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pSQT1601质粒载体CRISPR/Cas9系统Csy4,FokI-dCas9表达载体
pSQT1601
(BioVector-053369)
- PURPOSEhuman codon optimized Csy4 and FokI-dCas9 expression plasmid
- DEPOSITING LAB
BACKBONE
- Vector backbonepCAG-CFP
- Vector typeMammalian Expression, CRISPR
GROWTH IN BACTERIA
- Bacterial Resistance(s)Ampicillin
- Growth Temperature37°C
- Growth Strain(s)XL1 Blue
- Copy numberHigh Copy
SEQUENCE INFORMATION
- Depositor Sequences
- Addgene Sequences
GENE/INSERT
- Gene/Insert namehCsy4-T2A-NLS-hFokI-dCas9-NLS
- Alt nameCsy4
- Alt nameFokI-dCas9
PromoterCAG
CLONING INFORMATION
Cloning methodUnknown
5′ sequencing primerpCAG-F
3′ sequencing primerBglob-pA-R
(Common Sequencing Primers)
RESOURCE INFORMATION
- Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:
pSQT1601 was a gift from Keith Joung (Addgene plasmid # 53369)For your References section:
Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing. Tsai SQ, Wyvekens N, Khayter C, Foden JA, Thapar V, Reyon D, Goodwin MJ, Aryee MJ, Joung JK. Nat Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2908.10.1038/nbt.2908 PubMed 24770325
- 公告/新闻