pSQT1601质粒载体CRISPR/Cas9系统Csy4，FokI-dCas9表达载体图谱序列抗性说明书

价　　格：¥1920
货　　号：pSQT1601
产　　地：北京

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pSQT1601质粒载体CRISPR/Cas9系统Csy4，FokI-dCas9表达载体

pSQT1601
(BioVector-053369)

PURPOSE
human codon optimized Csy4 and FokI-dCas9 expression plasmid
DEPOSITING LAB
Keith Joung
PUBLICATION
Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2908.

BACKBONE

Vector backbone
pCAG-CFP
(Search Vector Database)
Vector type
Mammalian Expression, CRISPR

GROWTH IN BACTERIA

Bacterial Resistance(s)
Ampicillin
Growth Temperature
37°C
Growth Strain(s)
XL1 Blue
Copy number
High Copy

SEQUENCE INFORMATION

Depositor Sequences
Full (1)
Addgene Sequences
Partial (3)

GENE/INSERT

Gene/Insert name
hCsy4-T2A-NLS-hFokI-dCas9-NLS
Alt name
Csy4
Alt name
FokI-dCas9
PromoterCAG

CLONING INFORMATION

Cloning methodUnknown
5′ sequencing primerpCAG-F
3′ sequencing primerBglob-pA-R

(Common Sequencing Primers)

RESOURCE INFORMATION

Terms and Licenses
- UBMTA

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

For your Materials & Methods section:
pSQT1601 was a gift from Keith Joung (Addgene plasmid # 53369)
For your References section:
Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing. Tsai SQ, Wyvekens N, Khayter C, Foden JA, Thapar V, Reyon D, Goodwin MJ, Aryee MJ, Joung JK. Nat Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2908.10.1038/nbt.2908 PubMed 24770325