/Myc-His C, Contorl plasmid:pcDNA4/TO/Myc-His/LacZ Regulatory plasmid:pcDNA6/TR Clontech Vectors:pTet-On,pTet-Off,pTRE2,pTRE2 hyg(mcs区含有插入片段),pTK-hyg pRevTet-On,pRevTet-Off,pRevTRE pTet on advanced,pTRE Tight,pTRE Tight Luciferase pCMV-Tet3G,pTRE3G,pTRE3G-Luc, Takara分子伴侣系统: Vectors:pTf16,pG-Tf2,pG-KJE8,pKJE7 腺病毒系统: Stratagene Vectors:pShuttle-CMV,pShuttle,pAdTrack-CMV,pAdTrack,pAdEasy-1,pShuttle-IRES-hrGFP-2,pAdTrack-CMV/beta-gal Strains:BJ5183,AdEasy-1 Invitrogen Vector:pAd/BLOCK-iT-DEST RNAi Gateway Vector, microbix公司AdMax腺病毒: pEarleygate103,pDC315 腺相关病毒系统: Stratagene Vector:pAAV-MCS,pAAV-RC,pHelper,pAAV-LacZ,pAAV-IRES-hrGFP,pCMV-MCS, pAAV-EGFP,pAAV-U6-ires-hrGFP,pAAV-Rc1型,pAAV-Rc8型,pAAV-Rc9型, 逆病毒系统: RNAi Clontech Vectors:RNAi-Ready pSIREN-RetroQ, RNAi-Ready pSIREN-RetroQ-DsRed-Express RNAi-Ready pSIREN-RetroQ-ZsGreen(Luciferase shRNA Annealed Oligonucleotide), Ambion Vectors:pSilencer5.1-U6-Retro,pSilencer5.1-H1-Retro 基因表达载体 Clontech Vectors:pRetroX-IRES-ZsGreen1,pLNCX2,pQCXIH,pMSCVpuro,pMSCVneo,pMSCV-EGFP,pLXSN pRetroX-IRES-DsRed Express, Addgene Vectors:,pSuper retro puro,pBabe puro 逆病毒包装载体 Stratagene Vectors:pVPack-GP,pCMV VSV G Addgene Vectors:pCMV-Gag-pol,pCMV-VSV-G,pCL Ampho 慢病毒系统: RNAi载体 Addgene Vectors:pSicoR,pSicoR pGK puro,pLL 3.7,pLKO.1 - TRC Cloning,pLKO.3G, pPRIME-TET-GFP-FF3,pPRIME-TREX-GFP-FF3,pLVTH,pLVTHM Clontech Vectors:pLVX-shRNA1,pLVX-shRNA2 SBI Vectors:pSIH1-H1-CoGFP,pPS-rtTA3R-Puro,pPS-rtTA3R-RFP,pPS-TRE-GFP-miR,pPS-TRE-RFP-miR Open Biosystems Vectors:pGIPZ empty,pGIPZ control,pTRIPZ empty,pTRIPZ control 基因表达载体 Clontech Vectors:pLVX-DsRed-Monomer-N1,pLVX-AcGFP1-N1,pLVX-IRES-ZsGreen1 pLVX-IRES-neo,pLVX-IRES-td Tomato,pLVX-IRES-puro,pLVX-puro, pLVX-Tight-Puro,pLVX-Tet-On-Advanced Invitrogen Vector: pLenti4 TO/V5 DEST,pLenti6 V5 DEST,pLenti6-UbC-V5-DEST Addgene Vector:pWPXL,FUGW,pLVTH,pLVTHM 慢病毒包装载体 Invitrogen Vectors:pLp1,pLp2,pLp /VSVG Strain:Stbl3,Stbl4 Cell biolabs Vectors:pCgpV Clontech Vector:pVSV G Addgene Second generation: Vectors:pCMV-dR8.91,pCMV-VSV-G Second generation: Vectors:pMD2.G,pSPAX2 Third generation: Vectors:pMDLg pRRE,pRSV-rev,pCMV-VSV-G(pMDLg/pRRE + pRSV-Rev + pMD2.G) iPS慢病毒载体 Cell biolabs Vectors:pLentG-KOSM Addgene Vectors:FUW-M2rtTA,TetO-FUW-OSKM PiggyBac转座子系统 Vector:HA-mPB,PiggyBac 5’LTR (pMD18T vector), PiggyBac 3’LTR (pMD18T vector) 信号通路报告系统: Clontech Vectors:pGAS-TA-Luc,pSTAT3-TA-Luc,pISRE-TA-Luc,pTA-Luc, pIκB-EGFP,pNFAT-TA-Luc,pCaspase3-sensor,pAP1(PMA)-TA-Luc pCRE-Luc,pGRE-Luc,pHSE-Luc Stratagene Vectors:p53-Luc,pAP-1-Luc,pNF-κB-Luc,pSRE-Luc,pFA2-Elk1,pFC-MEKK,pFB-luc Promega Vectors:pGL4.10,pGL4.26,pGL4.29,pGL4.30,pGL4.75,pGL4.19 Millipore Vectors:TOPFlash,FOPFlash,SuperTopFlash 国产: pZRT1-Luc RNAi系统: Ambion Vectors: pSilencer 1.0-U6(pBS/U6),pSilencer 2.1-U6 hygro,pSilencer 3.1-H1 hygro,pSilencer 3.1-H1 neo,pSilencer 4.1-CMV neo,pSilencer 4.1-CMV puro, Reporter plasmid:pMIR-REPORT Luciferase Promega Reproter plasmid:psiCHECK-2,pmir GLO Oligoengine Vector:pSuper-puro,pSUPER retro Puro Other Vector:pGenesil-1, pucrnai, Clontech Vector:pmR mCherry SBI Vector:pmiRZip anti-microRNA 酵母表达系统: 酿酒酵母 Invitrogen Vectors:pYES2,pYES6/CT,pYES2/CT/α-factor,pYES3/CT Strain:INVSc1 毕赤酵母 Invitrogen Vectors:pGAPZα A,pPICZα A,pPICZα B,pPICZα C pPIC9K,pPIC3.5K pPICZ A,pPICZ B,pPICZ C, Strains:X33,KM71,KM71H,GS115,SMD1163,SMD1168 Invitrogen Vector:pPink HC,pPink LC Strain:Pichiapink-1,Pichiapink-4 Gateway系统: Invitrogen Vectors:pENTR 1A,pENTR3C,pDONR221, pcDNA6.2-GWEmGFP-miR negative, Regulatory plasmid:pLenti6/TR Strain:DB3.1 表面展示系统: 酵母表面展示 Invitrogen Vector:pYD1 Strain:EBY100 哺乳动物表面展示 Invitrogen Vector:pDisplay 噬菌体表面展示 Vector:pCantab 5e Strain:HB2151,TG1,M13KO7(in TG1) 重组蛋白乳汁表达系统: Invitrogen Vector:pBC1 分泌型真核表达载体: Invitrogen Vector:PsecTag2A,PsecTag2C 绝缘子检测系统: Invivogen Vector:pWHERE 果蝇细胞表达系统: Invitrogen Vector:pAc5.1 V5-His B,pCo hygro 哺乳动物表达系统: Invitrogen Vectors:pcDNA3.1(+)neo,pcDNA3.1(-)neo ,pcDNA3.1(+)hyg,pcDNA3.1(+)zeo, pcDNA3.1/GS,pcDNA3.1(-)/myc-His A,pcDNA4/HisMax A,pcDNA4/HisMax B,pcDNA4/HisMax C,pcDNA4/HisMax lacZ,pcDNA6-Myc/His B,pSecTag2 A,pVAX1,PVAX1-lacZ ,pBudCE4.1,pTracer CMV2, pCEP4,pUB6/V5 His A,pUB6/V5 His B,pUB6/V5 His C,pUB6/V5 His lacZ,pSFV1,pEF1/myc-His B,pCDNA3.0,pCDNA3.0/3C-myc,pCDNA3.0/3flag,pCDNA3.0/3HA,pCDNA3.0/5flag,pCDNA3.0/5HA,pCDNA3.0/beta-gal,pCDNA3.0/EGFP/C,pCDNA3.0/EGFP/N,pcDNA3.1D/v5-His,pBAD/His A,pEF6/Myc-HisB, pEF6/Myc-HisC Stratagen Vector:pCMV-Tag 2 B,pCMV-Tag 5B,pNTAP-B, pBK-CMV,pIRES-hrGFP-1a,pIRES-hrGFP-2a, pSG5 Promega Vectors:pCI-neo,pCI,pSI Clontech Vectors: pCMV-myc,pCMV-HA,pIRES,pIRES neo,pIRES neo2,pIRES hyg3, pIRES neo3,pIRES puro3(EcoRI-NotI之间连入Mus musculus CD1d1 antigen), pIRES2-EGFP,pIRES2-DsRed-Express ,pIRES puro2, Sigma Vectors:pFLAG CMV2,pFlag-CMV1 Invivogen Vectors:pVitro2-neo-mcs Addgene Vectors:pCAGGS Invivogen Vector:pFUSE-CHIg-hG1(modified) 昆虫细胞表达系统: Invitrogen Vectors:pFastBac1,pFastBac1-Gus,pFastBacHT A,pFastBacHT B,pFastBacHT C,pFastBacHT-CAT,pFastBacDual,pBlueBacHis2 A,pBlueBacHis2 B, pBlueBacHis2 C,pVL1392,pVL1393, Strain:DH10Bac Vectors:pIZT/V5-His,pMIB v5-His B Cell:sf9 , High Five MultiBac Expression System Vectors:pFBDM,pUCDM,pBADZ-His6Cre, Strains:DH10MultiBac,BW23473 Cell:sf9 植物表达载体: pCAMBIA 1300,pCAMBIA 1301,pCAMBIA 1305,pcambia 1302, pBI 121 荧光蛋白报告系统: Clontech Vectors:pDsRed1-N1,pDsRed1-C1,pDsRed2-N1,pDsRed2-C1pDsRED-Monomer-N1,pmCherry-N1,pmCherry-C1, pEYFP-N1,pEYFP-C1,pEGFP-N1,pEGFP-C1,pEGFP-N2,pEGFP-C2,pEGFP-N3,pEGFP-C3,pEGFP-1,pAcGFP1-1,pECFP-N1,pECFP-C1,pDsRED2-ER,pDsRED2-Mito,pDsRED2-Nuc,pDsRED2-Peroxi,pEYFP-Golgi,pEYFP-Mem,pEYFP-ER,pEYFP actin,pEYFP tub,pEGFP-Actin,pECFP-ER,pLEGFP-N1,pLEGFP-C1, pGFPuv, pECFP-Mito,pDsRed2-1,pPAmCherry-Tubulin FRET assay Vectors:Dkeima fluorescent protein,mAmetrine, 启动子增强子报告系统: Promega Vectors:pGL3-basic,pGL3-control,pGL3-enhancer,pGL3-promoter, pRL-TK,pRL-CMV,pRL-SV40 NEB Vectors:pGluc-basic 原核表达系统: Novagen Vectors:pET-11a(+),pET-17b(+),pET-19b(+),pET-20b(+),pET-21a(+),pET-22b(+), pET-26b(+),pET-27b(+),pET-28a(+),pET-28b(+),pET-29a(+),pET-30a(+), pET-32a(+),pET-39b(+),pET-40b(+),pET-42a(+), pET49b(+),pET50b(+), pETduet-1,pACYCDuet-1,pRSFDuet-1,pCDFDuet-1, pET43.1a+ Strains:BL21,BL21(DE3),BL21(DE3)plysS,Rosetta(DE3),Rosetta-gami 2(DE3)pLysS, Rosetta-gami B(DE3)pLysS,BLR(DE3), BL21(DE3)gold,Rosetta Blue(DE3) Takara Vectors:pCold I,pCold TF NEB Vectors:pMAL-c2x,pMAL-c4x,pMAL-p4x Biosee Vector:pLLP-OmpA,pLLP-STII,pMBP-P,pMBP-C,pET-GST,pET-Trx, pET-His,pTrc-CKS,pET-DsbA Stratagene Vector:pBluescript II KS(+),pBluescript II KS(+)-EGFP,pBluescript II sk(+), Strain:BL21 codonplus(DE3)RIPL Invitrogen Strain:BL21(AI) Qiagen Vector:pQE 30,PQE 31,PQE 60,PQE 70,PQE 9, Strain:M15 NEB Vectors:pTWIN1,pTYB11,pMXB10,pTXB1 Strain:ER2566 Amersham Vectors:pGEX-4T-1,PGEX-5X-1,pGEX-6P-1,pGEX-2TK,pGEX-5X-2 Invitrogen Vectors:pBADHis A,pBADHis B,pBADHis C, Lucigen Strain:C41(DE3),C43(DE3) 其他: Vectors:PHB201,pALTER-1 温控表达系统: PBV221 基因载体: Invitrogen Vector:pOG44 Invivogen Vectors:pORF-lacZ,pORF-HSV1tk 克隆载体: Promega Vectors:pUC18,pUC19,pBR322,pSP64,pSP73,pCR-Blunt(环状),pUCm-T(环状),pBS-T(环状),PBS-T S (环状),pSV-beta-gal,pRS315-Flag,pRS315-myc, 柯斯载体: Stratagene Vector:SuperCos1 同源重组载体: Vector:pSIM5,pSIM6 其他: pSAL1,pspT18,pspT18-Neo,pspT19,pspT19-Neo, 克隆菌株: Stratagene Strains:DH5α,JM109,JM110,TOP10,BL21,GM2163,BLT5403,BLT5615,XL1-Blue,XL1-Blue MRF’,XLOLR,XL2-Blue MRF’,Mach1 T1,OmniMAX2 T1 Phage-Resistant Cells,ExAssist interference-resistant helper phage, R408 Interference Resistant Helper Phage, XL10 Gold,EZ10 基因: Genes: human TERT,Tag,Pfu (pET16b(+): NdeI-BamHI 2325bp),KOD (pET21b+),T4 DNA ligase (pET15b+,优化的T4 DNA连接酶),hOct4,hSox2,hKlf4,hC-Myc,mOct4,mSox2,mKlf4,mC-Myc,human CD40L,human DHFR,human Hspa1a,human IgG1 Fc,mouse GM-CSF,mouse IFN-gamma,mouse IL4,mouse IL18,Cre酶,rat 91ES8R(X80155),human HIF1A(NM_001530),human GADD45A,human GADD45B,human GADD45G,human Hegf(AAA60744),human Vegf165(AF486837),human FGF5(NM_033143),human P53(NM_000546),human VEGF121(AF214570),human CXCR4,human HSP40,human UBE2I,human FKBP38,human FKBP12,human IP-10,human arr2,human arr1,human HVEM(NM_003820.2),human PPP1CC(NM_002710),human RICTOR(NM_152756),human SOCS2(NM_003877),human FADD(NM_003824.3),human ID1(NM_002165.2),human STAT3(NM_139276),human NOTCH2(NM_024408.2),human KRT19(NM_002276),human KLK3(NM_001648),human CEACAM5(NM_004363),human CGB(NM_000737),human GRWD1(NM_031485),human RELT(NM_032871),human RELT(NM_152222),human AFP(NM_001134),human ASB9(NM_001031739),human ASB9(NM_024087),human ASB9(NM_001168530),human ASB9(NM_001168531),human KLF17(NM_173484),human RELT(NM_032871),human RELT(NM_152222),human KLK3(NM_001648),human EMA6C (NM_030913.3),human DUSP8 (NM_004420.2),human SAPS2 (NM_014678.3),human RPS6,human PPP6R2(NM_014678),human beclin1,human ATG5,human ATG12,human SLC10A1,human SLC10A2 ,human RIPK3(NM_006871.3),human RIPK1,pET-24a-SacB,human IER5(NM_016545),

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